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| 采用液质联用技术定性分析松叶鸡蛋参根的化学成分 |
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赵文慧1,2, 窦志扬2, 强 巴3*, 吾 坚3, 白玛卓玛3, 刘广学2, 蔡少青2, 徐 风1,2*
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1. 西藏大学 医学院,拉萨 850000;2. 北京大学 药学院, 北京 100191;3. 西藏奇正藏药股份有限公司, 西藏 林芝 860000
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| 摘要: |
| 为阐明藏药鸡蛋参 [植物松叶鸡蛋参(Codonopsis convolvulacea var. pinifolia)的根]的化学成分,该文采用超高效液相色谱-四级杆飞行时间质谱联用(UHPLC-Q-TOF-MS/MS)技术对其化学成分进行定性分析。采用Waters ACQUITY UPLC BEH C18(1.7 μm,2.1 mm × 150 mm)色谱柱,以0.1 mmol·L-1乙酸铵水溶液(A)-乙腈(B)为流动相,流速0.3 mL·min-1,进样体积3 μL,柱温40 ℃。质谱使用ESI离子源,采用负离子检测模式,扫描范围为m/z 100～1 800(MS)、m/z 50～1 800(MS/MS)。根据化合物一级质谱中的准分子离子推测其分子式,根据化合物二级质谱中的特征碎片离子推测其可能的结构片段及分子结构,再结合对照品对比和文献检索,进行结构鉴定。结果表明:(1)首次从松叶鸡蛋参中鉴定出56种化合物,其中含氮类成分6种、苯丙素类成分6种、木脂素类成分22种、黄酮类成分2种、有机酸类成分8种、糖苷或其他类成分12种; 采用对照品比对指认了其中11种成分。(2)首次发现木脂素类和苯丙素类化合物为松叶鸡蛋参根的主要成分,并推导出其裂解途径。该研究结果表明,应用UHPLC-Q-TOF-MS/MS技术能够快速高效地初步阐明松叶鸡蛋参根的化学成分,为藏药鸡蛋参的质量标准、体内过程及药效物质等研究提供了化学基础。 |
| 关键词: 松叶鸡蛋参, UHPLC-Q-TOF-MS/MS, 化学成分, 结构解析, 裂解途径 |
| DOI：10.11931/guihaia.gxzw202409042 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)03-0585-21 |
| Fund project:西藏自治区2024年度科技计划科技重大专项; 林芝市2022年度“区域科技协同创新”专项。 |
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| Qualitative analysis of chemical constituents of Codonopsis convolvulacea var. pinifolia roots by using LC-MS |
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ZHAO Wenhui1, 2, DOU Zhiyang2, Qiangba3*, Wujian3, Baimazhuoma3,
LIU Guangxue2, CAI Shaoqing2, XU Feng<sup>1,2*
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1. Medical College, Tibet University, Lhasa 850000, China;2. School of Pharmaceutical Sciences, Peking University,
Beijing 100191, China;3. Tibet Qizheng Tibetan Medicine Co. Ltd., Nyingchi 860000, Xizang, China
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| Abstract: |
| To clarify the chemical constituents of the Tibetan medicine Codonopsis Convolvulaceae Radix(the roots of Codonopsis convolvulacea var. pinifolia)by ultra high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UHPLC-Q-TOF-MS/MS), Waters ACQUITY UPLC BEH C18(1.7 μm, 2.1 mm × 150 mm)chromatographic column was used. The mobile phase was 0.1 mmol·L-1 ammonium acetate aqueous solution and acetonitrile. The flow rate was 0.3 mL·min-1, the injection volume was 3 μL, and the column temperature was 40 ℃. Electrospray ionization(ESI)source was used for mass spectrometry, and the detection was conducted in negative ion mode. The mass spectrometry scanning range was m/z 100-1 800(MS)and m/z 50-1 800(MS/MS). The molecular formula of a compound was deduced based on quasi-molecular ions in its first-order mass spectra, and its structural fragments and possible molecular structure were elucidated by analyzing the characteristic fragment ions in its second-order mass spectra. Finally, its structure was indentified by comparison with reference material and literature retrieval. The results were as follows:(1)Totally 56 compounds were tentatively identified from the roots of C. convolvulacea var. pinifolia for the first time, including 6 nitrogen-containing compounds, 6 phenylpropanoids, 22 lignans, 2 flavonoids, 8 organic acids, and 12 glucosides or other compounds. Among them, 11 compounds were unequivocally identified by comparison with reference compounds.(2)Lignans and phenylpropanoids were firstly found to be the main constituents of C. convolvulacea var. pinifolia roots, and their fragmentation pathways were deduced. In this study, the application of UHPLC-Q-TOF-MS/MS can quickly and efficiently elucidate chemical constituents of C. convolvulacea var. pinifolia roots. It provides a chemical basis for further studies of quality standard, in vivo process and pharmacodynamic substances of Codonopsis Convolvulaceae Radix. |
| Key words: Codonopsis convolvulacea var. pinifolia, UHPLC-Q-TOF-MS/MS, chemical constituents, structural elucidation, fragmentation pathway |
