| 引用本文: | 陈超南, 陆嘉惠, 李学禹, 周玲玲, 谢良碧, 李晓岚, 宋 凤.甘草属种间杂交种叶绿体DNA父系遗传的发现及分析[J].广西植物,2017,37(2):162-168.[点击复制] |
| CHEN Chao-Nan, LU Jia-Hui, LI Xue-Yu, ZHOU Ling-Ling,
XIE Liang-Bi, LI Xiao-Lan, SONG Feng.Inheritance analysis and discovery of chloroplast paternal inheritance in interspecific crossing of Glycyrrhiza[J].Guihaia,2017,37(2):162-168.[点击复制] |
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| 甘草属种间杂交种叶绿体DNA父系遗传的发现及分析 |
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陈超南1, 陆嘉惠1,2,3*, 李学禹3, 周玲玲1, 谢良碧1, 李晓岚1, 宋 凤1
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1. 石河子大学 生命科学学院, 新疆 石河子 832003;2. 新疆生产建设兵团绿洲生态农业重点实验室,
新疆 石河子 832003;3. 石河子大学 甘草研究所, 新疆 石河子 832003
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| 摘要: |
| 通过对甘草属乌拉尔甘草(Glycyrrhiza uralensis)、光果甘草(G. glabra)、胀果甘草(G. inflata)及其人工杂交种组合G. uralensis♀ × G. glabra♂、G. glabra♀ × G. uralensis♂、G. uralensis♀ × G. inflata♂、G. inflata♀ × G. uralensis♂共68份材料的核基因ITS序列、叶绿体rbcL、matK、trnH-psbA基因的序列分析,探讨了甘草属叶绿体DNA遗传方式。结果表明:(1)亲本种和人工杂交种ITS序列长度均为614 bp,其中34份人工杂交种ITS序列存在4处变异位点,且人工杂交种均检测出来自父本、母本ITS序列相同位点碱基的叠加,检测率为100%。(2)亲本种与人工杂交种的叶绿体基因rbcL、matK、trnH-psbA序列长度相同,共有4处变异位点,人工杂交种在变异位点处的碱基与其相对应的父本碱基一致率高达97.1%。以上结果说明,该研究获得34份人工杂交种为100%杂交成功的F1子代,核基因ITS序列可用于甘草属杂交种的遗传鉴定; 甘草属叶绿体rbcL、matK、trnH-psbA基因具有父系遗传特性,推测甘草属质体的遗传方式主要表现为父系遗传,这种质体遗传方式的发现为甘草属杂交种和遗传多样性研究提供了新的认识,也为杂交种的亲本鉴定提供分子依据。 |
| 关键词: 甘草属,种间杂交, ITS, 叶绿体DNA, 父系遗传 |
| DOI：10.11931/guihaia.gxzw201512021 |
| 分类号:Q943,Q37 |
| 文章编号:1000-3142(2017)02-0162-08 |
| 基金项目:国家自然科学基金(31260042); 国家重点基础研究发展计划(2014CB954203); 石河子大学高层次人才科研启动项目(RCZX201430)[Supported by the National Natural Science Foundation of China(31260042); National Key Basic Research Program of China(2014CB954203); Startup Program for Scientific Research High-level Personnel of Shihezi University(RCZX201430)]。 |
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| Inheritance analysis and discovery of chloroplast paternal inheritance in interspecific crossing of Glycyrrhiza |
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CHEN Chao-Nan1, LU Jia-Hui1,2,3*, LI Xue-Yu3, ZHOU Ling-Ling1,
XIE Liang-Bi1, LI Xiao-Lan1, SONG Feng1
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1. College of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, China;2. Key Oasis Eco-Agriculture Laboratory of Xinjiang Production
and Construction Group, Shihezi 832003, Xinjiang, China;3. Institute of Licorice in Shihezi University, Shihezi 832003, Xinjiang, China
1. College of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, China; 2. Key Oasis Eco-Agriculture Laboratory of Xinjiang Production
and Construction Group, Shihezi 832003, Xinjiang, China; 3. Institute of Licorice in Shihezi University, Shihezi 832003, Xinjiang, China
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| Abstract: |
| The goal of this paper was to have a better understanding of chloroplast inheritance patterns and identify the hybrid parent in Glycyrrhiza. A total of 68 individuals of Glycyrrhiza were selected including 34 parental species, G. uralensis, G. glabra, G. inflata and 34 artificial interspecific crosses, G. uralensis♀ × G. glabra♂, G. glabra♀ × G. uralensis♂, G. uralensis♀ × G. inflata♂ and G. inflata♀ × G. uralensis♂. Thirty-four artificial interspecific crosses were performed and the sequence polymorphisms of chloroplast DNA(cpDNA)and internal transcribed spacer(ITS)of nuclear gene of parents and progenies were studied. Sequence analysis showed that ITS sequences of parents and progenies had the same length of 614 bp and altogether four variable sites, with three genotypes. Two different ITS sequence copies at four variable sites were found in each individual of F1 hybrids, one from the paternal and the other from the female parent, about which the detection ratio was 100%. It suggested that 34 artificial interspecific crosses produced successful F1 hybrids in this research and genetic trait of ITS could infer parents of Glycyrrhiza. Sequence analysis showed that the cpDNA rbcL, matK and trnH-psbA sequences of parents and artificial interspecific crosses had the same length, within a total of four variable sites and six genotypes. Two different cpDNA haplotypes were found in Glycyrrhiza. The cpDNA haplotypes of F1 hybrids matched those of the male parents and that ratio was up to 97.1%, indicating that the genetic trait was mainly inherited from the paternal parent. This result demonstrated that the chloroplast inheritance pattern of Glycyrrhiza was mostly paternal inheritance, which provides new insights into the use of cpDNA markers in studies of natural hybrids and genetic polymorphism in Glycyrrhiza. |
| Key words: Glycyrrhiza, interspecific crossing, ITS, chloroplast DNA, paternal inheritance |
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