| 摘要: |
| 建立了胶束电动毛细管电泳色谱法(MECC)测定红丝线提取物中紫蓝素的含量的方法。毛细管柱内径75 μm，长50.2 cm；运行电压25 kV，检测波长585 nm，温度25 ℃；缓冲液为25 mmol/L，β CD 10 mmol/L，硼酸盐 20%乙腈（pH值8.0）；进样方式：压力进样，进样时间5 s。结果表明紫蓝素在10～100 μmol/L浓度范围内具有良好的线性关系(r=0.9995)，回收率为95.3%～103.2%。该方法快速、简便、并且较为准确，适用于测定红丝线提取物中紫蓝素的含量。 |
| 关键词: 胶束电动毛细管电泳色谱法 紫蓝素 红丝线提取物 |
| DOI： |
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| Determination of zilanine contents in extracts of Peristrophe baphica by Micellar Eletrokinetic Capillary Chromatography (MECC) |
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LI Juan， XIE YunChang*， JIANG XiaoHua
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Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences， Guilin 541006， China
Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences， Guilin 541006， China
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| Abstract: |
| The method of MECC was established to determine zilanine contents in extracts of P.baphica. The capillary column was 50.2 cm long and 75 μm in internal diameter；the operation voltage was 25 kV，testwavelength 585 nm，column temperature 25 ℃；the mobile buffer was 25 mmol/L boric acid 20% acetonitrile(pH 8.0)with 10 mmol/L β CD；the sampling was conducted by pressure for 5 s. The results showed that the linear range of determination for zilanine was 10～100 μmol/L(r=0.9995). The average recovery rate was 953%-103.2%. The method was fast，simple，convenient，comparatively accurate，and could be used to determinate zilanine contents in extract of P.baphica. |
| Key words: MECC Zilanine extract of Peristrophe baphica |
