| 引用本文: | 李连春, 符毓夏, 谭兰芳, 宁德生, 潘争红.地枫皮入血成分抗类风湿性关节炎作用研究[J].广西植物,2025,45(3):517-526.[点击复制] |
| LI Lianchun, FU Yuxia, TAN Lanfang, NING Desheng, PAN Zhenghong.Anti-rheumatoid arthritis effect of entering bloodstream components from Illicium difengpi[J].Guihaia,2025,45(3):517-526.[点击复制] |
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| 地枫皮入血成分抗类风湿性关节炎作用研究 |
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李连春, 符毓夏, 谭兰芳, 宁德生, 潘争红*
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广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质与资源持续利用重点实验室, 广西 桂林 541006
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| 摘要: |
| 为发现地枫皮中具有抗类风湿性关节炎(anti-rheumatoid arthritis,anti-RA)功效的化合物,该研究利用液相色谱-串联质谱(LC-MS/MS)法鉴定地枫皮的入血成分,通过检测入血成分对胶原诱导的关节炎小鼠表观病症、关节炎指数、膝关节病理变化,以及对血清中MCP-1、IL-6和TNF-α含量的影响,评价入血成分的抗RA活性,同时利用分子对接的方法,探究抗RA活性化合物与TLR4/NF-κB通路的相互作用。结果表明:(1)地枫皮中的异红花八角醇和厚朴酚被吸收后能够进入血浆。(2)异红花八角醇能够缓解胶原诱导关节炎小鼠的四肢充血、发红、肿胀等症状,减小四肢关节炎指数打分,阻碍膝关节组织病理进程,降低血清中MCP-1、IL-6和TNF-α的含量。(3)异红花八角醇能够与TLR4/NF-κB通路中的关键蛋白质TLR4、IκBα和NF-κB p65结合。综上认为,异红花八角醇被吸收后,通过血液循环到达关节组织,可能通过影响TLR4/NF-κB通路而缓解RA病症,是地枫皮抗RA的活性成分之一。 |
| 关键词: 地枫皮, 异红花八角醇, 类风湿性关节炎, 入血, 液相色谱-串联质谱法, TLR4/NF-κB通路 |
| DOI：10.11931/guihaia.gxzw202310044 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)03-0517-10 |
| 基金项目:国家自然科学基金(31960091); 广西自然科学基金(2019GXNSFAA245060); 桂林市创新平台和人才计划(20210102-3)。 |
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| Anti-rheumatoid arthritis effect of entering bloodstream components from Illicium difengpi |
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LI Lianchun, FU Yuxia, TAN Lanfang, NING Desheng, PAN Zhenghong*
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Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany,
Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany,
Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| To search the compounds with anti-rheumatoid arthritis(anti-RA)efficacy from Illicium difengpi, the components that can enter bloodstream were identified by liquid chromatography-tandem mass spectrometry(LC-MS/MS), and then their anti-RA activities were evaluated by testing their effects on symptoms, arthritis indexes, pathological changes of knee joints, and contents of MCP-1, IL-6 and TNF-α in serum of collagen-induced arthritis mice. The interactions between the anti-RA active compounds and TLR4/NF-κB pathway were studied by molecular docking. The results were as follows:(1)After absorbance, isodunnianol and magnolol of I. difengpi could enter bloodstream.(2)Isodunnianol could alleviate symptoms including limbs congestion, redness and swelling, reduce arthritis index score of limbs, hinder pathological process of knee joints tissue, and decrease contents of MCP-1, IL-6 and TNF-α in serum of collagen-induced arthritis mice.(3)Isodunnianol could bind to key proteins of TLR4/NF-κB pathway, including TLR4, IκBα and NF-κB p65. In conclusion, isodunnianol can reach the joint tissue through the bloodstream after being absorbed and can possibly alleviate the pathology of RA through regulating TLR4/NF-κB pathway. Isodunnianol is one of anti-RA active components of I. difengpi. |
| Key words: Illicium difengpi, isodunnianol, rheumatoid arthritis(RA), enter bloodstream, LC-MS/MS, TLR4/NF-κB pathway |
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