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| 维管植物质体基因组多样性及其获取与应用研究进展 |
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陈丽琼1, 黎若竹1,2, 李鑫1,3, 姚昕1, 杨俊波4, 李德铢4, 郁文彬1,5*
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(1.中国科学院西双版纳热带植物园综合保护中心和云南省热带雨林与亚洲象保护重点实验室,云南 勐腊 666303； 2. 钦州市农业技术推广中心,广西 钦州 535000; 3. 奈良先端科学技术大学院大学,日本 奈良 6300192； 4.中国科学院昆明植物研究所中国西南野生生物种质资源库,昆明 650201； 5. 中国科学院东南亚生物多样性研究中心,云南 勐腊 666303)
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| 摘要: |
| 维管植物是地球生物多样性的重要组成部分，因此维管植物的系统分类、多样性起源和演化等问题一直备受关注。随着测序技术的发展，越来越多的维管植物质体基因组序列被解析和发表，为深入认识维管植物多样性的起源和演化提供了新的证据。该文旨在介绍维管植物质体基因组的基本特征和结构多样性，并以寄生植物为例简述质体基因组退化；回顾当前主流的质体基因组的测序技术及组装策略，并探讨获取标本DNA和特殊类群的质体基因组时需要注意的问题。此外，该文就质体基因组在系统发育和超级DNA条形码研究中面临的问题与挑战做了探讨，并提出了相应的应对建议。 |
| 关键词: 质体基因组演化，基因组结构变异，质体基因组获取，系统发育，超级条形码 |
| DOI：10.11931/guihaia.gxzw202312035 |
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| 基金项目:国家自然科学基金（31870196, 32371700）；云南省兴滇人才专项、中国科学院西部青年人才项目；云南省基础研究专项重大项目(202101BC070003)。 |
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| Plastid genome diversity of vascular plants and its acquisition and applications: a review |
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CHEN Liqiong1, LI Ruozhu1,2, LI Xin1,3, YAO Xin1, YANG Junbo4, LI Dezhu4, YU Wenbin1,5*
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(1. Center for Integrative Conservation and Yunnan Key Laboratory for the Conservation of Tropical Rainforests and Asian Elephants, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan, China; 2. Qinzhou Agricultural Technology Promotion Center, Qinzhou 535000, Guangxi; 3. Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara 6300192, Japan; 4. The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 5. Southeast Asia Biodiversity Research Institute, Chinese Academy of Science, Mengla 666303, Yunnan, China)
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| Abstract: |
| Vascular plants are crucial to global biodiversity, so their systematic taxonomy, origin and evolution are important topics. With the rapid development of sequencing technology, numerous vascular plant plastomes have been sequenced and published, offering significant insights into these issues. This study aims to present the basic characteristics and structural variations of vascular plant plastomes, as well as plastome degradation in parasitic plants. In addition, this review examines the main methods of plastome sequencing and assembly, highlighting important considerations for herbarium DNA and lineages with challenging plastome assemblies. Lastly, this study discusses the difficulties with applying plastomes to phylogenetic and super-barcode studies, offering suggestions to address these issues. |
| Key words: plastome evolution, genome structure variation, acquiring plastomes, phylogeny, super-barcodes |
