| 引用本文: | 黄 华, 赖华清, 蒋 勇, 李仁清, 李风琴, 刘 勇, 徐艳琴.箭叶淫羊藿与近缘种天平山淫羊藿花粉形态对比研究[J].广西植物,2020,40(9):1300-1314.[点击复制] |
| HUANG Hua, LAI Huaqing, JIANG Yong, LI Renqing, LI Fengqin, LIU Yong, XU Yanqin.Comparative study on pollen morphology of Epimedium sagittatum and its sibling species, E. myrianthum[J].Guihaia,2020,40(9):1300-1314.[点击复制] |
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| 箭叶淫羊藿与近缘种天平山淫羊藿花粉形态对比研究 |
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黄 华, 赖华清, 蒋 勇, 李仁清, 李风琴, 刘 勇, 徐艳琴*
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江西中医药大学 药学院, 南昌 330004
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| 摘要: |
| 该研究采用扫描电子显微镜(SEM, scanning electron microscope)对箭叶淫羊藿13个居群(含箭叶淫羊藿光叶变种1个居群)、天平山淫羊藿7个居群进行花粉形态和外壁纹饰的观察及对比研究。结果表明:两个类群的花粉均为近球形,具三孔沟,大小相近。箭叶淫羊藿的花粉大小为(18.21～21.66)μm ×(19.55～23.63)μm, 天平山淫羊藿的花粉大小为(18.32～21.43)μm ×(19.73～23.48)μm。外壁纹饰是花粉形态的重要性状。不同居群间花粉外壁纹饰存在较大差异,主要分为网纹纹饰、条网纹纹饰和颗粒状纹饰三大类,继而根据网脊类型和网眼密度分为六亚类。其中,箭叶淫羊藿的花粉形态包含了全部的三大类六亚类,天平山淫羊藿包含其中的三大类四亚类。天平山淫羊藿花粉特征为箭叶淫羊藿花粉形态变异的子集。整体而言,外壁纹饰类型随纬度和海拔增加呈现出由网状纹饰向条网状纹饰过渡的趋势。花粉主要形态性状特征均与海拔存在密切相关性。在花粉形态及外壁纹饰特征上,天平山淫羊藿在箭叶淫羊藿变异范围之内,与其他宏观形态性状变异一致。花粉形态性状无法将两类群有效区分,未能提供天平山淫羊藿作为独立种的有力支撑。这暗示花粉形态证据在厘清近缘类群之间的系统发育关系意义相对有限,但能否对该属系统发育树大支的界定提供有力证据还有待于进一步研究。 |
| 关键词: 居群, 箭叶淫羊藿, 天平山淫羊藿, 花粉形态, 形态变异 |
| DOI：10.11931/guihaia.gxzw201912012 |
| 分类号:Q944 |
| 文章编号:1000-3142(2020)09-1300-15 |
| 基金项目:国家自然科学基金(31360036); 江西省自然科学基金(20181BAB205075); 江西省青年科学家(井冈之星)培养对象计划项目(20133BCB23024); 江西中医药大学中药学一流学科科研启动项目(JXSYLXK-ZHYAO023)[Supported by the National Natural Science Foundation of China(31360036); the National Science Foundation of Jiangxi Province(20181BAB205075); Yong Scientists Fellowship of Jiangxi Province(20133BCB23024); the Scientific Research Foundation for the First-class Discipline of Chinese Medicine Program of Jiangxi University of Traditional Chinese Medicine(JXSYLXK-ZHYAO023)]。 |
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| Comparative study on pollen morphology of Epimedium sagittatum and its sibling species, E. myrianthum |
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HUANG Hua, LAI Huaqing, JIANG Yong, LI Renqing, LI Fengqin, LIU Yong, XU Yanqin*
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College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
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| Abstract: |
| Epimedium myrianthum having much in common with E. sagittatum. There are transitions in many morphological characters and even in key taxonomy features. In order to explore the taxonomic relationship between the two taxa, the pollen morphology and ornamentation of exine of 13 populations of E. sagittatum(including 1 population of E. sagittatum var. glabratum T. S. Ying)and 7 populations of E. myrianthum were observed and compared by scanning electron microscope(SEM). The results showed that the pollen of both taxa was subspheroidal, with three-hole groove. Pollen size of E. sagittatum and E. myrianthum was(18.21-21.66)μm ×(19.55-23.63)μm and(18.32-21.43)μm ×(19.73-23.48)μm, respectively. The ornamentation of exine was an important character of pollen morphology. There were significant differences in ornamentation of exine among populations, which have been divided into three categories, namely reticulate, article reticulate and granular, and then divided into six subcategories according to the type of mesh ridge and mesh density. Among them, pollen morphology of E. sagittatum(including E. sagittatum var. glabratum)contained all three categories and six subcategories, and that of E. myrianthum contained three categories and four subcategories. This means that the pollen morphological variation pattern of E. myrianthum was a subset of E. sagittatum. On the whole, the variation pattern of the ornamentation of exine showed a trend, from reticulate to article reticulate, with the increase of latitude. And the main morphological characters of pollen were closely related to the elevation. As far as the pollen morphology concerned, the variation pattern of E. myrianthum was within the variation range of E. sagittatum, which was consistent with the variation patterns of other morphological characters. Pollen morphological characters could not distinguish the two taxa effectively, which failed to provide strong support for E. myrianthum as an independent species. It is suggested that the pollen morphological evidence is of relatively limited in clarifying the phylogenetic relationship among related taxa. However, further studies are still needed to provide strong evidence for the definition of large branches of phylogenetic trees of this genus. |
| Key words: population, E. sagittatum, E. myrianthum, pollen morphology, morphological variation |
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