摘要: |
采用气相色谱 质谱联用技术对小叶红叶藤挥发油的化学成分进行分析,并用DPPH•法、ABTS•+法和铁氰化钾还原法对其抗氧化能力进行研究。结果表明:从小叶红叶藤挥发油分离出221个色谱峰,共鉴定64个化合物,占挥发油总量的9572%,主要成分为dl 薄荷酮(5343%)、(E) 薄荷酮(1420%)、5 甲基 2 异丙基 3 环己烯 1 酮(987%)、β 萜品烯(816%)、β 蒎烯(154%)、α 蒎烯(118%)等。抗氧化实验中,不同浓度的挥发油对DPPH和ABTS+自由基有较好的清除能力,最高清除率分别达到6225%和6811%,但其还原能力较弱,抗氧化能力均具有明显的量 效关系。 |
关键词: 小叶红叶藤 挥发油 抗氧化性 气相色谱 质谱联用 |
DOI: |
分类号:R285.5 |
基金项目: |
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Chemical constituents and antioxidant activity of essential oils from Rourea microphylla in Guangxi |
HUO LiNi1*, LI PeiYuan1, CHEN Rui2, DENG ChaoCheng1, LU RuMei1, TENG MingMin1
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1.Guangxi Traditional Chinese Medical University, Nanning 530001, China;2.2.Guangxi Grain and Oil Scientific Institute, Nanning 530001, China
1.Guangxi Traditional Chinese Medical University, Nanning 530001, China; 2.Guangxi Grain and Oil Scientific Institute, Nanning 530001, China
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Abstract: |
The constituents of the essential oils of Rourea microphylla from Guangxi were separated and identified by GC MS. The scavenging activities on DPPH• radical,ABTS•+radical and reducing power were detected by UV Vis spectrophotometry. 221 compounds were isolated and 64 compounds were identified that composed about 9572% of the total essential oils. The principal chemical constituents of the essential oils were dl Menthone(5343%),(E) Menthone(1420%),2 isopropyl 5 methyl 3 Cyclohexen 1 one(987%),β Terpinene(816%),β Pinene(154%),α Pinene(118%),et al. These oils possessed good scavenging activities on both DPPH• and ABTS•+ radical,the scavenging rate reaching to 6225% and 6811%,respectively,but showed low activities on reducing power. All antioxidant activity showed a concentration effect relationship. |
Key words: Rourea microphylla essential oils antioxidant activity GC MS. |