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| 基于FUNGuild的山药腐烂块茎真菌群落研究及潜在病原真菌的分离鉴定 |
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展莉平1, 任雪洋1, 张笑一1, 王瑞飞1,2,孔盈利1, 王强1,2, 李明军1, 杨清香1,2*,
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1.河南师范大学 生命科学学院,河南 新乡 453007；2. 河南省农业微生物生态与技术国际联合实验室
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| 摘要: |
| 为探讨山药腐烂块茎的真菌群落特征及主要病原，该研究采用ITS扩增子测序技术和FUNGuild分析阐明了其真菌群落组成、网络特征及生态功能类群，并对潜在病原真菌进行了分离鉴定。结果表明：（1）山药腐烂块茎中的优势菌门为子囊菌门（Ascomycota），优势属包括青霉属（Penicillium）、炭疽菌属（Colletotrichum）、镰刀菌属（Fusarium）、篮状菌属（Talaromyces）和粉红螺旋聚孢霉属（Clonostachys）等。真菌生态网络呈现明显的模块化和高比例的正相关边数（99.33%），真菌间趋向于极强的正向合作。（2）FUNGuild分析显示，10个真菌生态功能类群与山药块茎腐烂高度相关。其中，凋落物腐生-未命名腐生-木腐菌（dung saprotroph-undefined saprotroph-wood saprotroph）和内生-植物病原真菌（endophyte-plant pathogen）的相对丰度分别达到33.74%和23.64%，其代表性属分别为青霉和炭疽属。除此之外，与植物病原和木腐菌同时相关的生态功能类群共有3个，总相对丰度达到13.67%，代表性属为镰刀菌属。进一步Trait分析表明，青霉和镰刀菌等7属真菌很可能与山药块茎腐烂密切相关。（3）共分离鉴定真菌22株，隶属于6个属，包括镰刀菌属（9株）、青霉属（5株）和曲霉属（Aspergillus，4株）等。该研究结果为阐明山药块茎腐烂的发病规律、定向使用农药及筛选生防菌提供了很好的参考。 |
| 关键词: 山药，扩增子测序，真菌群落，FUNGuild，生态功能类群，植物病原真菌 |
| DOI：10.11931/guihaia.gxzw202401035 |
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| 基金项目:基金项目：国家现代农业产业技术体系（CARS-21）；中原科技创新领军人才（224200510011）；河南省高等学校重点科研项目（22B180006，20A180015）；河南省创新重点项目（202310476022）。 |
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| FUNGuild-based study of fungal community, and isolation and identification of potential pathogenic fungi in yam (Dioscorea opposita L.) rotting tubers |
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ZHNA Liping1, REN Xueyang1, ZHANG Xiaoyi1, WANG Ruifei1,2, KONG Yingli1, WANG Qiang1,2, LI Mingjun1, YANG Qingxiang1,2*,
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1. College of Life Sciences, Henan Normal University, Xinxiang 453007, China; 2. Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology, Xinxiang 453007, China
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| Abstract: |
| To investigate the characteristics of the fungal community and potential key pathogens, ITS rDNA gene amplicon sequencing technology and FUNGuild analysis were used to study the fungal community composition, network characteristics and ecological function groups in rotting tubers of Dioscorea. sp. Additionally, potential pathogenic fungi were isolated and identified. The results were as follows: ( 1 ) In rotting tubers of Dioscorea sp., the dominant phylum was Ascomycota, and prevalent fungal genera included Penicillium, Colletotrichum, Fusarium, Talaromyces, Clonostachys, etc. The fungal molecular ecological network exhibited a clear modular structure with a high ratio of positive correlation edges (99.33%), suggesting that positive cooperation was strengthen between different fungi. ( 2 ) FUNGuild analysis indicated that 10 guilds were highly correlated with yam tuber rot. Among these guilds, the relative abundances of dung saprotroph-undefined saprotroph-wood saprotroph and endophyte-plant pathogen were 33.74% and 23.64%, respectively, and the representative genera were Penicillium and Colletotrichum, respectively. Additionally, three guilds were related to both plant pathogen and wood saprotroph, and occupied 13.67% of the ecological functional groups. Moreover, Fusarium was representative genus of all three guilds. Traits analysis showed that some fungi (Penicillium, Fusarium, etc.) in the fungal community were probably invovled in yam tuber rot. ( 3 ) A total of 22 strains were isolated and belonged to 6 genera, including Fusarium ( 9 strains ), Penicillium ( 5 strains ) and Aspergillus ( 4 strains ), etc. This study provides an important reference for understanding the pathogenesis of tuber rot in Dioscorea sp., and for the selection of appropriate pesticides and biocontrol microbes. |
| Key words: Dioscorea sp, amplicion sequencing, fungal community, FUNGuild, ecological functional groups, plant pathogenic fungi |
