| 引用本文: | 王 婷, 刘含颖, 明安刚, 滕金倩, 张 敬, 谢 婷, 覃 林.马尾松与格木混交林及其纯林土壤碳水化合物活性酶基因分布特征[J].广西植物,2025,45(10):1901-1915.[点击复制] |
| WANG Ting, LIU Hanying, MING Angang, TENG Jinqian,
ZHANG Jing, XIE Ting, QIN Lin.Distribution characteristics of soil CAZymes genes in Pinus massoniana and Erythrophleum fordii mixed plantations and their pure stands[J].Guihaia,2025,45(10):1901-1915.[点击复制] |
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| 马尾松与格木混交林及其纯林土壤碳水化合物活性酶基因分布特征 |
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王 婷1, 刘含颖1, 明安刚2,3, 滕金倩2, 张 敬1, 谢 婷1, 覃 林1*
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1.广西大学 林学院, 广西森林生态与保育重点实验室, 南宁 530004;2. 中国林业科学研究院热带林业
实验中心, 广西 凭祥 532600;3. 广西友谊关森林生态系统定位观测研究站, 广西 凭祥 532600
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| 摘要: |
| 为研究降解土壤不同来源(植物、微生物)成分的碳水化合物活性酶(CAZymes)基因丰度,对针叶林改造成阔叶林或针阔混交林后的响应,该文对南亚热带马尾松林、格木林、马尾松-格木混交林不同土壤深度(0～20 cm、20～40 cm、40～60 cm)的宏基因组数据进行分析,结合土壤理化性质,探究降解植物和微生物成分的CAZymes基因丰度对不同林分不同土壤深度的响应,并分析其主导调控因子。结果表明:(1)林分改造后,降解植物成分的CAZymes基因(GH116、GH115和AA5)丰度显著提高,与土壤有机碳(SOC)、C/N和微生物生物量碳(MBC)含量显著相关。(2)马尾松-格木混交林中GH102、GH25基因具有显著优势,表明针阔混交林中降解微生物成分的能力相较其他2个林分更强,3个林分土壤中降解植物和微生物成分的CAZymes基因丰度基本随土层深度增加而降低,主要与SOC含量的显著正效应有关。(3)随着3个林分生长至后期,微生物源碳对SOC的贡献可能逐渐大于植物源碳,并且细菌源成分贡献最大。综上认为,CAZymes基因分布与SOC含量密切相关,与纯林相比,马尾松-格木混交林中的GH102、GH25基因优势显著,其碳储存潜力可能更高。该研究结果对评估人工林生态系统的土壤碳储存潜力具有一定指导意义。 |
| 关键词: 碳水化合物活性酶, 宏基因组测序, 碳储存, 针阔人工混交林, 土壤有机碳 |
| DOI：10.11931/guihaia.gxzw202410011 |
| 分类号:Q948 |
| 文章编号:1000-3142(2025)10-1901-15 |
| 基金项目:国家自然科学基金(31560109, 32071764); 广西自然科学基金(2020GXNSFAA297208) |
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| Distribution characteristics of soil CAZymes genes in Pinus massoniana and Erythrophleum fordii mixed plantations and their pure stands |
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WANG Ting1, LIU Hanying1, MING Angang2,3, TENG Jinqian2,
ZHANG Jing1, XIE Ting1, QIN Lin1*
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1.Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China;2. Experiment Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, Guangxi, China;3. Guangxi Youyiguan Forest Ecosystem Research Station, Pingxiang 532600, Guangxi, China
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| Abstract: |
| To study the gene abundance of carbohydrate-active enzymes(CAZymes)that degrade components from different soil sources(plant and microorganisms)provides certain guidance for assessing the soil carbon storage potential of plantation ecosystems for the response of forests after they are tranformed into to broad-leaved or mixed coniferous-broad-leaved plantations. In this study, metagenomic data of different soil depths(0-20 cm, 20-40 cm, 40-60 cm)of Pinus massoniana forest, Erythrophleum fordii and Pinus massoniana × Erythrophleum fordii mixed plantations in south subtropical China were analyzed, and combined with soil physicochemical properties, the response of gene abundance of CAZymes involved in degrading plant and microbial components to different stands and different soil depths was investigated. Meanwhile, the main regulatory factors were analyzed. The results were as follows:(1)After forest conversion, the gene abundance of CAZymes(GH116, GH115, and AA5)that degraded plant components was significantly increased and was strongly correlated with soil organic carbon(SOC), C/N ratio, and microbial biomass carbon(MBC)content.(2)The significant abundance of GH102 and GH25 genes in Pinus massoniana × Erythrophleum fordii mixed plantations indicated that the ability to degrade microbial components in mixed coniferous-broad-leaved plantations was stronger compared to the other two stands. The gene abundance of CAZymes that degraded plant and microbial components in soils of the three stands generally decreased with increasing soil depth, primarily due to the significant positive effect of SOC content.(3)With the growth of the three stands to the later stage, the contribution of microbial-derived carbon to SOC may gradually exceed that of plant-derived carbon, with bacterial-derived components contributing the most. Overall, the distribution of CAZymes genes is closely related to SOC content. Compared to pure stands, Pinus massoniana × Erythrophleum fordii mixed plantations show significant advantages in the gene abundance of GH102 and GH25, potentially indicating higher carbon storage potential. The research results have certain guiding significance for assessing the soil carbon storage pitential of plantations. |
| Key words: carbohydrate-active enzymes(CAZymes), metagenomic sequencing, carbon storage, mixed coniferous-broad-leaved plantation, soil organic carbon |
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