八角莲组织培养研究

唐凤鸾; $2; 黄宁珍; 付传明; 赵志国

引用本文:	唐凤鸾李锋黄宁珍付传明赵志国.八角莲组织培养研究[J].广西植物,2008,(6):819-822.[点击复制]
	TANG Feng-Luan, LI Feng, HUANG Ning-Zhen, FU Chuan-Ming, ZHAO Zhi-Guo.Study on tissue culture of Dysosma versipellis[J].Guihaia,2008,(6):819-822.[点击复制]

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八角莲组织培养研究
唐凤鸾李锋黄宁珍付传明赵志国
广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006

摘要:

以八角莲种子为外植体,MS为基本培养基,通过不同的激素种类和浓度配比,对八角莲进行组织培养研究。结果表明:种子在MS+BA1.0 mg/L+IBA0.5 mg/L+GA₃4.0 mg/L培养基上容易萌芽,发芽率为72.4%; 培养基MS+BA10.0 mg/L+GA₃0.5 mg/L可诱导种子幼苗形成丛生芽; 继代繁殖在MS+BA(8.0～10.0)mg/L+GA₃2.0 mg/L与低浓度BA或无BA的培养基上进行循环培养效果较好; MS+NAA1.0 mg/L+AC0.2 g/L适宜诱导生根获得再生植株,生根率100%。带叶叶柄在MS+BA1.0 mg/L+2-ip(0.5～1.0)mg/L+NAA0.02 mg/L培养基上可诱导愈伤及根,直接形成再生植株。生根苗移栽成活率90%。

关键词: 八角莲组织培养激素

DOI：

分类号:Q943.1

基金项目:

Study on tissue culture of Dysosma versipellis

TANG Feng-Luan, LI Feng, HUANG Ning-Zhen, FU Chuan-Ming, ZHAO Zhi-Guo

Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China

Abstract:

The seeds of Dysosma versipellis were used as explants for tissue culture in different culture media. The results obtained from the present study were as follows:the seed germination rate could reach 72.4% on MS+BA1.0 mg/L+IBA0.5 mg/L+GA₃4.0 mg/L; the medium which was good for the cluster buds induction in seed buds was MS+BA10.0 mg/L+GA₃0.5 mg/L; the medium of proliferation should be MS+BA(8.0～10.0)mg/L+GA₃2.0 mg/L with low concentration of BA or no BA for circulation culture; the ideal medium for root formation was MS+NAA1.0 mg/L+AC0.2 mg/L,and the rooting rate could reach 100%; tender leaves with stalk could be induced for callus and roots in MS+BA1.0 mg/L+2-ip(0.5～1.0)mg/L+NAA0.02 mg/L. The survival rate of the seedlings was about 90%.

Key words: Dysosma versipellis tissue culture hormone