| 本文已被：浏览 80次 下载 5次 |
|
|
|
| 豌豆EMS突变体库构建及优异突变体筛选 |
|
王东霞1,刘明霞1,姚彦林1,胡静蕾2,魏玉龙3,张怀刚2,刘宝龙2,曹东*2
|
|
(1. 青海大学 农牧学院,西宁 810016；2. 中国科学院西北高原生物研究所/青海省作物分子育种重点实验室,西宁 810008；3. 呼伦贝尔农垦特尼河农牧场有限公司,呼伦贝尔 021500）
|
|
| 摘要: |
| 为创制更丰富的豌豆变异材料，获取优异变异豌豆种质，该研究以‘青建1号’豌豆为试验材料，甲基磺酸乙酯（EMS）作为诱变剂，EMS浓度1%、诱变时间8 h为半致死诱变条件，分析该诱变条件下豌豆植株突变类型，获得突变体重要表型性状数据，建立豌豆表型突变体库，并结合田间表型数据，筛选优异突变体材料。结果表明：（1）通过1%、8 h EMS诱变10 000粒豌豆种子，M1代有4 682株成苗，M2代筛选到342份豌豆突变体。（2）研究发现突变体豌豆表型性状突变类型比较丰富，其中单株籽粒干重变异系数最大，达到0.965。（3）通过对田间表型数据进行综合分析，筛选到10份优异的豌豆突变体种质。该研究结果丰富了豌豆种质资源，可为豌豆相关功能基因挖掘和研究以及优良品种选育提供参考依据。 |
| 关键词: 豌豆，EMS，突变体库，种质资源，优异突变体 |
| DOI：10.11931/guihaia.gxzw202408044 |
| 分类号: |
| 基金项目:青海省科技厅重大专项（2023-NK-A3-B3）；青海省科技成果转化项目（2022-NK-114）；青海省联合专项（LHZX-2021-06）。 |
|
| Construction of EMS mutant library and screening of excellent mutants of pea |
|
WANG Dongxia1, LIU Mingxia1, YAO Yanlin1, HU Jinglei2, WEI Yulong3,ZHANG Huaigang2, LI U Baolong2, CAO Dong2*
|
|
1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810008, China; 2. Northwest Institute of Plateau Biology Chinese Academy of Sciences/Key Laboratory of Crop Molecular Breeding in Qinghai Province, Xining 810008, China; 3. Hulunbuir Nongken Tenihe Agricultural and Pasture Limited Company, Hulunbuir 021500, China
|
| Abstract: |
| To create a broader range of pea mutation materials and acquire superior mutant pea germplasm, ‘Qingjian No. 1’ pea was used as the experimental material and ethyl methane sulfonate (EMS) as the mutagen. The EMS concentration of 1% and mutagenesis time of 8 hours were selected as semi-lethal mutagenesis conditions. The study analyzed the mutation types of pea plants under these conditions and obtained important phenotypic data for the mutants, establishing a pea phenotypic mutant library. Combined with field phenotypic data, excellent mutant materials were selected. The results were as follows: (1) 10 000 pea seeds were treated with 1% EMS for 8 hours, resulting in 4 682 M1 plants and 342 pea mutants in M2 generation. (2) The phenotypic mutation types of mutant peas were diverse, with the coefficient of variation in individual seed dry weight being the largest at 0.965. (3) By comprehensively analyzing the field phenotypic data, 10 excellent pea mutant germplasms were selected. This study enriches the pea germplasm resources and provides a reference for functional gene mining and the breeding of superior pea varieties. |
| Key words: peas, ethyl methane sulfonate (EMS), mutant library, germplasm resource, excellent mutant |
