| 引用本文: | 候师师, 李 蜜, 姜 舒, 韩敏敏, 刘永宏, 易湘茜.海南西海岸四种真红树根系土壤放线菌物种多样性及其延缓衰老活性初筛[J].广西植物,2020,40(3):320-326.[点击复制] |
| HOU Shishi, LI Mi, JIANG Shu, HAN Minmin, LIU Yonghong, YI Xiangxi.Species diversity and anti-aging activity of actinobacteria from four true mangrove rhizosphere soils in the west coast of Hainan[J].Guihaia,2020,40(3):320-326.[点击复制] |
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| 海南西海岸四种真红树根系土壤放线菌物种多样性及其延缓衰老活性初筛 |
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候师师, 李 蜜, 姜 舒, 韩敏敏, 刘永宏, 易湘茜*
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广西中医药大学 海洋药物研究院/药学院, 南宁 530200
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| 摘要: |
| 为更好地开发利用海洋微生物资源积累丰富的放线菌菌种,该文以海南西海岸潮间带区域四种真红树根系土壤为研究对象,分析了红树林根系放线菌物种多样性组成及其代谢产物活性。该研究以9种不同培养基为分离介质,采用纯培养方法和三区划线法分离纯化菌株,结合放线菌形态学特征及其16S rRNA基因序列结果开展多样性分析。放线菌发酵液经乙酸乙酯萃取,利用秀丽隐杆线虫(Caenorhabditis elegans)测试其延缓衰老活性。结果表明:(1)共分离获得22株放线菌,隶属于4目7科9属,其中链霉菌属(Streptomyces)为优势菌群,并初步确认IMDGX 6012、IMDGX 6028、IMDGX 6118、IMDGX 6326、IMDGX 6119 5株放线菌可能为潜在的新物种。(2)发酵产物延缓衰老研究结果表明,8株放线菌的代谢产物可显著延长线虫寿命(P<0.05); 其中,IMDGX 6028和IMDGX 6118作为拟无枝酸菌属(Amycolatopsis)和短小杆菌属(Curtobacterium)的潜在新物种,具有极显著延缓衰老活性(P<0.01),与空白组比较,可分别延长线虫寿命的22.2%和26.6%。综上结果表明海南西海岸真红树根系土壤具有丰富的可培养放线菌资源,具有发现放线菌新物种和延缓衰老活性菌株的潜力。 |
| 关键词: 红树林, 根系放线菌, 菌种多样性, 延缓衰老活性 |
| DOI：10.11931/guihaia.gxzw201904051 |
| 分类号:Q939.1 |
| 文章编号:1000-3142(2020)03-0320-07 |
| 基金项目:国家自然科学基金(81903533, 41566004, 21662006); 广西自然基金面上项目(2018GXNSFAA281268); 广西中医药大学岐黄工程高层次人才团队培育项目(2018006); 广西中医药大学2017年引进博士科研启动基金(2017BS039); 广西中医药大学海洋药物研究院科研专项项目(2018ZD005-A06)[Supported by the National Natural Science Foundation(81903533, 41566004, 21662006); Guangxi General Program of Natural Science Foundation(2018GXNSFAA281268); Development Program of High-Level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(2018006); Research Launching Fund for Introduced Doctors from Guangxi University of Chinese Medicine in 2017(2017BS039); Special Fund of Marine Drugs Institute of Guangxi University of Chinese Medicine(2018ZD005-A06)]。 |
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| Species diversity and anti-aging activity of actinobacteria from four true mangrove rhizosphere soils in the west coast of Hainan |
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HOU Shishi, LI Mi, JIANG Shu, HAN Minmin, LIU Yonghong, YI Xiangxi*
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Institutes of Marine Drugs/Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
Institutes of Marine Drugs/Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
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| Abstract: |
| Four true mangrove rhizosphere soils in the intertidal zone of the west coast of Hainan were studied to analyze the species diversity composition and metabolite activity of mangrove rhizosphere actinomycetes, in order to accumulate abundant actinomycetes for better exploitation and utilization of marine microbial resources. In this study, seven samples of true mangrove rhizosphere soil were selected as research objects and nine different media were used as the isolation media. Pure culture method and three-zone scribing method were used to isolate and purify the strains. The species diversity composition was analyzed by combinating with the morphological characteristics of actinomycetes and the 16S rRNA gene sequence results. The actinomycete fermentation broth was extracted with ethyl acetate, and its anti-aging activity was tested using Caenorhabditis elegans model. The results were as follows:(1)A total of 22 strains of actinomycetes were isolated, belonging to four orders, seven families and nine genera consist of Amycolatopsis, Curtobacterium, Demequina, Isoptericola, Lysinimicrobium, Microbacterium, Rhodococcus, Sinomonas, Streptomyces, and Streptomyces was the dominant flora of this study with eleven strains isolated, and five strains of IMDGX 6012, IMDGX 6028, IMDGX 6118, IMDGX 6326, IMDGX 6119 were identified as potential new species actinobacteria. The highest similarities among the strains and the effective published strains Amycolatopsis lexingtonensis, A. niigatensis, Curtobacterium albidum, C. cit-reum, Demequina salsinemoris were 97.75%, 98.15%, 98.32%, 98.44%, 98.45%, respectively. The above five rhizosphere actinobacteria belong to rare actinobacteria and were preliminarily identified as potential new species.(2)The results of anti-aging of fermentation products showed that the metabolites of eight strains of actinobacteria could signifi-cantly prolong the lifespan of nematodes(P<0.05), including one strains of Curtobacterium, one strains of Demequina, one strain of Sinomonas and five of which were from Streptomyces, indicating that Streptomyces had potential to produce anti-aging active substances. IMDGX 6028 and IMDGX 6118, as potential new species of Amycolatopsis and Curtobacte-rium, had extremely significant anti-aging activity(P<0.01). When the crude metabolite concentration was 500 μg·mL-1, the survival time of C. elegans was increased by 22.2% and 26.6%, respectively, compared with the blank group. The results indicate that there are abundant resources of culturable actinobacteria and the potential to discover new species and anti-aging activity strains in the true mangrove rhizosphere soils in the west coast of Hainan. |
| Key words: mangrove plants, rhizosphere actinobacteria, species diversity, anti-aging activity |
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