实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数

王爱民<sup>1; 2</sup>

引用本文:	王爱民.实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数[J].广西植物,2009,(3):408-412.[点击复制]
	WANG Ai-Min.Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan)[J].Guihaia,2009,(3):408-412.[点击复制]

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实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数
王爱民^1,2
1.徐州师范大学生命科学学院, 江苏徐州 221116;2.江苏省药用植物生物技术重点实验室,江苏徐州 221116

摘要:

实时荧光定量PCR是近年新兴的一项技术,因其快速、方便、便宜,需要DNA样品量少,无需放射性检测等优点被广泛应用于基因的定量分析。该文就实时荧光定量PCR(TaqMan)技术的发展、基本原理及测定外源基因拷贝数的技术流程做一介绍。

关键词: 外源基因拷贝数实时荧光定量PCR

DOI：

分类号:Q943

基金项目:

Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan)

WANG Ai-Min^1,2

1.School of Life Science, Xuzhou Normal University, Xuzhou 221116, China;2.Jiangsu Provincial Key Laboratory of Biotechnology for Medicinal Plant, Xuzhou 221116, China 1.School of Life Science, Xuzhou Normal University, Xuzhou 221116, China; 2.Jiangsu Provincial Key Laboratory of Biotechnology for Medicinal Plant, Xuzhou 221116, China

Abstract:

Real-time fluorescent quantitative PCR is a new technique which has been developed to be used in quantitative analysis of genes as well with the advantages of simpleness,quickness,short needs of target fragment DNA and none of hazardous radioisotopes in recent years. The development,the principle of real-time fluorescent quantitative PCR(TaqMan)and the techinical flow of estimating copy number of transgenic gene are introuduced in this paper.

Key words: transgenic gene copy number real-time fluorescent quantitative PCR