| 引用本文: | 但金龙, 李海云, 王亚凤, 阳丙媛, 李桂勤, 何瑞杰, 黄永林.湄公锥的化学成分及其α-葡萄糖苷酶抑制活性研究[J].广西植物,2025,45(10):1764-1773.[点击复制] |
| DAN Jinlong, LI Haiyun, WANG Yafeng, YANG Bingyuan,
LI Guiqin, HE Ruijie, HUANG Yonglin.Chemical constituents of Castanopsis mekongensis and their α-glucosidase inhibitory activity[J].Guihaia,2025,45(10):1764-1773.[点击复制] |
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| 湄公锥的化学成分及其α-葡萄糖苷酶抑制活性研究 |
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但金龙1,2, 李海云1, 王亚凤2, 阳丙媛2, 李桂勤2, 何瑞杰2, 黄永林2*
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1.桂林理工大学 化学与生物工程学院, 广西 桂林 541006;2. 广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质与资源持续利用重点实验室, 广西 桂林 541006
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| 摘要: |
| 为探究湄公锥(Castanopsis mekongensis)叶的化学成分及其α-葡萄糖苷酶抑制活性,该研究以湄公锥叶70%甲醇提取物为研究对象,选用多种现代分离材料对其开展系统的分离纯化,综合运用NMR、MS等波谱分析和文献比较法鉴定化合物结构,并对分离得到的化合物使用pNPG法筛选其降糖活性。结果表明:(1)从湄公锥叶中分离出17个化合物,分别鉴定为湄公锥A(1)、没食子酸(2)、3,4二羟基-苯甲酸(3)、丁香酸(4)、3,4-二羟基苯甲酸甲酯(5)、没食子酸甲酯(6)、鞣花酸(7)、龙胆酸5-O-β-D-木糖苷(8)、3-O-没食子酰基莽草酸(9)、2,3-O-(S)-六羟基二苯基-吡喃葡萄糖(10)、casuariin(11)、5-desgalloylstarchyurin(12)、芦丁(13)、山奈酚3-O-芸香糖苷(14)、槲皮素-3-O-(6″-O-没食子酰基-β-D-吡喃葡萄糖苷)(15)、槲皮素-3-O-(6″-O-没食子酰基-β-D-吡喃半乳糖苷)(16)、quercetin-3-O-β-D-glucopyranoside(3'→O-3″)quercetin-3-O-β-D-galactopyranoside(17)。其中,化合物1为新化合物,所有化合物均为首次从湄公锥植物中分离得到。(2)药理试验结果显示,化合物3、9-12、14、17比阳性对照阿卡波糖表现出更强的α-葡萄糖苷酶抑制活性。该研究结果丰富了湄公锥的化学成分,明确了其降糖活性成分,为后续降糖相关产品开发提供了理论依据。 |
| 关键词: 湄公锥, 化学成分, α-葡萄糖苷酶, 湄公锥A, 没食子酸 |
| DOI：10.11931/guihaia.gxzw202402036 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)10-1764-10 |
| 基金项目:国家自然科学基金(82060764, 32060108); 广西科学院基本科研业务费项目(CQZ-C-1901); 桂林市创新平台与人才计划项目(20210102-3); 广西植物功能物质与资源持续利用重点实验室主任项目(ZRJJ2023-8) |
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| Chemical constituents of Castanopsis mekongensis and their α-glucosidase inhibitory activity |
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DAN Jinlong1,2, LI Haiyun1, WANG Yafeng2, YANG Bingyuan2,
LI Guiqin2, HE Ruijie2, HUANG Yonglin2*
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1.College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, Guangxi, China;2. Guangxi Key Laboratory
of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang
Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| Castanopsis mekongensis is mainly distributed in southern and southwestern Yunnan, China, growing in mountainous areas below an altitude of about 2 000 m. This plant is also widely distributed in Laos. According to folk records, C. mekongensis has medicinal effects of clearing heat and detoxifying, nourishing the stomach and strengthening the spleen. In order to explore the chemical constituents of C. mekongensis leaves and their α-glucosidase inhibitory activity, various modern chromatographic separation techniques were used to separate and purify 70% methanol extract from leaves of C. mekongensis. The structures of all compounds were determined by comprehensive analysis methods, including mass spectrometry(MS), nuclear magnetic resonance spectroscopy(NMR), and literature comparison. At the same time, the compounds were screened by testing for α-glucosidase inhibitory activity using the pNPG method. The results were as follows:(1)Seventeen compounds were isolated from the leaves of C. mekongensis, which were identified as mekongensis A(1), gallic acid(2), 3,4-dihydroxybenzoic acid(3), syringate(4), protocatechuic acid methyl ester(5), methyl gallate(6), ellagic acid(7), gentisic acid 5-O-β-D-xyloside(8), 3-O-gallic acid(9), 2,3-O-(S)-hexahydroxydiphenyl-β-D-glucopyranose(10), casuariin(11), 5-degalloylstarchyurin(12), rutin(13), kaempferol 3-O-rutoside(14), quercetin-3-O-(6″-O-galloyl)-β-D-glucopyranoside(15), quercetin-3-O-(6″-O-galloyl)-β-D-galactopyranoside(16), and quercetin-3-O-β-D-glucopyranoside(3'→O-3″)quercetin-3-O-β-D-galactopyranoside(17). Compound 1 is a new compound, and all compounds were isolated for the first time from C. mekongensis. (2)Pharmacological experimental results showed that compounds 3, 9-12, 14, 17 had strong α-glucosidase inhibitory activity, with IC50 values of(0.36±0.053)mmol·L-1,(0.03±0.398)mmol·L-1,(0.24±0.035)mmol·L-1,(0.41±0.283)mmol·L-1,(0.18±0.154)mmol·L-1,(0.15±0.296)mmol·L-1 and(0.22±0.095)mmol·L-1 [acarbose as the positive control, with IC50 value of(0.91±0.226)mmol·L-1]. Compounds 3, 9-12, 14, 17 are considered potential candidates for developing new antidiabetic drugs based on their performance in biological activity assays. The research results have enriched the chemical constituents of C. mekongensis, further clarified its hypoglycemic active ingredients, and provided a theoretical foundation for further development of hypoglycemic related products. |
| Key words: Castanopsis mekongensis, chemical constituents, α-glucosidase, mekongensis A, gallic acid |
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