| 摘要: |
| 细胞分裂素反应调节子（RRs）是细胞分裂素信号转导通路上的重要组分，其中RR22是C型反应调节因子。基于星油藤基因组和转录组数据库，该文克隆了PvoRR22基因，并对其生物信息学以及不同组织部位和6-BA处理花序芽中的表达模式进行了分析。结果表明：（1）PvoRR22编码含170个氨基酸的蛋白。PvoRR22蛋白分子量为18.65 kDa，理论等电点为4.54，且为亲水性蛋白，定位于细胞核。（2）系统进化分析表明星油藤PvoRR22与蓖麻和南欧大戟来源的RR22关系较近。（3）PvoRR22启动子序列含有大量光响应、节律与逆境胁迫响应、以及脱落酸、生长素和茉莉酸等激素响应元件。（4）PlantRegMap分析发现PvoRR22的表达可能受MYB和ERF转录因子家族的调控。（5）PvoRR22主要在星油藤根、茎和茎尖中表达，其中在根中的表达最高；在6-BA处理的花序芽中，PvoRR22的表达量在12 h时达到最高。综上推测，PvoRR22可能在星油藤根、茎和茎尖的生长发育以及细胞分裂素信号转导方面发挥功能。 |
| 关键词: 星油藤, 细胞分裂素反应调节子, PvoRR22, 响应元件, 表达模式 |
| DOI：10.11931/guihaia.gxzw202409010 |
| 分类号: |
| 基金项目:国家自然科学基金（32071739）；中国科学院先导专项（XDA24030502） |
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| Cloning and expression analysis of cytokinin response regulator PvoRR22 in Plukenetia volubilis |
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CHEN Weiyue1,2, FU Qiantang1*
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1. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223; 2. University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Cytokinin response regulators (RRs) are key components of cytokinin signal transduction pathways, and RR22 belongs to type-C RR subfamily. PvoRR22 was cloned based on the Plukenetia volubilis genome and transcriptome database, and its bioinformatics and expression patterns were analyzed in this study. The results were as follows: (1) PvoRR22 encoded a protein of 170 amino acids with a calculated molecular mass of 18.65 kDa and a theoretical isoelectric point of 4.54. PvoRR22 was characterized as a hydrophilic protein and localized in the nucleus. (2) Phylogenetic analysis revealed the closest evolutionary relationship of PvoRR22 was the homologs from Ricinus communis and Euphorbia peplus. (3) Analysis of the PvoRR22 promoter sequences revealed cis-acting elements that responded to light, a circadian rhythm, abiotic stresses, and hormones, including abscisic acid, auxin, and jasmonic acid. (4) PlantRegMap analysis revealed that PvoRR22 might be primarily regulated by the transcription factors of MYB and ERF family. (5) PvoRR22 was principally expressed in the roots, stems, and stem apexes of P. volubilis, with the highest expression levels in the roots. The expression levels of PvoRR22 peaked at 12 h in inflorescence buds after 6-BA treatment. In conclusion, PvoRR22 might play an important role in the growth and development of the roots, stems, and stem apexes in P. volubilis, as well as in cytokinin signal transduction. This study provided valuable information for further research on the function of PvoRR22. |
| Key words: Plukenetia volubilis, cytokinin response regulator, PvoRR22, response elements, expression patterns |
