| 引用本文: | 姜 舒, 李 蜜, 候师师, 韩敏敏, 银江林, 高程海.海南西海岸真红树内生放线菌多样性及其延缓衰老活性初筛[J].广西植物,2020,40(3):327-334.[点击复制] |
| JIANG Shu, LI Mi, HOU Shishi, HAN Minmin, YIN Jianglin, GAOChenghai.Diversity and anti-aging activity of endophytic actinobacteria from true mangrove plants collected from the west coast of Hainan[J].Guihaia,2020,40(3):327-334.[点击复制] |
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| 海南西海岸真红树内生放线菌多样性及其延缓衰老活性初筛 |
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姜 舒, 李 蜜, 候师师, 韩敏敏, 银江林, 高程海*
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广西中医药大学 海洋药物研究院/药学院, 南宁 530200
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| 摘要: |
| 为了发掘红树林内生放线菌资源和进行新型海洋药物研究,该文选择海南西海岸14种真红树的根、茎、叶、花、胚轴为研究对象,采用9种不同分离培养基[改良的高氏培养基(AGG)、海藻糖-天冬酰胺培养基(M4)、海藻糖-脯氨酸培养基(M5)、改良ISP5培养基(M7)、精氨酸-天冬酰胺培养基(M9)、改良淀粉-水解酪素培养基(M10)、酪氨酸-天冬酰胺培养基(P7)、燕麦培养基(P3)、棉籽糖-组氨酸培养基(M11)],结合稀释涂布法和三线划线法从真红树组织中分离菌株,基于菌株16S rRNA基因序列信息等分子生物学鉴定方法对获得的海洋放线菌进行多样性分析,并使用模式生物秀丽隐杆线虫对所得内生放线菌的延缓衰老活性进行研究。结果表明:(1)从46份真红树组织中共获得24株放线菌,隶属于7科11属,其中9株菌株为链霉菌属(Streptomyces),且IMDGX 6270、IMDGX 6137和IMDGX 6173为3株疑似潜在新菌株。(2)秀丽隐杆线虫模型筛选发现4株放线菌(IMDGX 6157、IMDGX 6182、IMDGX 6248、IMDGX 6360)具有延缓衰老的活性,与空白组比较,生存时间分别延长17.16%、28.11%、29.05%、27.10%。综上结果说明海南西海岸区域真红树植物中可能含有较丰富的放线菌资源,能够为延缓衰老药物的研发提供新来源。 |
| 关键词: 物种多样性, 内生放线菌, 真红树, 延缓衰老活性 |
| DOI：10.11931/guihaia.gxzw201904036 |
| 分类号:Q939.1 |
| 文章编号:1000-3142(2020)03-0327-08 |
| 基金项目:国家自然科学基金(41566004)[Supported by the National Natural Science Foundation of China(41566004)]。 |
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| Diversity and anti-aging activity of endophytic actinobacteria from true mangrove plants collected from the west coast of Hainan |
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JIANG Shu, LI Mi, HOU Shishi, HAN Minmin, YIN Jianglin, GAOChenghai*
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Institutes of Marine Drugs /College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
Institutes of Marine Drugs /College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
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| Abstract: |
| In order to find endophytic actinomycetes resources and research on new marine drugs, we studied the diversity and anti-aging activity of endophytic actinobacteria from ture mangrove plants of the west coast of Hainan. In this study, roots, stems, leaves, flowers and hypocotyl of 14 species of true mangrove plants from the west coast of Hainan were selected as research objects. Twenty-four strains of actinobacteria were obtained, from 46 plant tissues of ture mangrove plants, by using improved Gause's medium(AGG), trehalose-asparagine medium(M4), trehalose-proline medium(M5), improved ISP5 medium(M7), arginine-asparagine medium(M9), improved starch-hydrolyzed casein medium(M10), tyrosine-asparagine medium(P7), oat medium(P3), cottonseed sugar-histidine medium(M11)of nine kinds of different media, through dilution coating method and the third line marking method. The diversity of marine actinobacteria was analyzed by molecular biological identification methods such as 16S rRNA gene sequence information. The anti-aging activities of endophtic actinobacteria were analyzed by using Caenorhabditis elegans screening models. The results were as follows:(1)A total of 24 strains of actinobacteria were isolated from 46 plant tissues of true mangrove plants and distributed in eleven genera, seven families. Nine strains belonged to Streptomyces. Moreover, IMDGX 6270, IMDGX 6137 and IMDGX 6173 were three suspected potential new strains. The number of strains isolated from each sample varied greatly. Among them, the leaves had the largest number of strains and the richest diversity(18 strains). Next, eight strains were obtained from the stem. The number of roots was four, which may be related to the large number of stem and leaf tissue samples, and the number and diversity of strains isolated from M5 medium were the highest, while the number and diversity of strains isolated from M11 medium were the lowest. The results showed that the isolation medium with different nutrients had a great influence on the growth of microorganisms.(2)In addition, four strains of actinobacteria were screened by Caenorhabditis elegans model and showed anti-aging activity, and the survival time was increased by 17.16%, 28.11%, 29.05% and 27.10%, respectively, compared with the blank group. This indicates that there may be abundant actinobacteria resources in the mangrove plants in the west coast of Hainan, which can provide a new source for the research and development of anti-aging drugs. |
| Key words: species diversity, endophytic actinobacteria, true mangrove plants, anti-aging activity |
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