| 摘要: |
| 为了解澳洲坚果抗寒性强弱，选育抗寒性强的澳洲坚果品种，该研究采用-8 ℃低温胁迫8个广西澳洲坚果主要栽培品种的枝条，测定叶片超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、可溶性糖（SS）含量、可溶性蛋白（SP）含量、脯氨酸（Pro）含量、丙二醛（MDA）含量、相对电导率（REC）和叶绿素（Chl）含量等抗寒性生理指标，运用相关性分析、主成分分析、隶属函数分析和聚类分析等方法，综合评价澳洲坚果抗寒性强弱。结果表明：（1）低温胁迫下10个抗寒性生理指标差异显著。（2）通过主成分分析可将10个指标转化为3个新指标，累计贡献率达90.194%；结合相关性分析结果，筛选出SOD、POD、SS、SP、Pro、Chl b和Chl（a+b）作为澳洲坚果抗寒性鉴定的主要指标。（3）结合聚类分析结果和综合抗寒能力D值，可将8个澳洲坚果品种按照抗寒能力强弱划分为3类，第1类抗寒性强，包括桂热引4号、桂热7号、HY和JW；第2类抗寒性中等，包括桂热5号和桂热1号；第3类抗寒性弱，包括A16和桂热6号，其中桂热引4号抗寒性最强，桂热6号抗寒性最弱；低温胁迫后的叶片形态观察结果与聚类分析结果相一致。该结论可为澳洲坚果的抗寒性研究提供理论基础，对于澳洲坚果在广西寒区种植及北移种植具有重要意义。 |
| 关键词: 澳洲坚果，低温胁迫，生理指标，抗寒性评价 |
| DOI：10.11931/guihaia.gxzw202408001 |
| 分类号: |
| 基金项目:国家自然科学基金项目（32060652）；广西科技计划项目（桂农科AB241484005）；广西农业科学院基本科研业务专项（桂农科2021YT154，桂农科2023ZX19，桂农科2024YP022） |
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| Comprehensive evaluation of cold resistance of 8 macadamia varieties under low temperature stress |
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HUANG Sijie1, HUANG Yunpeng1, LI Bing1, YE Weiyan1, YANG Xiangyan1, YANG Weihai2*, CAI Yuanbao1
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1. Guangxi Key Laboratory of Quality and Safety Control for Subtropical Fruits, Guangxi Subtropical Crops Research Institute, Nanning 530001, China; 2. College of Life Science and Resources and Environment, Yichun University, Yichun 336000, Jiangxi, China
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| Abstract: |
| In order to select varieties with strong cold resistance, the cold resistance of macadamia varieties were tested. In this study, 8 main cultivated varieties of macadamia in Guangxi were treated at -8 ℃ to determine physiological indicators related to cold resistance, inclulding superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble sugar (SS) content, soluble protein (SP) content, proline (Pro) content, malondialdehyde (MDA) content, relative electric conductivity (REC) and chlorophyll (Chl) content. The cold resistance of macadamia was comprehensively evaluated by correlation analysis, principal component analysis, membership function analysis and cluster analysis. The results were as follows: (1) 10 physiological indicators of cold resistance were significantly different under low temperature stress. (2) By principal component analysis, 10 indicators were converted into 3 new indicators, with a cumulative contribution rate of 90.194%. Combined with the correlation analysis results, superoxide dismutase (SOD), peroxidase (POD), soluble sugar (SS), soluble protein (SP), proline (Pro), chlorophyll b (Chl b) and total chlorophyll (Chl a+b) were selected as the main indicators for the identification of cold resistance of macadamia. (3) Based on the results of cluster analysis and D value of comprehensive cold resistance, 8 macadamia varieties were divided into three categories according to the strength of cold resistance. The first group had the strongest cold resistance, including Guireyin No.4, Guire No.7, HY, and JW. The second group had moderate cold resistance, including Guire No.5 and Guire No.1; The third group had the weakest cold resistance, including A16 and Guire No.6. Among them, Guireyin No.4 had the strongest cold resistance and Guire No.6 had the weakest cold resistance. The observation results of leaf morphology under low temperature stress were consistent with the clustering analysis results. These results provide a theoretical basis for the study of cold resistance of macadamia, which is of great significance for planting macadamia in the cold area of Guangxi and planting in the north of China. |
| Key words: macadamia, low-temperature stress, physiological index, cold resistance evaluation |
