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| 宽筋藤内生真菌Colletotrichum queenslandicum KJT-1的分离鉴定及其微生物转化研究 |
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张高荣1,杨敏婷1,陈兰燕1,张文秀1,杨浪1,赖红芳1,李俊2*,王爱娟1*
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1. 广西蚕桑丝绸协同创新中心,广西蚕桑生态学与人工智能重点实验室,化学与生物工程学院,河池学院,广西 河池 546300；2.省部共建药用资源化学与药物分子工程国家重点实验室,化学与药学学院,广西师范大学,广西 桂林 541000
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| 摘要: |
| 为充分开发宽筋藤(Tinospora sinensis)的内生真菌资源，获得对宿主药材具有微生物转化能力的菌株，该文采用组织分离法，结合 ITS 序列分析对分离得到的内生真菌进行鉴定；通过抑菌实验、DPPH 自由基清除实验和液质联用技术对微生物转化前后的提取物的生物活性及化学成分差异进行分析评价。结果表明：(1)从宽筋藤中分离得到了1株对宿主药材具有微生物转化能力的内生真菌，并鉴定为 Colletotrichum queenslandicum。(2) 微生物转化前的宽筋藤的各提取物对供试菌株均无抑制作用，而微生物转化后的宽筋藤的正丁醇提取物对金黄色葡萄球菌具有抑制作用，其MBC值为31.3 mg?mL-1。(3) 微生物转化后的宽筋藤的粗提物、正丁醇和乙酸乙酯萃取物清除DPPH 自由基的能力较转化前有所降低，而石油醚萃取物清除DPPH 自由基的能力较转化前有所提高。(4)从微生物转化前后的宽筋藤正丁醇萃取物中分别识别并鉴定出 33和 23 个化合物，转化前后其化学成分发生了改变。该研究从宽筋藤中分离并鉴定了一株对宽筋藤药材具有生物转化能力的内生真菌，为宽筋藤的高效利用及生物转化研究提供了一定的理论支持。 |
| 关键词: 内生真菌，宽筋藤，生物转化，化学成分，生物活性 |
| DOI：10.11931/guihaia.gxzw202408020 |
| 分类号: |
| 基金项目:广西高校中青年教师科研基础能力提升项目(2022KY0611，2024KY0630)；国家自然科学基金(216620040)；广西现代蚕桑丝绸协同创新中心基金资助项目(2023GXCSSC8，2023GXCSSC11)；河池学院科研项目(2020XJYB007)；河池学院高层次人才科研项目(2021GCC018)。 |
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| Isolation, identification and microbial transformation of endophytic fungi Colletotrichum queenslandicum KJT-1 of Tinospora sinensis |
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ZHANG Gaorong1, YANG Minting1, CHEN Lanyan1, ZHANG Wenxiu1, YANG Lang1, LAI Hongfang1, LI Jun2*, WANG Aijuan1*
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1. Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology, School of Chemical and Biological Engineering, Hechi University, Hechi 546300, Guangxi, China; 2. Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
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| Abstract: |
| To fully develop the endophytic fungal resources of Tinospora sinensis and obtain strains with microbial transformation capabilities for the host medicinal material, this study employed a tissue isolation method and ITS?sequence analysis to identify the isolated endophytic fungi and conduct microbial transformation research. The bioactivity and chemical composition differences of the extracts before and after microbial transformation were analyzed and evaluated through antibacterial experiments, DPPH radical scavenging assays, and liquid chromatography-mass spectrometry (LC-MS) techniques. The results were as follows: (1)?An endophytic fungus was isolated and identified as Colletotrichum queenslandicum from Tinospora sinensis, which demonstrated microbial transformation capabilities towards the host medicinal material. (2) The extracts of T. sinensis before microbial transformation had no inhibitory effect on the tested strains. Howeever, after microbial transformation, the n-butanol extract of T. sinensis had inhibitory effect on Staphylococcus aureus, with a minimum bactericidal concentration of 31.3 g·mL-1. (3) The DPPH free radical scavenging ability of crude extract, n-butanol and ethyl acetate extract was decreased compared with that before transformation, while the petroleum ether extract exhibited an enhanced scavenging ability compared with that before transformation. (4) A total of 33 and 23 compounds were identified and characterized from the n-butanol extracts of Tinospora sinensis before and after microbial transformation, respectively, indicating the changes in chemical composition. This study isolated and obtained an endophytic fungus with microbial transformation capabilities of the T. sinensis, providing theoretical support for research on its efficient utilization and biotransformation. |
| Key words: endophytic fungi, Tinospora sinensis, microbial transformation, chemical constituents, biological activity |
