| 引用本文: | 丁 兰 李 娟 杨 红 祁林林.离体条件下卡德丽亚兰的形态建成[J].广西植物,2009,(3):382-385.[点击复制] |
| DING Lan, LI Juan, YANG Hong, QI Lin-Lin.Morphogenesis of the protocorm of Cattleya hybird in vitro[J].Guihaia,2009,(3):382-385.[点击复制] |
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| 摘要: |
| 采用光学显微镜和电子显微镜技术对离体培养条件下卡德丽亚兰原球茎产生及形态建成进行了系统的细胞学观察。结果表明,离体培养条件下卡德丽亚兰原球茎起源于母原球茎表皮、亚表皮或中央薄壁细胞,这些细胞形成胚性细胞后,通过不断的细胞分裂形成胚性细胞团,胚性细胞团保持细胞分裂的同时伴随着胚性细胞的液泡化过程,形成了由上百个薄壁细胞组成的原球茎; 原球茎通过芽端分生组织区形成,进一步分化成芽,其下方有输导组织形成。这一过程几乎重演了其合子胚发育及分化的过程。原球茎生长过程中其薄壁细胞通过叶绿体储存大量淀粉粒,而在以后的形态建成中淀粉粒逐渐降解。 |
| 关键词: 卡德丽亚兰 形态建成 原球茎 离体培养 |
| DOI： |
| 分类号:Q944-33 |
| 基金项目: |
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| Morphogenesis of the protocorm of Cattleya hybird in vitro |
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DING Lan, LI Juan, YANG Hong, QI Lin-Lin
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College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
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| Abstract: |
| Formation and morphogenesis of the protocorms of Cattleya hybrid were investigated in cytology by light microscopy and electronic microscopy. The results indicated that the protocorms consist of numerous parenchymatous cells coming from proliferation of few embryonic cells originated from epidermal cell or subepidermal cell or parenchymatous cell of mother protocorms. While protocorm was cultured on differentiation medium, the meristematic dome was formed at its anterior end,which developed into a bud subsequently. The differentiation of the conducting tissue always occurred at the opposite end. The developmental mode of the protocorms in vitro is very similar to that of its zygotic embryo. A lot of starch grains were accumulated in the chloroplasts of parenchymatous cells when the protocorms grew up and the starch grains were decomposed during the process of morphogenesis. |
| Key words: Cattleya morphogenesis protocorm culture in vitro |
