黄枝油杉遗传多样性的ISSR分析

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引用本文:	谢伟玲，柴胜丰*，蒋运生，唐健民，陈宗游，邹蓉，韦霄.黄枝油杉遗传多样性的ISSR分析[J].广西植物,2017,37(1):36-41.[点击复制]
	XIE Wei Ling, CHAI Sheng Feng*, JIANG Yun Sheng, TANG Jian Min, CHEN Zong You, ZOU Rong, WEI Xiao.ISSR analysis on genetic diversity of Keteleeria calcarea[J].Guihaia,2017,37(1):36-41.[点击复制]

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黄枝油杉遗传多样性的ISSR分析
谢伟玲，柴胜丰*，蒋运生，唐健民，陈宗游，邹蓉，韦霄
广西喀斯特植物保育与恢复生态学重点实验室，广西壮族自治区中国科学院　广西植物研究所，广西桂林 541006

摘要:

该研究采用ISSR分子标记对黄枝油杉7个自然种群的遗传多样性进行分析。结果表明：用12条ISSR引物对218个黄枝油杉个体进行扩增，共扩增出125个位点。在物种水平上，多态性位点百分数（PPL）为100.00%，Shannon 信息多样性指数（I）为0.417 7，Nei’s基因多样性指数（H）为0.266 6。在种群水平上，多态性位点百分数（PPL）在71.20%~92.00%之间，平均值为80.69%，Shannon 信息多样性指数（I）在0.327 3~0.388 6之间，平均值为0.354 8，Nei’s基因多样性指数（H）在0.213 9~0.247 8之间，平均值为0.229 1；黄枝油杉在物种水平和种群水平均显示出较高的遗传多样性。Nei’s遗传多样性分析（Gst=0.143 3）和AMOVA分析（Φst=17.91%）表明，黄枝油杉的遗传变异主要存在于种群内，种群间的遗传分化程度较低；种群间保持一定的基因交流（Nm =2.989 0>1）。Mantel分析显示，黄枝油杉种群间的遗传距离和地理距离之间不存在显著的相关关系（r=0.456 7, P=0.061 0＞0.05）。

关键词: 黄枝油杉， ISSR分子标记，遗传多样性，遗传结构，保护策略

DOI：10.11931/guihaia.gxzw201606002

分类号:Q943

文章编号:10003142(2017)01003606

基金项目:广西科学研究与技术开发计划项目（桂科能1598025 49）；广西林业科技项目（桂林科字［2012］第26号和［2015］第26号）［Supported by Guangxi Science Research and Technology Development Program (1598025 49); Guangxi Forestry Science and Technology Program (［2012］ No. 26 and ［2015］ No. 26)］。

ISSR analysis on genetic diversity of Keteleeria calcarea

XIE WeiLing, CHAI ShengFeng*, JIANG YunSheng, TANG JianMin, CHEN ZongYou, ZOU Rong, WEI Xiao

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China

Abstract:

ISSR markers were used to investigate the genetic diversity of seven natural populations of Keteleeria calcarea. Twelve ISSR primers produced 125 loci. At the species level, the percentage of polymorphic loci (PPL) was 100.00%, Shannon’s Information index (I) was 0.417 7 and Nei’s gene diversity index (H) was 0.266 6. At the population level, the percentage of polymorphic loci was 71.20%-92.00% and the average value was 80.69%, Shannon’s Information index (I) was 0.327 3-0.388 6 with the average value 0.3548 and Nei’s gene diversity index (H) was 0.213 9-0.247 8 with the average value 0.229 1. K. calcarea in both species and population levels showed higher genetic diversity. Nei’s genetic diversity analysis (Gst=0.143 3) and AMOVA analysis (Φst=17.91%) showed that the genetic variation of K. calcarea mainly occurred within populations, and the genetic differentiation among populations was low. The gene flow (Nm) between populations was high (2.989 0). Mantel analysis showed that the genetic distance of K. calcarea natural populations had no significant correlation with their geographic distance (r=0.456 7, P=0.061 0＞0.05).

Key words: Keteleeria calcarea, ISSR markers, genetic diversity, genetic structure, conservation strategy