| 引用本文: | 陈 妮, 彭丽辉, 江海都, 杨 哲, 蒋 强, 仇 硕,
熊忠臣, 韦 霄, 柴胜丰.四种珍稀濒危石斛属植物光合特性及叶片显微结构的比较研究[J].广西植物,2025,45(1):1-14.[点击复制] |
| CHEN Ni, PENG Lihui, JIANG Haidu, YANG Zhe, JIANG Qiang,
QIU Shuo, XIONG Zhongchen, WEI Xiao, CHAI Shengfeng.Comparative study on the photosynthetic properties of four rare and endangered Dendrobium species[J].Guihaia,2025,45(1):1-14.[点击复制] |
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| 四种珍稀濒危石斛属植物光合特性及叶片显微结构的比较研究 |
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陈 妮1,2, 彭丽辉1, 江海都1, 杨 哲2, 蒋 强3, 仇 硕1,
熊忠臣1, 韦 霄1, 柴胜丰1*
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1. 广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质与资源持续利用重点实验室, 广西 桂林 541006;2. 桂林理工大学
旅游与风景园林学院, 广西 桂林 541006;3. 广西雅长兰科植物国家级自然保护区管理中心, 广西 百色 533209
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| 摘要: |
| 为探究珍稀濒危石斛属植物的光合特性及其叶片显微结构特征,该研究对滇桂石斛(Dendrobium scoriarum)、喇叭唇石斛(D. lituiflorum)、罗河石斛(D. lohohense)和钩状石斛(D. aduncum)4种石斛属植物的净光合速率(Pn)日变化、光响应曲线、CO2响应曲线、叶绿素含量和叶片显微结构等指标进行了测定。结果表明:(1)滇桂石斛和钩状石斛白天Pn为正,上午8:00—10:00出现最大峰值,夜间Pn低于白天,存在Pn为正的情况; 喇叭唇石斛和罗河石斛白天Pn日变化呈双峰曲线,白天Pn为正,夜间Pn为负值。(2)4种石斛属植物中,喇叭唇石斛的光合能力最强,罗河石斛的光适应范围最宽。(3)喇叭唇石斛的初始羧化效率(α)、潜在最大净光合速率(Amax)和光呼吸速率(Rp)显著性(P<0.05)大于其他3种石斛。(4)滇桂石斛和钩状石斛的叶片厚度(LT)大于喇叭唇石斛和罗河石斛,而气孔密度(SD)则呈现相反趋势。(5)喇叭唇石斛的叶绿素总量(Chl)、叶绿素a(Chla)和叶绿素b(Chlb)的含量均显著性(P<0.05)大于其他3种石斛。(6)叶肉厚度(MT)与最大净光合速率(Pmax)呈显著性(P<0.05)负相关; SD与Pmax呈极显著性(P<0.01)正相关,与Amax呈显著性(P<0.05)正相关; Chl总量与Pmax、Amax呈显著性(P<0.05)正相关。综上认为,较厚的叶肉组织和低气孔密度可能是4种石斛属植物应对弱光环境以及附生于树干或生长于岩石缺水环境的适应机制,叶肉厚度、气孔密度及叶绿素总含量是影响4种石斛光合能力大小的重要因素,在引种栽培过程中营造适宜的光照环境、适当增加CO2浓度有利于4种石斛属植物的生长。该研究结果为石斛属植物种质资源保育和引种栽培提供了参考。 |
| 关键词: 石斛属, 光合特性, 景天酸代谢(CAM), 叶片显微结构, 叶绿素含量 |
| DOI：10.11931/guihaia.gxzw202406018 |
| 分类号:Q945 |
| 文章编号:1000-3142(2025)01-0001-14 |
| 基金项目:国家重点研发计划项目(2022YFF1300700); 广西林业局项目(2024LYKJ01); 广西林业科技推广示范项目(2023LYKJ03, [2022]GT23); 广西植物功能物质与资源持续利用重点实验室项目(ZRJJ2023-6)。 |
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| Comparative study on the photosynthetic properties of four rare and endangered Dendrobium species |
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CHEN Ni1,2, PENG Lihui1 , JIANG Haidu1, YANG Zhe1,2, JIANG Qiang3,
QIU Shuo1, XIONG Zhongchen1, WEI Xiao1, CHAI Shengfeng1*
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1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences/ Guangxi Key Laboratory of
Plant Functional Phytochemicals and Sustainable Utilization, Guilin 541006, Guangxi, China;2. College of Tourism and
Landscape Architecture, Guilin University of Technology, Guilin 541006, Guangxi, China;3. Yachang Orchid
National Nature Reserve Management Center, Baise 533209, Guangxi, China
1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences/ Guangxi Key Laboratory of
Plant Functional Phytochemicals and Sustainable Utilization, Guilin 541006, Guangxi, China; 2. College of Tourism and
Landscape Architecture, Guilin University of Technology, Guilin 541006, Guangxi, China; 3. Yachang Orchid
National Nature Reserve Management Center, Baise 533209, Guangxi, China
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| Abstract: |
| This study was carried out to determine the daily change of net photosynthetic rate(Pn), light response curve, CO2 response curve, leaf chlorophyll content and leaf microstructure of four Dendrobium species, including D. scoriarum, D. lituiflorum, D. lohohense, and D. aduncum. The purpose of this investigation was to learn more about the photosynthetic characteristics of Dendrobium species. The results were as follows:(1)Dendrobium scoriarum and D. aduncum had positive Pn during the daytime, with the maximum peak at 8:00—10:00 a.m., and the nighttime Pn was lower than that during the daytime, and there was positive Pn. Dendrobium lituiflorum and D. lohohense showed a bimodal curve of Pn during the daytime, with positive Pn during the daytime and negative Pn during the nighttime.(2)Among the four species of Dendrobium, D. lituiflorum had the strongest photosynthetic capacity, while D. lohohense had the widest light adaptation range.(3)The initial carboxylation efficiency(α), potential maximum net photosynthetic rate(Amax)and photorespiration rate(Rp)of D. lituiflorum were significantly(P<0.05)greater than those of the other three Dendrobium species.(4)The leaf thickness(LT)of D. aduncum and D. scoriarum were greater than those of D. lituiflorum and D. lohohense, while stomatal density(SD)showed an opposite trend.(5)The total chlorophyll content(Chl), chlorophyll a(Chla), and chlorophyll b(Chlb)of D. lituiflorum were significantly(P<0.05)higher than those of the other three Dendrobium species. (6)The mesophyll thickness(MT)was significantly(P<0.05)negatively correlated with maximum net photosynthetic rate(Pmax); SD was highly significant(P<0.01)positively correlated with Pmax, and significantly(P<0.05)positively correlated with potential maximum net photosynthetic rate(Amax); total chlorophyll content(Chl)was significantly(P<0.05)positively correlated with Pmax and Amax. In conclusion, the four Dendrobium species may respond to low light levels, being affixed to tree trunks, or growing in stony, water-deficient settings by developing larger leaf pulp tissue and low stomatal density. The four Dendrobium species' total chlorophyll concentration, stomatal density, and leaf pulp thickness are significant determinants of their photosynthetic potential. For the four Dendrobium species to flourish during introduction and cultivation, it is helpful to establish a proper light environment and raise the CO2 content adequately. The findings of this research can serve as a reference for the introduction and the preservation of genetic resources. |
| Key words: Dendrobium, photosynthetic properties, crassulacean acid metabolism(CAM), leaf microstructure, chlorophyll content |
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