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| 二穗短柄草BdFKF1基因调控烟草开花的转录组学分析 |
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杨龙姣1, 李亚娇2, 陈锡2,马培杰2, 罗文举1, 陈才俊2, 陈莹2,刘晓霞2,王小利2*
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(1. 贵州大学 动物科学学院草业科学系,贵阳 550025；2. 贵州省农业科学院草业研究所,贵阳 550006）
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| 摘要: |
| FKF1是响应蓝光节律表达的基因，是光周期途径调控植物开花的重要因子之一。为探索BdFKF1基因在光周期途径中调控烟草植株开花的分子机制，该研究以野生型烟草（SR1）和转BdFKF1基因（BdFKF1-OE）烟草植株为材料，利用转录组学测序并进行RT-qPCR验证，观测并记录两种材料的开花时间。结果表明：（1）在SR1 vs FKF1组中，共筛选出472个差异表达基因、上调基因248个、下调基因224个，其中与光周期相关的差异表达基因有14个、上调基因7个、下调基因7个。（2）GO富集分析发现，差异表达基因大量富集在U5 小核核糖核蛋白和氧化还原酶活性通路中，作用于成对供体、蓝光反应等通路中，其中与光周期相关的差异表达基因主要富集在FK506结合、光周期，开花、光周期、磷脂酰乙醇胺结合、大环内酯结合、花发育调控等通路中。（3）KEGG富集分析发现，在ABC转运蛋白、内质网中的蛋白质加工、角质，木栓素和蜡的生物合成、昼夜节律-植物等通路中，其中与光周期相关的差异表达基因主要富集在植物激素信号转导、昼夜节律?植物通路中。（4）通过观察记录发现BdFKF1-OE植株开花时间比SR1提前3.9 d。（5）RT-qPCR结果与转录组学数据变化趋势基本一致，表明转录组数据有较高的可靠性。综上认为，在长日照条件下，BdFKF1基因会影响光周期途径相关基因的表达，同时过表达BdFKF1基因具有促进烟草植株开花的作用。 |
| 关键词: FKF1基因，光周期，烟草，二穗短柄草，转录组学 |
| DOI：10.11931/guihaia.gxzw202312031 |
| 分类号: |
| 基金项目:国家自然科学基金 (32060394)；贵州省基金项目（黔科合基础-ZK[2023]一般164）；贵州省科技计划项目（黔科合基础-ZK[2022]一般222， 黔农科青年基金（2023）19号）；贵州省科研机构创新能力建设专项（黔科合服企[2022]004）。 |
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| Transcriptomic analysis of tobacco flowering regulated by the BdFKF1 gene in Brachypodium distachyon |
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YANG Longjiao 1,LI Yajiao 2,CHEN Xi,MA Peijie 2,LUO Wenju1, CHEN Caijun 2,CHEN Ying2,LIU Xiaoxia2,WANG Xiaoli 2*
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（1. Department of Grass Science, College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Grass Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China)
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| Abstract: |
| FKF1 gene is known to be a blue light-responsive gene and an important factor in regulating flowering through the photoperiod pathway. To explore the molecular mechanism of the BdFKF1 gene in regulating flowering in tobacco plants through the photoperiod pathway, wild-type tobacco （SR1）and BdFKF1 gene-transformed tobacco plants （BdFKF1-OE）were used as materials. Transcriptomic sequencing and RT-qPCR validation were conducted to observe and record the flowering time in both materials. The results were as follows: （1） In the SR1 vs FKF1 group, a total of 472 differentially expressed genes were identified, with 248 upregulated genes and 224 downregulated genes. Among them, 15 differentially expressed genes were related to the photoperiod, with 8 upregulated genes and 7 downregulated genes. （2） GO enrichment analysis revealed that the differentially expressed genes were significantly enriched in pathways such as U5 snRNP、 oxidoreductase activity、 acting on paired donors and response to blue light. Among them, the differentially expressed genes related to the photoperiod were mainly enriched in pathways such as FK506 binding、photoperiodism,flowering、 photoperiodism 、phosphatidylethanolamine binding, macrolide binding and regulation of flower development. （3） KEGG enrichment analysis revealed that the differentially expressed genes were mainly enriched in pathways such as ABC transporters、 Protein processing in endoplasmic reticulum、 Cutin, suberine and wax biosynthesis and Circadian rhythm – plant pathways. Among them, the differentially expressed genes related to the photoperiod were mainly enriched in pathways such as Circadian rhythm – plant and Plant hormone signal transduction. （4） The observation and recording showed that the flowering time of BdFKF1-OE plants was advanced by 3.9 days compared to SR1. （5） Finally, the RT-qPCR results were consistent with the trend of changes in the transcriptomic data, indicating that the transcriptomic data had high reliability. In conclusion, under long-day conditions, the BdFKF1 gene can affect the expression of photoperiod pathway-related genes, and overexpression of the BdFKF1 gene promotes flowering in tobacco plants. |
| Key words: FKF1 gene, photoperiod, tobacco, Brachypodium distachyon, transcriptomics |
