| 摘要: |
| 为了探究厚果崖豆藤化学成分及其对MDA-MB-231细胞的抑制活性，该研究采用硅胶、Sephadex LH-20对厚果崖豆藤提取物进行分离纯化，根据理化特性和波谱数据鉴定化合物的结构，并且采用MTT法测试化合物对MDA-MB-231细胞增值的抑制活性。结果表明：（1）从厚果崖豆藤中分离鉴定出22个化合物，分别鉴定为mtricrin（1）、4-羟基-3-甲氧基苯乙酮（2）、6-甲氧基-7-羟基香豆素（3）、glouxnthone（4）、对甲氧基苯甲酸（5）、oxophoeine（6）、isoshonnin（7）、schizndriside（8）、nudiposide（9）、rtemetin（10）、 hentricontnol（11）、clliphyllin（12）、corymoside（13）、norrchycourmrin（14）、2, 5-二羟基苯乙酮（15）、2, 4-二羟基苯乙酮（16）、mkoline（17）、5, 6, 7-三甲氧基香豆素（18）、5, 7, 4′-三羟基-3′, 5′-二甲氧基黄酮（19）、5, 4’-二羟基-3, 7, 3’-三甲氧基黄酮 （20）、5, 4’-二羟基-7-甲氧基黄酮（21）、3, 5, 7-三羟基-6, 8-二甲基黄酮（22）。所有化合物均为首次从厚崖豆藤中分离鉴定。（2）化合物6和化合物11对MDA-MB-231细胞增殖具有良好的抑制活性，其中化合物6的IC50值为5.71 μmol·L-1，与阳性药物5-氟尿嘧啶相当（IC50值5.53 μmol·L-1）。该研究结果揭示了厚果崖豆藤的化学成分，为深入开展厚果崖豆藤抑制MDA-MB-231细胞增值研究提供科学了依据。 |
| 关键词: 厚果崖豆藤，化学成分，分离鉴定，MDA-MB-231细胞，细胞增值 |
| DOI：10.11931/guihaia.gxzw202410001 |
| 分类号: |
| 基金项目:河南省一流本科课程建设项目（豫教[2021]21548）。 |
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| Chemical constituents of Millettia pachycarpa and their inhibitory activities on MDA-MB-231 cells |
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Zhang Qian1, Miao Yingying1, XU Qiuyue2*
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1. Pingdingshan University, Pingdingshan 467002, Henan, China; 2. The First Affiliated Hospital of Pingdingshan University, Pingdingshan 467006, Henan, China
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| Abstract: |
| In order to investigate the chemical constituents of Millettia pachycarpa and their inhibitory activity on MDA-MB-231 cells, the extract of M. pachycarpa was isolated and purified by silica gel and Sephadex LH-20. The structure of the compound was identified according to the physicochemical properties and spectral data. The inhibitory activity of the compound on MDA-MB-231 cells was tested by MTT assay. The results were as follows show: (1) Twenty-two compounds were identified from the M. pachycarpa as mtricrin (1), 4-hydroxy-3-methoxycetophenone (2), 6-methoxy-7-hydroxycoumrin (3), glouxnthone (4), p-methoxybenzoic acid (5), oxophoeine (6), isoshonnin (7), schizndriside (8), nudiposide (9), rtemetin (10), hentricontnol (11), clliphyllin (12), corymoside (13), norrchycourmrin (14), 2, 5-dihydroxyacetophenone (15), 2, 4-dihydroxyacetophenone (16), mkoline (17), 5, 6, 7-trimethoxycoumarin (18), 5, 7, 4'' -trihydroxy-3'', 5'' -dimethoxyflavonone (19), 5, 4’ 5-dihydroxy-3, 7, 3’-trimethoxyflvnone (20), 5, 4’-dihydroxr-7-methoxyflavone (21), 3, 5, 7-trihydroxy-6, 8-dimethyl flavone (22). All the compounds were identified from the Millettia pachycarpa for the first time. (2) Compounds 6 and 11 showed good inhibitory activity on the proliferation of MDA-MB-231 cells. The IC50 value of compound 6 was 5.71 μmol·L-1, which was similar to that of 5-fluorouracil (IC50 value 5.53 μmol·L-1). The results of this study revealed the chemical composition of Millettia pachycarpa, and provided scientific basis for further research on inhibiting the proliferation of MDA-MB-231 cells. |
| Key words: Millettia pachycarpa, chemical composition, isolation and identification, MDA-MB-231 cells, cell proliferation |
