拉雅松遗传多样性及同域分布种间亲缘关系分析

冯源恒<sup>1; 2</sup>; 吴东山<sup>3</sup>; 王德俊<sup>1</sup>; 杨章旗<sup>2</sup>; 李火根<sup>1*</sup>

引用本文:	冯源恒, 吴东山, 王德俊, 杨章旗, 李火根.拉雅松遗传多样性及同域分布种间亲缘关系分析[J].广西植物,2014,(3):320-325.[点击复制]
	FENG Yuan-Heng, WU Dong-Shan, WANG De-Jun, YANG Zhang-Qi, LI Huo-Gen.Genetic diversity of Pinus crassicorticea and its genetic relationship with two sympatric pine species[J].Guihaia,2014,(3):320-325.[点击复制]

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拉雅松遗传多样性及同域分布种间亲缘关系分析
冯源恒^1,2, 吴东山³, 王德俊¹, 杨章旗², 李火根^1*
1. 南京林业大学林木遗传与生物技术省部共建教育部重点实验室, 南京 210037;2. 广西壮族自治区林业科学研究院, 南宁 530002;3. 广西大学, 南宁 530001

摘要:

拉雅松是广西西北部稀有的乡土用材、用脂树种,有较高的经济价值,但其遗传多样性状况及种间进化关系未知。利用SSR分子标记检测拉雅松群体遗传多样性,希望对该物种保护策略的制定提供参考依据。此外,鉴于该地区自然分布的松属树种仅有拉雅松、细叶云南松与马尾松三个种,试图利用SSR分子标记信息分析拉雅松与细叶云南松、马尾松的种间亲缘关系。结果表明:7对SSR引物在拉雅松群体共检测到14个等位基因。有效等位基因数为1.653,观测杂合度为0.577,期望杂合度为0.374,Shannon信息指数为0.540,Nei多样性指数为0.367,表明拉雅松具有较高的遗传多样性。拉雅松与马尾松遗传距离最近为0.0175,与3个细叶云南松群体距离较远,平均为0.0525。拉雅松与马尾松、细叶云南松平均共祖系数(Θ)分别为0.094、0.066,据此推测拉雅松可能与马尾松存在较近的亲缘关系。讨论了拉雅松的遗传多样性保护策略。

关键词: 拉雅松 SSR 遗传多样性同域分布亲缘关系

DOI：10.3969/j.issn.1000-3142.2014.03.008

分类号:Q347; S718.46

基金项目:

Genetic diversity of Pinus crassicorticea and its genetic relationship with two sympatric pine species

FENG Yuan-Heng^1,2, WU Dong-Shan³, WANG De-Jun¹, YANG Zhang-Qi², LI Huo-Gen^1*

1. Key Lab of Forest Genetics and Biotechnology(Nanjing Forestry University), Ministry of Education, Nanjing 210037, China;2. Guangxi Institute of Forestry Science, Nanning 530002, China;3. Guangxi University, Nanning 530001, China 1. Key Lab of Forest Genetics and Biotechnology(Nanjing Forestry University)
, Ministry of Education, Nanjing 210037, China; 2. Guangxi Institute of Forestry Science, Nanning 530002, China; 3. Guangxi University, Nanning 530001, China

Abstract:

Pinus crassicorticea is a rare tree species has high economical value in northwestern Guangxi. As the habitat has been seriously damaged,P. crassicorticea is now regarded as an endangered species. To preserve the germplasm of this species,the genetic diversity of P. crassicorticea was quantified based on 7 SSR loci. The interspecies relationship between P. crassicorticea and two sympatric pine species,P. yunnanensis var. tenuifolia and P. massoniana,was further analyzed. The results showed that the mean number of effective alleles(Ne)per locus was 1.653,the average observed heterozygosity(Ho)was 0.577,the expected heterozygosity(He)was 0.374,Shannon's information index(I)was 0.540 and mean Nei's gene diversity(h)was 0.376,indicating that P. crassicorticea maintains high levels of genetic variation. The genetic identity(GD)was 0.0175 between P. crassicorticea and P. massoniana,while 0.0525 between P. crassicorticea and P. yunnanensis var. tenuifolia. The interspecies relationship coefficient was 0.094 within P. crassicorticea and P. massoniana,and 0.066 in P. crassicorticea and P. yunnanensis var. tenuifolia, respectively. Therefore,it was reasonable that P. crassicorticea was genetically closer to P. massoniana than P. yunnanensis var. tenuifoli. The strategy for the genetic resource conservation of P. crassicorticea was also discussed in this paper.

Key words: Pinus crassicorticea SSR genetic diversity sympatric genetic relationship