| 摘要: |
| 为探究鬼点灯（Bothriospermum zeylanicum）的抗结直肠癌活性成分，该研究采用ODS反相柱色谱、高压制备液相色谱等方法对乙酸乙酯部进行分离纯化，通过质谱及核磁共振等波谱分析鉴定化合物结构，并釆用CCK8法检测化合物对人结直肠癌细胞（SW620、HT-29）及人正常结肠上皮细胞（NCM460） 增殖的影响。结果表明：(1)从鬼点灯乙酸乙酯部共分离得到12个化合物，分别鉴定为富马酸（1）、原儿茶酸（2）、富马酸单甲酯（3）、3,4,α-三羟基甲基苯丙酸酯（4）、对羟基苯甲酸（5）、echiumin A（6）、echiumin B（7）、(+)-异落叶松脂素-2a-O-β-D-葡萄糖苷（8）、对香豆酰-α-L-鼠李吡喃糖苷（9）、对香豆酸（10）、(-)-5-甲氧基异落叶松脂醇-3α-O-β-D-葡萄糖苷（11）、rupestrin B（12），化合物1-12均为首次从鬼点灯中分离得到。（2）化合物8和化合物12对SW620细胞增殖具有一定抑制作用，其IC50分别为（9.34±1.10） μmol·L-1和（0.33±0.06） μmol·L-1，化合物12对HT-29细胞增殖具有一定的抑制作用，其IC50为（15.94±1.56） μmol·L-1。该研究结果丰富了鬼点灯的化学成分，进一步明确了其抗结直肠癌活性成分，为鬼点灯的药效开发与利用提供了参考。 |
| 关键词: 鬼点灯，化学成分，结构鉴定，抗结直肠癌活性，乙酸乙酯部 |
| DOI：10.11931/guihaia.gxzw202401033 |
| 分类号: |
| 基金项目:2022年度“宿迁英才”群英计划青年项目；宿迁市科技局自然科学基金青年科技人才项目（K202202）；金华市科技局青年人才基金项目（2023KR03）；广西科技基地和人才专项（桂科AD17195002）。 |
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| Chemical constituents and anti-colorectal cancer activity of Bothriospermum zeylanicum |
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ZHAO Xiaochao1, CHAI Ling2, LIN Xiao3*, SHANG Xun1, GE Zhumei1, WAN Guangwen1
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(1.Suqian Affiliated Hospital of Nanjing University of Chinese Medicine, Suqian 223800, Jiangsu, China; 2. Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standars, Nanning 530022, China; 3. Guangxi University of Traditional Chinese Medicine Institute of Marine Medicine, Nanning 530022, China)
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| Abstract: |
| In order to explore the anti-colorectal cancer activity of Bothriospermum zeylanicum, the compounds were isolated from the ethyl acetate fraction by ODS gel column chromatography, high-pressure preparative liquid chromatography and other methods. MS, NMR and other spectroscopic were used to identify the structure of the compounds. And then the effects of the compounds on the proliferation of human colorectal cancer cells ( SW620, HT-29 ) and human normal colon epithelial cells ( NCM460 ) were detected by CCK8 method. The results were follows: (1) A total of 12 compounds were identified from the ethyl acetate fraction of B. zeylanicum, including fumaric acid (1), protocatechuic acid (2), monomethyl fumarate (3), 3,4, α-trihydroxymethylphenyl propionate (4), p-hydroxybenzoic acid (5), echiumin A (6), echiumin B (7), (+)-isolariciresinol-2a-O-β-D-glucopyranoside(8), p-coumaroyl-α-L- rhamnopyranoside (9), p-coumaric acid (10), (-)-5-methoxyisolariciresinol-3α-O-β-D-glucopyranoside (11), rupestrin B (12). Compounds 1-12 were isolated from B. zeylanicum for the first time. (2) Compounds 8 and 12 showed a certain inhibitory effect on the proliferation of SW620 cells, with IC50 values of （9.34±1.10）μmol·L-1 and （0.33±0.06） μmol·L-1, respectively. Compound 12 showed a certain inhibitory effect on the proliferation of HT-29 cells, with IC50 value of （15.94±1.56） μmol·L-1. This finding enriched the chemical composition of B. zeylanicum, and it was further clarified its anti-colorectal cancer activity ingredients. which provided a reference for the development and utilization of B. zeylanicum. |
| Key words: Bothriospermum zeylanicum, chemical constituents, structure identification, anti-colorectal cancer activity, ethyl acetate fraction |
