| 引用本文: | 刘世彪, 谭秀梅, 彭小列, 杨 鹏, 吕江明.绞股蓝种子油的提取、成分分析和急性毒性实验[J].广西植物,2014,(1):130-134.[点击复制] |
| LIU Shi-Biao, TAN Xiu-Mei,PENG Xiao-Lie, YANG Peng, LÜ Jiang-Ming.Extraction, composition analysis and acute toxicity test of seed oil of Gynostemma pentaphyllum[J].Guihaia,2014,(1):130-134.[点击复制] |
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| 绞股蓝种子油的提取、成分分析和急性毒性实验 |
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刘世彪, 谭秀梅, 彭小列, 杨 鹏, 吕江明
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1. 吉首大学 植物资源保护与利用湖南省高校重点实验室, 湖南 吉首 416000;2. 吉首大学 医学院, 湖南 吉首 416000
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| 摘要: |
| 以石油醚为提取剂、料液比为1:7,进行单因子和正交试验及急性毒性实验。结果表明:超声波辅助提取绞股蓝种子油的最适工艺条件为提取的温度60 ℃、功率200 W和时间50 min,此条件下种子油提取率为28.13%; 绞股蓝种子油的密度为0.895 g·mL-1、折光值1.518 nD20、酸值0.85和皂化值180.2 mg KOH·g-1、碘值135.7 g I2·100g-1,为优良的干性油; 绞股蓝种子油含有10种脂肪酸成分,主要为亚麻酸(80.49%)、油酸(8.05%)、亚油酸(7.08%)、硬脂酸(1.79%)和棕榈酸(1.28%),不饱和脂肪酸质量分数达总脂肪酸的96.48%; 以剂量 65 mL·(kg·d)-1的绞股蓝种子油灌胃小鼠,在连续14 d的实验期内,没有发现小鼠的中毒症状和死亡现象。初步判定绞股蓝种子油无急性毒性,有望作为营养保健油源进一步开发。 |
| 关键词: 绞股蓝 种子油 理化性质 脂肪酸组成 急性毒性实验 |
| DOI：10.3969/j.issn.1000-3142.2014.01.025 |
| 分类号:Q646.4 |
| 基金项目: |
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| Extraction, composition analysis and acute toxicity test of seed oil of Gynostemma pentaphyllum |
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LIU Shi-Biao1*, TAN Xiu-Mei1,PENG Xiao-Lie1, YANG Peng1, LÜ Jiang-Ming2
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1. Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province,Jishou University,
Jishou 416000, China;2. College of Medical,Jishou University, Jishou 416000, China
1. Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province,Jishou University,
Jishou 416000, China; 2. College of Medical,Jishou University, Jishou 416000, China
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| Abstract: |
| Based on petroleum ether as extraction solvent and ratio of material to solution 1:7,the ultrasonic-assisted extraction process on seed oil of Gynostemma pentaphyllum was optimized by single factor test and orthogonal test. The optimal ultrasonic-assisted conditions were as follows:extraction temperature 60 ℃,ultrasonic power 200 W and extraction time 50 min,under which the extraction rate of seed oil could reach 28.13%. Physicochemical properties of the seed oil including density,dioptre,acid value,saponification value and iodine value were respectively 0.895 g·mL-1,1.518 nD20,0.85,180.2 mg KOH·g-1 and 135.7 g I2·100g-1,which revealed that G. pentaphyllum seed oil was a fine drying oil. GC-MS analysis revealed that there were 10 kinds of fatty acids in G. pentaphyllum seed oil,and the mass fraction were linolenic acid(80.49%),oleic acid(8.05%),linoleic acid(7.08%),stearic acid(1.79%)and palmitic acid(1.28%). The unsaturated fatty acids accounted for 96.48% of total fatty acids. In the acute toxicity test,a dose of 65 mL·(kg · d)-1 of seed oil were filled into mice's stomach,and no evident poisoning symptoms and animal death were detected after 14 d continuous observation,which preliminarily indicated that G. pentaphyllum seed oil was of nontoxicity,and expected to be developed as a resource of health oil. |
| Key words: Gynostemma pentaphyllum seed oil physicochemical property fatty acid composition acute toxicity |
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