| 引用本文: | 李 歌, 凌少军, 陈伟芳, 任明迅, 唐 亮.昌化江河谷隔离对海南岛特有植物盾叶苣苔遗传多样性的影响[J].广西植物,2020,40(10):1505-1513.[点击复制] |
| LI Ge, LING Shaojun, CHEN Weifang, REN Mingxun, TANG Liang.Effects of geographic isolation caused by Changhua River on genetic diversity of Hainan-endemic Metapetrocosmea peltata(Gesneriaceae)[J].Guihaia,2020,40(10):1505-1513.[点击复制] |
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| 昌化江河谷隔离对海南岛特有植物盾叶苣苔遗传多样性的影响 |
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李 歌1, 凌少军1, 陈伟芳1, 任明迅1, 唐 亮1,2*
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1. 海南大学 热带农林学院, 环南海陆域生物多样性研究中心, 海口 570228;2. 海南大学 热带雨林国家公园研究院, 海口 570228
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| 摘要: |
| 为评估盾叶苣苔的遗传多样性与遗传分化格局,探索影响盾叶苣苔遗传变异地理分布的因素,该研究采集盾叶苣苔(Metapetrocosmea peltata)11个种群172份材料,通过PCR扩增和测序分析核糖体转录间隔区(ITS)序列的变异式样。结果表明:(1)盾叶苣苔物种水平的遗传多样性很高(HT=0.998, π=0.023 5),种群间基因流很弱(Nm=0.04)且存在强烈的遗传分化(GST=0.375)。(2)单倍型分析显示,盾叶苣苔的单倍型大多是种群特异的,仅白马岭与南茂岭种群有共享单倍型。(3)Mantel test表明,遗传距离和地理距离存在一定相关性(相关系数r=0.322,P=0.010)。(4)Structure聚类分析将盾叶苣苔划分为6种遗传成分,其地理分布与昌化江河谷导致的隔离样式基本一致,基于Nei遗传距离的种群聚类分析支持这一结果,显示盾叶苣苔遗传多样性的分布受到昌化江河谷的隔离作用。(5)AMOVA分析确定67%的变异来自地区间,表明地理隔离是盾叶苣苔种群分化的重要因素。这表明昌化江及其支流所引起的海南岛山地内部隔离是盾叶苣苔种群发生强烈遗传分化的重要原因,从而导致盾叶苣苔在物种水平具有较高的遗传多样性。上述研究结果为海南特有苦苣苔资源盾叶苣苔的保护和可持续利用提供了理论指导,将有助于理解海南岛特有植物和其他海岛植物遗传变异的地理分布及其影响因素。 |
| 关键词: 盾叶苣苔, 遗传多样性, 遗传结构, 地理隔离, 岛屿特有种 |
| DOI：10.11931/guihaia.gxzw201808024 |
| 分类号:Q948.15 |
| 文章编号:1000-3142(2020)10-1505-09 |
| 基金项目:国家自然科学基金(41661010, 31670230); 海南省自然科学基金创新研究团队项目(2018CXTD331); 海南大学科研启动项目(kyqd1613)[Support by the National Natural Science Foundation of China(41661010,31670230); Hainan Provincial Innovative Reasearch Team Program(2018CXTD331); Research Start-up Fund of Hainan University(kyqd1613)]。 |
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| Effects of geographic isolation caused by Changhua River on genetic diversity of Hainan-endemic Metapetrocosmea peltata(Gesneriaceae) |
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LI Ge1, LING Shaojun1, CHEN Weifang1, REN Mingxun1, TANG Liang1,2*
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1. Research Center for Terrestrial Biodiversity of South China Sea, Institute of Tropical Agriculture and Forestry, Hainan University,
Haikou 570228, China;2. Institute of Tropical Rain Forest National Park, Hainan University, Haikou 570228, China
1. Research Center for Terrestrial Biodiversity of South China Sea, Institute of Tropical Agriculture and Forestry, Hainan University,
Haikou 570228, China; 2. Institute of Tropical Rain Forest National Park, Hainan University, Haikou 570228, China
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| Abstract: |
| Metapetrocosmea peltata(Gesneriaceae)is an endemic species distributed in the middle and southern mountainous area of Hainan Island. In this study, we collected 172 M. peltata individuals from 11 natural populations, and assessed the genetic diversity and differentiation of this species based on sequence variation of intergenic transcribed spacer(ITS)region. In addition, the factors that may impact the level and distribution of genetic diversity were explored. The results were as follows:(1)M. peltata had a high level of genetic diversity at its species level(HT=0.998, π=0.023 5), however, the gene flow between populations was very weak(Nm=0.04), whereas the genetic differentiation was quite strong(GST=0.375).(2)Most haplotypes of M. peltata were population specific, only except for BM and NM populations, which shared a few haplotypes.(3)Mantel test showed that the genetic distances between haplotypes were correlated with their geographic distances(correlation index r=0.322, P=0.010).(4)Structure analysis clustered individuals of M. peltata into six genetic groups, whose geographic distributions were largely concordant with the isolation pattern of Hainan mountainous area caused by Changhua River. The geographic distribution of genetic clusters was further supported by population cluster analysis based on Nei's genetic distance. This study manifests the isolation effect of Changhua River on the distribution of genetic diversity of M. peltata.(5)AMOVA analysis showed that 67% genetic variation was partitioned among populations, suggesting genetic differentiation of this species was mainly caused by geographic isolation. In conclusion, geographic isolation of Hainan mountainous area caused by Changhua River and its tributaries should be the driving force for the strong genetic differentiation among M. peltata populations, and also may lead to the high genetic diversity detected at the species level of M. peltata. This study not only provides recommendation for efficient conservation and utilization of M. peltata, a potential ornamental plant species, but also facilitates the understanding of geographic distribution of genetic variation for Hainan Island endemic and other plant species. |
| Key words: Metapetrocosmea peltata, genetic diversity, genetic constructure, geographic isolation, island endemic species |
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