忽地笑愈伤组织培养条件对加兰他敏合成的影响

茹巧美1; $21; 2; $21*

引用本文:	茹巧美1，裴真明1，2，郑海雷1*.忽地笑愈伤组织培养条件对加兰他敏合成的影响[J].广西植物,2010,(3):411-415.[点击复制]
	RU Qiao Mei1， PEI Zhen Ming1，2， ZHENG Hai Lei1*.Effects of the culture conditions on galanthamine biosynthesis in Lycoris aurea calluses[J].Guihaia,2010,(3):411-415.[点击复制]

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忽地笑愈伤组织培养条件对加兰他敏合成的影响
茹巧美1，裴真明1，2，郑海雷1*
1.厦门大学生命科学学院，福建厦门 361005;2.杜克大学生物系， NC 27708， USA

摘要:

研究了忽地笑次生代谢产物加兰他敏的合成以及各种理化因子对加兰他敏合成的影响。结果表明，蔗糖是忽地笑愈伤组织培养的最佳碳源，而且在90 g/L浓度培养下加兰他敏含量最高，达到0068%；附加500 mg/L苯〖JP2〗丙氨酸前体物也可提高加兰他敏产量。酪氨酸和水杨酸不利于加兰他敏的积累。此外，2，4 D和浓度高于0.5 mg/L的NAA、IBA抑制加兰他敏的合成，但高浓度的6 BA却〖JP〗对加兰他敏的合成起促进作用。

关键词: 忽地笑愈伤组织加兰他敏蔗糖 L 苯丙氨酸

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基金项目:

Effects of the culture conditions on galanthamine biosynthesis in Lycoris aurea calluses

RU QiaoMei1， PEI ZhenMing1，2， ZHENG HaiLei1*

1.School of Life Sciences， Xiamen University， Xiamen 361005， China;2.Department of Biology， Duke University， Durham， NC 27708， USA 1.School of Life Sciences， Xiamen University， Xiamen 361005， China； 2.Department of Biology， Duke University， Durham， NC 27708， USA

Abstract:

The biosynthesis of galanthamine in callus culture of Lycoris aurea was studied. Some factors affected the biosynthesis of galanthamine were investigated. The results showed that sucrose was the best carbon source，and the concentration of 90 g/L was the most preferable for galanthamine production，which was reached to 0.068 %. Meanwhile，adding 500 mg/L L phenylalanine to medium，galanthamine content was also improved. Tyrosine and salicylic acid were not suitable for galanthamine accumulation in calluses. Besides，2，4 D inhibited the biosynthesis of galanthamine. Moreover，NAA and IBA above 0.5 mg/L could restrain the production of galanthamine，but 6 BA treated with high concentration promoted the biosynthesis of galanthamine.

Key words: Lycoris aurea callus galanthamine sucrose L phenylalanine