| 引用本文: | 曾 琳, 吴 怡, 何明军, 顾雅坤, 王德立.超低温冷冻对益智种子生理生化特性的影响[J].广西植物,2018,38(4):529-535.[点击复制] |
| ZENG Lin, WU Yi, HE Mingjun, GU Yakun, WANG Deli.Physiological and biochemical characteristics of Alpinia oxyphylla seeds after cryopreservation[J].Guihaia,2018,38(4):529-535.[点击复制] |
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| 超低温冷冻对益智种子生理生化特性的影响 |
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曾 琳, 吴 怡, 何明军, 顾雅坤, 王德立*
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中国医学科学院药用植物研究所 海南分所, 海口 570311
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| 摘要: |
| 益智( Alpinia oxyphylla)是我国四大南药之一,其种子为顽拗性种子,不耐干燥和低温,无法常规保存。为解决益智种子长期稳定保存的问题,该研究以益智种子为材料,从种子含水量以及冷冻方式方面优化益智种子超低温保存方法,并对比液氮冷冻前后种子发芽率、α-淀粉酶活性、超氧化物歧化酶活性、过氧化物酶活性、脱氢酶活性、丙二醛含量等活力指标,分析液氮超低温冷冻对益智种子生理生化特性的影响。结果表明:直接液氮冷冻含水量13.92%～14.38%范围的种子是益智种子超低温保存的最佳条件。液氮冷冻后,随着超低温冷冻时间的延长,益智种子发芽率先由76.76%下降至52.5%,再上升至65%,最后下降并稳定在50%以上。其种子α-淀粉酶活性先下降后上升,然后又下降,最后上升并稳定至冷冻前的水平。丙二醛含量则是先上升后下降,最后趋于平稳状态。过氧化物酶活性是先下降,然后稳定在恒定水平。超氧化物歧化酶活性是随着冷冻时间的延长而持续上升,脱氢酶活性则是持续下降。以上结果说明,液氮冷冻时间对益智活力有一定的影响,但对种子有些性能具有一定的促进作用。无论是发芽率,还是生理生化指标,都不同程度表明液氮超低温保存益智种子是可行的。 |
| 关键词: 超低温保存, 益智种子, 含水量, 生理生化特性, 种子发芽率 |
| DOI：10.11931/guihaia.gxzw201704031 |
| 分类号:Q945 |
| 文章编号:1000-3142(2018)04-0529-07 |
| 基金项目:海南省中药现代化项目(2015ZY03); 中央高校基本科研业务费专项(协和青年基金)(3332016071); 海南省重点研发计划项目(ZDYF2016145)[Supported by the Program of Chinese Medicine Modernization in Hainan(2015ZY03); Fundamental Research Fund for the Central Universities(PUMC Youth Fund)(3332016071); Key R & D Program in Hainan(ZDYF2016145)]。 |
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| Physiological and biochemical characteristics of Alpinia oxyphylla seeds after cryopreservation |
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ZENG Lin, WU Yi, HE Mingjun, GU Yakun, WANG Deli*
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1.Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical
Sciences &2.Peking Union Medical College, Haikou 570311, China
Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical
Sciences & Peking Union Medical College, Haikou 570311, China
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| Abstract: |
| Alpinia oxyphylla was one of the four famous south medicines in China. Its seeds were the recalcitrant seeds, which could not be preserved in dried and frozen environment, therefore, the seeds of Alpinia oxyphylla could not be reserved by regular way, in low temperature and low humidity conservation. In order to solve the A. oxyphylla seeds with long-term and stability storage, we focused on seed moisture content and freezing manner, cryopreservation method optimization, seed vigor indexes under liquid nitrogen, and the effects of cryopreservation on the physiological and biochemical characteristics. The seed vigor indexes, including germination, activity of α-amylase, SOD, POD, dehydrogenase, MDA. The results showed that the A. oxyphylla seeds with water content of 13.92%-14.38% cryopreservated by rapid freezing could obtain the optimum effect. After liquid nitrogen freezing, the germination rate of the seeds decreased significantly, but with the extension of the freezing time, the germination rate was more than 50%. There was no significant effect on the activity of α-amylase and the content of MDA in liquid nitrogen, a decrease in the dehydrogenase and POD activity of seeds after freezing, and the activity of SOD increased with the adding of freezing time. Above results indicate that freezing time have a certain influence on seeds vigor of A. oxyphylla, but it can promote some performance of seeds. Both in germination rate, and physiological and biochemical indexes, show that A. oxyphylla seeds preserved with liquid nitrogen is feasible. |
| Key words: cryopreservation, seeds of Alpinia oxyphylla, water content, physiological and biochemical characteristics, seed germination rate |
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