摘要: |
用聚丙烯酰胺凝胶电泳技术对淫羊藿属植物10个种(含7个大花种和3个小花种)32个幼叶样品的谷草转氨酶同工酶进行了研究。结果发现,谷草转氨酶同工酶酶带共15条。不同物种的酶带数为2~15条,不同植株的谱带数为2~10条。种间存在明显差异,可以作为物种鉴定的辅助依据。 |
关键词: 淫羊藿属 谷草转氨酶同工酶 系统关系 |
DOI:10.3969/j.issn.1000-3142.2011.01.022 |
分类号:Q945 |
基金项目: |
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Glutamate oxaloacetate transaminase isozyme in 10 species of genus Epimedium |
NING Li-Li, CHEN Qing-Fu*
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Institute of Plant Genetics and Breeding, School of Life Science, Guizhou Normal University, Guiyang 550001, China
Institute of Plant Genetics and Breeding, School of Life Science, Guizhou Normal University, Guiyang 550001, China
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Abstract: |
The glutamate oxaloacetate transaminase isozyme in 32 young leaf samples of 10 species of Epimedium(including 7 species of the big-flower group and 3 species of the small-flower group)were studied by means of polyacrylamide gel eletrophoresis(PAGE). The results showed that 15 bands of glutamate oxaloacetate transaminase isozyme were discovered in all accessions,likewise,2-15 bands in different species and 2-10 in different plant individuals,showing that zymographs among species were obviously different. Therefore,this could be an auxiliary evidence for the species identification. |
Key words: Epimedium glutamate oxaloacetate transaminase isozyme phylogeny |