睫毛萼凤仙花的组织培养及其愈伤组织的超微观察

王雪娟<sup>1</sup>; 程新利<sup>2</sup>; 柴瑞娟<sup>3</sup>

引用本文:	王雪娟, 程新利, 柴瑞娟.睫毛萼凤仙花的组织培养及其愈伤组织的超微观察[J].广西植物,2011,(2):262-265.[点击复制]
	WANG Xue-Juan, CHENG Xin-Li, CHAI Rui-Juan.Tissue culture of Impatiens blepharosepala and ultrastructural observation of callus[J].Guihaia,2011,(2):262-265.[点击复制]

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睫毛萼凤仙花的组织培养及其愈伤组织的超微观察
王雪娟¹, 程新利², 柴瑞娟³
1.安徽科技学院城建与环境学院, 安徽凤阳 233100;2.天津市园林开发建设有限公司, 天津 300203;3.安徽工程科技学院生化工程系, 安徽芜湖 241000

摘要:

试验以睫毛萼凤仙花无菌苗的叶片、茎和根为外植体进行离体培养,并对两种具有不同再分化能力的愈伤组织进行电镜超微观察。结果表明:在培养基MS+NAA 0.5 mg/L+6-BA 0.5 mg/L上愈伤组织诱导率达最高,叶片愈伤组织的诱导率为100%,茎的愈伤组织诱导率为67%,而根却没有愈伤组织的发生; 叶片愈伤组织在MS+NAA 0.5 mg/L+6-BA 2.0 mg/L上培养40 d后有不定芽的发生,而茎的愈伤组织却没有器官发生; 电镜观察表明,具有再分化能力的愈伤组织的细胞排列不规则,其表面有较明显的类似组织分化。

关键词: 睫毛萼凤仙花离体培养超微观察

DOI：10.3969/j.issn.1000-3142.2011.03.023

分类号:Q813.1

基金项目:

Tissue culture of Impatiens blepharosepala and ultrastructural observation of callus

WANG Xue-Juan¹, CHENG Xin-Li², CHAI Rui-Juan³

1.College of Urban Construction and Environment, Anhui Science and Technology University, Fengyang 233100, China;2.Landscape development and construction of tianjin co.Ltd, Tianjin 300203, China;3.Department of Bioscience and Engneering, Anhui Technology and Science College, Wuhu 241000, China 1.College of Urban Construction and Environment, Anhui Science and Technology University, Fengyang 233100, China; 2.Landscape development and construction of tianjin co.Ltd, Tianjin 300203, China; 3.Department of Bioscience and Engneering, Anhui Technology and Science College, Wuhu 241000, China

Abstract:

Leaves,stem and root of Impatiens blepharosepala seedling were cultured in vitro,and two types of callus having different differentiation were observed by electron microscope. The results showed that the induction rate was highest on MS+NAA 0.5 mg/L+6-BA 0.5 mg/L,the induction rate of leaves was 100% and the rate of stems was 67%,but the roots can't be induced. Callus of leaves can be induced buds on MS+NAA 0.5 mg/L+6-BA 2.0 mg/L after 40 d,but there was no organ generated from stems. Cells of callus having differentiation arrayed irregularly and there was obviously similar tissue differentiation by electron microscope.

Key words: Impatiens blepharosepala culture in vitro ultrastructural observation