| 引用本文: | 张泽宇, 李子院, 王少杨, 陈心怡, 汪利琪, 李海云.李氏禾内生细菌Bacillus cereus J01吸附Cr3+的研究[J].广西植物,2024,44(10):1839-1847.[点击复制] |
| ZHANG Zeyu, LI Ziyuan, WANG Shaoyang, CHEN Xinyi, WANG Liqi, LI Haiyun.Study on adsorption of chromium(Cr3+)by endophytic bacteria Bacillus cereus J01 isolatedfrom Leersia hexandra Swartz[J].Guihaia,2024,44(10):1839-1847.[点击复制] |
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| 李氏禾内生细菌Bacillus cereus J01吸附Cr3+的研究 |
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张泽宇, 李子院, 王少杨, 陈心怡, 汪利琪, 李海云*
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桂林理工大学 化学与生物工程学院, 广西高校食品安全与检测重点实验室, 广西 桂林 541004
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| 摘要: |
| 为考察李氏禾内生细菌蜡样芽孢杆菌Bacillus cereus J01菌株吸附Cr3+的性能,以蜡样芽孢杆菌Bacillus cereus J01失活菌体作为生物吸附剂,采用单因素实验方法考察pH、温度、Cr3+初始浓度、吸附剂用量、吸附时间等条件对Cr3+吸附性能的影响,分析其等温吸附过程、动力学过程及热力学过程,并采用红外光谱方法对吸附机理进行初步分析。结果表明:(1)在50 mL反应体系中,当pH值为6、温度为40 ℃、Cr3+初始浓度为150 mg·L-1、吸附剂投加量为0.2 g、吸附时间为12 h时,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr3+的吸附性能达到最佳,其平衡吸附量和Cr3+去除率分别为34.30 mg·g-1、91.60%。(2)等温吸附过程分析结果显示,朗格缪尔吸附等温模型能更好地模拟蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr3+的吸附,其吸附过程更倾向于单分子层吸附。(3)吸附过程动力学分析结果显示,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr3+的吸附更符合准二级动力学速率方程。(4)吸附过程热力学分析结果显示,在40 ℃下,吸附过程的△G、△H和△S分别为-2.609 kJ·mol-1、61.792 kJ·mol-1和206.11 J·mol-1,该温度下吸附过程是自发过程。(5)红外光谱分析结果显示,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr3+的吸附可能通过细胞成分中的氨基、羟基和羰基起作用。该研究结果表明该菌的失活菌体对Cr3+具有较强的吸附性能,并在铬污染的环境治理中具有较好的应用潜力。 |
| 关键词: 蜡样芽孢杆菌, 吸附, Cr3+, 李氏禾, 内生细菌 |
| DOI：10.11931/guihaia.gxzw202304007 |
| 分类号:Q946 |
| 文章编号:1000-3142(2024)10-1839-09 |
| 基金项目:广西自然科学基金(2016GXNSFAA380014); 国家自然科学基金(31460409)。 |
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| Study on adsorption of chromium(Cr3+)by endophytic bacteria Bacillus cereus J01 isolatedfrom Leersia hexandra Swartz |
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ZHANG Zeyu, LI Ziyuan, WANG Shaoyang, CHEN Xinyi, WANG Liqi, LI Haiyun*
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Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry
and Bioengineering, Guilin University of Technology, Guilin 541004, Guangxi, China
Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry
and Bioengineering, Guilin University of Technology, Guilin 541004, Guangxi, China
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| Abstract: |
| The effects of pH, temperature, initial Cr3+ concentration, adsorbent dosage, time, and other factors on the adsorption capacity of Cr3+ were examined by the single factor test method using inactivated Bacillus cereus J01 strain as biological adsorbent in order to investigate the adsorption performance of Cr3+ by endophytic bacterium Bacillus cereus J01 from Leersia hexandra Swartz. The kinetic, thermodynamic, and isothermal adsorption processes were analyzed. Infrared spectroscopy was used to provide a preliminary analysis of the Cr3+ adsorption mechanism. The results were as follows:(1)In the reaction system of 50 mL, inactivated Bacillus cereus J01 reached the best adsorption performance on Cr3+ when pH value was 6, temperature was 40 ℃, initial concentration of Cr3+ was 150 mg·L-1, adsorbent dosage was 0.2 g and adsorption time was 12 h. The equilibrium adsorption capacity was 34.30 mg·g-1 and the removal rate of Cr3+ was 91.60%.(2)The Langmuir isothermal adsorption model could better simulate the adsorption of Cr3+ by inactivated Bacillus cereus J01. The adsorption process was more similar to monolayer adsorption.(3)The adsorption kinetics analysis showed that the adsorption of inactivated Bacillus cereus J01 on Cr3+ was more consistent with quasi-second-order kinetic rate equation.(4)Thermodynamic analysis of adsorption showed that at 40 ℃, △G, △H and △S were -2.609 kJ·mol-1, 61.792 kJ·mol-1 and 206.11 J·mol-1, respectively. The adsorption process was spontaneous at 40 ℃.(5)The results of infrared spectroscopy showed that the adsorption of inactivated Bacillus cereus J01 on Cr3+ could be attributed to the effects of amino, hydroxyl and carbonyl groups in the cell components. The results show that the inactivated bacteria of this bacterium has strong adsorption capacity for Cr3+, and has good application potential in the treatment of environmental chromium pollution. |
| Key words: Bacillus cereus, adsorption, Cr3+, Leersia hexandra, endophytic bacterium |
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