| 引用本文: | 李 泽, 陈 淼, 周 利, 孙梦珊, 向 维,
马 杰, 郑思乡, 曾建国, 李玉帆.龙牙百合远缘杂交后代鉴定及枯萎病抗性评价[J].广西植物,2024,44(12):2172-2186.[点击复制] |
| LI Ze, CHEN Miao, ZHOU Li, SUN Mengshan, XIANG Wei,
MA Jie, ZHENG Sixiang, ZEN Jianguo, LI Yufan .Identification and Fusarium wilt resistance evaluation of distant hybrid offspring of Lilium brownii var. viridulum[J].Guihaia,2024,44(12):2172-2186.[点击复制] |
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| 龙牙百合远缘杂交后代鉴定及枯萎病抗性评价 |
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李 泽1,2, 陈 淼1,2, 周 利4, 孙梦珊4, 向 维1,
马 杰5, 郑思乡4, 曾建国3, 李玉帆1,2*
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1. 湖南农业大学 园艺学院, 长沙 410128;2. 湖南省中亚热带优质花木繁育与利用工程研究中心, 长沙 410128;3. 湖南农业大学
动物医学院, 长沙 410128;4. 湖南省农业环境生态研究所, 长沙 410128;5. 湖南省棉花科学研究所, 湖南 常德 415000
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| 摘要: |
| 龙牙百合(Lilium brownii var. viridulum)在栽培过程中容易感染枯萎病,而观赏百合中的部分品种具有较强的枯萎病抗性。为了培育抗枯萎病的龙牙百合杂交后代,该文选择抗病性强的观赏百合品种与龙牙百合分别进行正交和反交,通过SSR分子标记鉴定杂种的真实性,并对杂交后代的枯萎病抗性进行鉴定。结果表明:(1)‘Black stone'等亚洲百合杂种系品种枯萎病抗病性较强,而龙牙百合、‘Pink planet'等品种枯萎病抗性最弱。(2)将龙牙百合作母本的正交组合共获得28个蒴果,最终获得3株龙牙百合 × ‘Black stone'的杂种苗; 将龙牙百合作父本的反交组合共获得74个蒴果,最终获得1株‘Strawberry event'×龙牙百合的杂种苗。(3)SSR分子标记鉴定结果显示,龙牙百合 × ‘Black stone'的3个杂种株系为真实杂种,‘Strawberry event'×龙牙百合的1个杂种株系为假杂种。(4)所获得的3个龙牙百合 × ‘Black stone'杂种株系枯萎病抗性均为中抗。该研究结果为龙牙百合与观赏百合的远缘杂交提供了技术参考,也为后期培育枯萎病抗性强的百合新品种提供了材料基础。 |
| 关键词: 龙牙百合, 远缘杂交, 枯萎病抗性, 胚珠培养, SSR分子标记 |
| DOI：10.11931/guihaia.gxzw202401061 |
| 分类号:Q945.8 |
| 文章编号:1000-3142(2024)12-2172-15 |
| 基金项目:湖南省科学技术厅种业创新项目(2021NK1005); 湖南省自然科学基金(2023JJ30292)。 |
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| Identification and Fusarium wilt resistance evaluation of distant hybrid offspring of Lilium brownii var. viridulum |
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LI Ze1,2, CHEN Miao1,2, ZHOU Li4, SUN Mengshan 4, XIANG Wei1,
MA Jie5, ZHENG Sixiang 4, ZEN Jianguo 3, LI Yufan 1,2*
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1. College of Horticulture, Hunan Agricultural University, Changsha 410128, China;2. Hunan Mid-subtropical Quality Plant Breeding and
Utilization Engineering Technology Research Center, Changsha 410128, China;3. College of Veterinary Medicine, Hunan Agricultural
University, Changsha 410128, China;4. Hunan Institute of Agricultural Environmental Ecology, Changsha 410128,
China;5. Hunan Cotton Research Institute, Changde 415000, Hunan, China
1. College of Horticulture, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Mid-subtropical Quality Plant Breeding and
Utilization Engineering Technology Research Center, Changsha 410128, China; 3. College of Veterinary Medicine, Hunan Agricultural
University, Changsha 410128, China; 4. Hunan Institute of Agricultural Environmental Ecology, Changsha 410128,
China; 5. Hunan Cotton Research Institute, Changde 415000, Hunan, China
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| Abstract: |
| During the cultivation process of Lilium brownii var. viridulum, it is easy to be infected with wilt disease, while some varieties of ornamental lilies have strong resistance to wilt disease. The study aimed to create hybrids with improved resistance to wilt disease by crossbreeding Lilium brownii var. viridulum with various ornamental lily varieties. Disease-resistant ornamental lily cultivars were specifically chosen for reciprocal crosses with Lilium brownii var. viridulum. The authenticity of the hybrids was confirmed through SSR molecular markers, and the resistance of the hybrid offspring to wilt disease was subsequently assessed. The results were as follows:(1)Lilium Asiatic hybrids such as ‘Black stone' had strong resistance to Fusarium wilt, while Lilium brownii var. viridulum and ‘Pink planet' had the weakest resistance to Fusarium wilt.(2)A total of 28 capsules were obtained from the orthogonal combination using Lilium brownii var. viridulum as the maternal parent, and 3 Lilium brownii var. viridulum × ‘Black stone' hybrid seedlings were finally obtained. The cross combination of Lilium brownii var. viridulum as the male parent obtained a total of 74 capsules, and ultimately obtained a hybrid seedling of ‘Strawberry event' × Lilium brownii var. viridulum.(3)SSR molecular marker identification results showed that 3 hybrid seedlings of Lilium brownii var. viridulum × ‘Black stone' were real hybrids, while 1 hybrid seedlings of ‘Strawberry event' × Lilium brownii var. viridulum was a false hybrid.(4)The 3 Lilium brownii var. viridulum × ‘Black stone' hybrid seedlings obtained had moderate resistance to Fusarium wilt. This study provides a reference for the distant hybridization of Lilium brownii var. viridulum and ornamental lilies, and proves that Lilium brownii var. viridulum hybrids with Fusarium wilt resistance can be obtained through hybridization. This study provides technical guidance for the distant hybridization of Lilium brownii var. viridulum with ornamental lily varieties, laying the groundwork for the breeding of new lily varieties with enhanced resistance to wilt disease in the future. |
| Key words: Lilium brownii var. viridulum, distant hybridization, Fusarium wilt resistance, ovule culture, SSR molecular markers |
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