两粤黄檀乙醇提取物化学成分分析及抗炎活性研究

梁天坚<sup>2</sup>; 陈 睿<sup>1; 2</sup>; 刘本涛<sup>2</sup>; 袁彩英<sup>2</sup>; 韦建华<sup>2</sup>; 霍丽妮<sup>2</sup>; 李典鹏<sup>1*</sup>

引用本文:	梁天坚, 陈睿, 刘本涛, 袁彩英, 韦建华, 霍丽妮, 李典鹏.两粤黄檀乙醇提取物化学成分分析及抗炎活性研究[J].广西植物,2021,41(7):1035-1045.[点击复制]
	LIANG Tianjian, CHEN Rui, LUI Bentao, YUAN Caiying, WEI Jianhua, HUO Lini, Li Dianpeng.Chemical constituents analysis and anti-inflammatory activities of ethanol extracts from Dalbergia benthamii [J].Guihaia,2021,41(7):1035-1045.[点击复制]

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两粤黄檀乙醇提取物化学成分分析及抗炎活性研究
梁天坚², 陈睿^1,2, 刘本涛², 袁彩英², 韦建华², 霍丽妮², 李典鹏^1*
1. 广西植物功能物质研究与利用重点实验室, 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006;2. 广西中医药大学赛恩斯新医药学院, 南宁 530222

摘要:

为了研究壮药两粤黄檀的化学成分及其抗炎活性,阐明其药理作用机制,该研究采用小鼠二甲苯致炎耳廓肿胀法、角叉菜胶致炎足肿胀急性炎症模型,观察不同剂量两粤黄檀乙醇提取物对小鼠耳廓肿胀度、肿胀率及足肿胀度的影响,并利用液相色谱-质谱(LC-MS)、热裂解气相色谱-质谱(PY-GC/MS)等技术分析提取物的化学成分,结合网络药理学方法对其抗炎活性成分以及作用机制进行初步研究。结果表明:(1)两粤黄檀乙醇提取物具有良好的抗炎活性,高剂量组(8 g·kg^-1)可以显著抑制二甲苯致小鼠耳廓肿胀度、角叉菜胶致炎足趾肿胀,其抑制率均高于50%;(2)从两粤黄檀乙醇提取物中共鉴定得到60个化合物,包括毛蕊异黄酮、芒柄花黄素、血根碱、胡椒碱、β-谷甾醇等多种抗炎活性成分;(3)网络药理学分析显示,两粤黄檀发挥抗炎作用的核心靶点为PIK3CA、EGFR、MAPK、SRC、STAT3、CYP19A1、IL2、MAOA等,涉及cGMP-PKG、cAMP、Focal adhesion、Rap1等信号通路。该研究结果为两粤黄檀的进一步开发利用提供了重要参考。

关键词: 两粤黄檀, LC-MS, PY-GC/MS, 抗炎, 网络药理学

DOI：10.11931/guihaia.gxzw201910006

分类号:Q946

文章编号:1000-3142(2021)07-1035-11

基金项目:国家自然科学基金(21562009); 广西自然科学基金(2017GXNSFAA198171); 广西植物功能物质研究与利用重点实验室开放基金(FPRU2016-9)[Supported by the National Natural Science Foundation of China(21562009); Guangxi Natural Science Foundation(2017GXNSFAA198171); Fund of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization(FPRU2016-9)]。

Chemical constituents analysis and anti-inflammatory activities of ethanol extracts from Dalbergia benthamii

LIANG Tianjian², CHEN Rui^1,2, LUI Bentao², YUAN Caiying², WEI Jianhua², HUO Lini², Li Dianpeng^1*

1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;2. Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine, Nanning 530222, China 1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China; 2. Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine, Nanning 530222, China

Abstract:

Dalbergia benthamii is a traditional Chinese medicine commonly used by Zhuang People in Guangxi, which was reported to possess a variety of biological activities. But their chemical constituents, anti-inflammatory activities and their possible pharmacological mechanism of D. benthamii were unclear yet. The acute inflammation of different doses of ethanol extracts of D. benthamii was observed by the mouse xylene induced ear edema and carrageenan induced paw edema models. Then, the chemical constituents analysis of the extracts were determined by liquid chromatography-mass spectrometry(LC-MS)and pyrolysis gas chromatography-mass spectrometry(PY-GC/MS). Their mechanism of anti-inflammatory activity was analyzed by network pharmacology at the same time. The results were as follows:(1)Compared with the model group, high dose group(8 g·kg^-1)of ethanol extracts of D. benthamii could significantly inhibit the xylene induced mouse ear edema, carrageenan induced hind paw edema, with inhibition rate over 50%.(2)There were sixty compounds identified from the extracts, including some reported anti-inflammatory active constituents, such as isoflavone mullein, formononetin, sanguine, piperine, beta-sitosterol, etc.(3)The network pharmacological analysis were shown that the anti-inflammatory effects of D. benthamii might be related to potential targets of PIK3CA, EGFR, MAPK, SRC, STAT3, CYP19A1, IL2 and MAOA, etc., which were mainly involving signaling pathways of cGMP-PKG, cAMP, Focal adhesion and Rapl. The results will provide an important reference for the further development and utilization of the plants of D. benthamii.

Key words: Dalbergia benthamii, LC-MS, PY-GC/MS, anti-inflammatory, network pharmacological