| 摘要: |
| 丹霞小花苣苔（Primulina danxiaensis）为丹霞地貌特有种，隶属于苦苣苔科，分布狭窄，数量稀少，需要利用植物组织培养技术进行扩繁保育。该文以丹霞小花苣苔叶切片为外植体，通过筛选适宜的HgCl2表面消毒时间﹑不定芽诱导、芽增殖及生根的培养基和组培苗的移栽基质，建立其组培快繁技术体系。结果表明：（1）外植体适宜的消毒条件为75%酒精消毒30 s+0.1% HgCl2消毒6 min，接种存活率达到84.95%。（2）不定芽诱导的培养基为1/2 MS+6-BA 2 mg·L?1+NAA 0.1 mg·L?1，芽诱导率为100%，40 d后平均叶片芽数达到38.35。（3）芽增殖培养基为1/2 MS+6-BA 3 mg·L?1+NAA 0.2 mg·L?1，50 d后芽增殖系数达到7.54。（4）生根培养基为1/2 MS+NAA 0.5 mg·L?1，30 d后生根率为100%，单株生根数达到26.28。（5）组培苗移栽到喀斯特地貌的腐叶土+珍珠岩+蛭石（体积比为1:1:1），泥炭土+珍珠岩+蛭石（体积比为1:1:1），和珍珠岩+蛭石（体积比为1:1）3种基质上成活率达到100%，生长良好，无明显差异。该研究可实现丹霞小花苣苔的大量繁殖，有助于其保护和开发利用。 |
| 关键词: 丹霞小花苣苔，叶片，离体培养，不定芽，植株再生 |
| DOI：10.11931/guihaia.gxzw202409002 |
| 分类号: |
| 基金项目:国家公园建设专项资金（2021GJGY034）；国家中医药管理局项目（GZY-KJS-2018-004）；广东省教育厅项目（2106KZDXM010）；韶关市科技计划项目（220607104530687）；韶关学院“粤北特色南药资源开发利用研究科研团队”项目（韶院 [2023] 74号）；韶关学院农业重点学科项目（韶院 [2023] 73号）。 |
|
| In vitro culture and plant regeneration from the leaves of Primulina danxiaensis |
|
HAN Wei1, CHANG Shengxin1, MAI Guangwei1, ZENG Xiaoyan1, CHEN Zaixiong3, FAN Qiang2*, YU Baiyin1*
|
|
1. Guangdong?Provincial?Key?Laboratory?of?Utilization?and?Conservation?of?Food?and?Medicinal Resources?in?Norther?Region/College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, Guangdong, China; 2. Guangdong Provincial Key Laboratory of Plant Stress Biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; 3. Administrative Commission of Danxiashan National Park, Shaoguan 512300, Guangdong, China
|
| Abstract: |
| Primulina danxiaensis, an endemic species of the Danxia landform within the Gesneriaceae family, exhibits a narrow distribution range and a limited population size, thereby necessitating propagation and conservation via plant tissue culture techniques. In this paper, in order to establish the tissue culture and rapid propagation technical system of P. danxiaensis, the leaf segments of P. danxiaensis were used as explants to screen the appropriate surface disinfection time with HgCl?, the culture media for adventitious bud induction, bud proliferation and rooting, as well as the transplanting substrates for tissue-cultured seedlings. The results were as follows: (1) The optimal disinfection procedure involved a 30-second immersion in 75% alcohol, followed by a 6-minute soak in 0.1% HgCl2, achieving an 84.95% survival rate of leaf explants. (2) For bud induction, the most effective medium was found to be 1/2MS supplemented with 6-benzyladenine (6-BA) 2 mg·L?1 and α-naphthalene aceticacid (NAA) 0.1 mg·L?1, resulting in a 100% bud induction rate and an average of 38.35 buds per leaf explant after 40 days of culture. (3) Bud proliferation was optimally achieved on 1/2MS medium containing 6-BA 3 mg·L?1 and NAA 0.2 mg·L?1 , yielding a proliferation coefficient of 7.54 over a 50-day period. (4) Rooting was successfully induced using 1/2MS medium supplemented with NAA 0.5 mg·L?1 , leading to a 100% rooting rate and an average of 26.28 roots per plant after 30 days. (5) The tissue-cultured seedlings were successfully acclimatized and transplanted into three different mixed substrates: a mixture of leaf mould from Karst landform, perlite, and vermiculite (1:1:1, V/V/V), peat soil, perlite, and vermiculite (1:1:1, V/V/V), and perlite and vermiculite (1:1, V/V), all with a 100% survival rate and demonstrating robust growth. This study is capable of achieving large-scale propagation of P. danxiaensis, a result that significantly contributes to both its resource protection and utilization. |
| Key words: Primulina danxiaensis, leaves, in vitro culture, adventitious bud, plant regeneration |
