| 引用本文: | 郁培义, 许 强, 周 宵, 张 超, 潘 登.菌根化马尾松转录组差异分析[J].广西植物,2025,45(10):1936-1948.[点击复制] |
| YU Peiyi, XU Qiang, ZHOU Xiao, ZHANG Chao, PAN Deng.Difference analysis on transcriptome sequencing of Pinus massoniana inoculated with ectomycorrhizal fungi[J].Guihaia,2025,45(10):1936-1948.[点击复制] |
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| 菌根化马尾松转录组差异分析 |
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郁培义1, 许 强1, 周 宵2, 张 超3, 潘 登3*
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1.山西电子科技学院, 山西 临汾 041000;2. 湖南省林业科学院省部共建木本油料资源利用国家
重点实验室, 长沙 410004;3. 国家林业和草原局中南调查规划院, 长沙 410014
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| 摘要: |
| 外生菌根菌可以促进宿主植物在胁迫环境下的生长及生理活性,而这个过程往往依赖于共生系统复杂的分子调控模式。为了更好地研究外生菌根菌对马尾松生长适应的分子机制,该研究以未接种条件下的马尾松为对照组,测定菌根化马尾松幼苗生长量、叶绿素含量及抗氧化酶含量,利用转录组技术手段比较分析了接种处理和未接种处理之间差异表达基因,并对差异表达基因进行功能注释和代谢通路分析。结果表明:(1)与未接种外生菌根菌马尾松相比,菌根化马尾松地上部分生物量及根长显著提高(P<0.05),叶绿素a、叶绿素b及过氧化氢酶(CAT)活性、过氧化物酶(POD)活性等生理指标也显著增强(P<0.05)。(2)共获得104 467 条Unigenes,Nr 和GO数据库的Unigene 数量最多,分别为66 641(63.79%)和57 483(55.03%),KOG 数据库的Unigene数量最少,占总量的11.71%。(3)共筛选到2 520个基因差异表达显著,与未接种处理相比,接菌组1 611个基因上调表达,909个基因下调表达。(4)GO 注释及KEGG 通路富集分析结果显示,大多数差异表达基因集中在能量代谢、次生代谢物生物合成及谷胱甘肽代谢途径等信号通路上,参与了马尾松的菌根形成、宿主植物的生长发育、细胞光合色素合成等,对于维持高水平的抗氧化物具有积极作用。以上结果揭示了接种外生菌根菌对马尾松之间的互作机制,探究了褐环乳牛肝菌对马尾松菌根形成过程中相关代谢通路及关键酶基因的影响,为后期开展马尾松菌根共生抗性基因筛选奠定了基础。 |
| 关键词: 马尾松, 外生菌根菌, 转录组, 差异表达基因, 代谢通路 |
| DOI：10.11931/guihaia.gxzw202411009 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)10-1936-13 |
| 基金项目:湖南省重大科技创新平台项目(2023PT1001)。 |
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| Difference analysis on transcriptome sequencing of Pinus massoniana inoculated with ectomycorrhizal fungi |
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YU Peiyi1, XU Qiang1, ZHOU Xiao2, ZHANG Chao3, PAN Deng3*
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1.Shanxi University of Electronic Science and Technology, Linfen 041000, Shanxi, China;2. State Key Laboratory of Utilization
of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China;3. Central South Academy
of Inventory and Planning of NFGA, Changsha 410014, China
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| Abstract: |
| Ectomycorrhizal fungi promote the growth and physiological activity of host plants under the stress conditions, however, this process often relies on complex molecular regulatory patterns of symbiotic systems. To better understand the molecular mechanism of the growth adaptation of Pinus massoniana to ectomycorrhizal fungi(Suillus luteus), this study used P. massoniana seedlings uninoculated with ectomycorrhizal fungi(NE)as the control group(CK), the growth, chlorophyll content and antioxidant enzyme content of mycorrhizal P. massoniana seedlings, and compared and analyzed the differentially expressed genes(DEGs)between inoculated(IN)and uninoculated(CK)treatments using transcriptome technology, followed by functional annotation and metabolic pathway analysis of the DEGs. The results were as follows:(1)Compared with NE, the aboveground biomass and root length of mycorrhizal P. massoniana inoculation were significantly increased(P<0.05), and the physiological indicators such as chlorophyll a, chlorophyll b, catalase(CAT)and peroxidase(POD)were also significantly enhanced(P<0.05).(2)A total of 104 467 Unigenes were obtained through Trinity software assembly, with the largest number of Unigenes in the Nr [66 641(63.79%)] and GO [57 483(55.03%)] databases, while the number of Unigenes in the KOG database was the lowest, accounting for only [12 233(11.71%)] of the total.(3)A total of 2 520 genes showed significant differential expression, compared with NE treatment, 1 611 genes were up-regulated and 909 genes were down-regulated in the IN group.(4)GO annotation and KEGG pathway enrichment analysis results showed that most differentially expressed genes were concentrated in energy metabolism, secondary metabolite biosynthesis, and glutathione metabolic pathways, and were involved in mycorrhiza formation, growth and development of the host plant, synthesis of cellular photosynthetic pigments, etc., playing a positive role in maintaining high levels of antioxidants. These results reveal the interaction mechanism between ectomycorrhizal fungi inoculation and P. massoniana, investigate the effects of Suillus luteus on related metabolic pathways and key enzyme genes during the mycorrhiza formation process of P. massoniana, and lay a foundation for future screening of resistance genes involved in mycorrhizal symbiosis in P. massoniana. |
| Key words: Pinus massoniana, ectomycorrhizal fungi, transcriptomes, differentially expressed genes, metabolic pathway |
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