| 引用本文: | 廖晓童, 张台民, 李卫锦, 李单凤, 杨 勇, 陈光程, 张 颖.海滨猫尾木叶绿体基因组特征及编码基因psbB对冷胁迫的响应[J].广西植物,2025,45(1):58-68.[点击复制] |
| LIAO Xiaotong, ZHANG Taimin, LI Weijin, LI Danfeng, YANG Yong,
CHEN Guangcheng, ZHANG Ying.Characteristics of chloroplast genome and response ofpsbB gene of Dolichandrone spathacea under cold stress[J].Guihaia,2025,45(1):58-68.[点击复制] |
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| 海滨猫尾木叶绿体基因组特征及编码基因psbB对冷胁迫的响应 |
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廖晓童1, 张台民1, 李卫锦1, 李单凤1, 杨 勇2, 陈光程1, 张 颖1*
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1. 岭南师范学院 生命科学与技术学院,红树林生态系统保护与修复重点实验室, 红树林珍稀濒危物种保护与利用
工程技术研究中心, 广东 湛江 524048;2. 海南师范大学 生命科学学院, 热带岛屿生态学教育部
重点实验室, 海南省热带动植物生态学重点实验室, 海口 571158
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| 摘要: |
| 低温是濒危红树植物海滨猫尾木(Dolichandrone spathacea)生态修复的重要障碍,植物叶绿体及其编码的基因密切响应冷胁迫,解析叶绿体基因组特征及冷胁迫下编码基因psbB的分子响应规律有助于该物种的保护与修复。为揭示叶绿体基因组信息,该研究通过Illumina NovaSeq测序平台对海滨猫尾木总DNA进行测序、组装、注释和功能分析,以及为探究冷胁迫下psbB基因的变化规律,并且检测了海滨猫尾木幼苗在不同温度处理下的总叶绿素、丙二醛、脯氨酸、总酚、可溶性蛋白和可溶性糖等生理参数,同时利用荧光定量PCR实验检测了相应处理下叶片psbB基因的表达水平。结果表明:(1)海滨猫尾木叶绿体具有典型四分体结构,基因组大小为159 139 bp,GC含量为37.9%,编码133个基因。其中,包括蛋白质编码基因(88个),tRNA基因(37个)和rRNA基因(8个)。(2)在系统发育分析中,海滨猫尾木未能形成单系,同其他8种紫葳科植物聚为一束,支持率达100%。(3)冷胁迫下,海滨猫尾木幼苗叶片总叶绿素含量随着处理温度的降低呈下降趋势,而总酚、丙二醛、脯氨酸、可溶性蛋白和可溶性糖的含量以及叶绿体编码基因psbB的相对表达量均随着处理温度的降低呈上升趋势。综上认为,海滨猫尾木叶绿体基因组特征与其他紫葳科植物高度保守。在冷胁迫下,该植物总叶绿素合成受到抑制,渗透性调节物质的合成受到影响,同时编码基因psbB参与了植物冷胁迫下的分子调控。以上结果为提升濒危红树植物抗冷能力分子辅助育种提供了前期基础。 |
| 关键词: 海滨猫尾木, 叶绿体基因组, 系统发育分析, 冷胁迫, 生理指标, psbB基因 |
| DOI：10.11931/guihaia.gxzw202404050 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)01-0058-11 |
| 基金项目:海南省重点研发计划项目(ZDYF2021SHFZ259); 湛江市科技计划项目(2022A05013); 海南省院士创新平台科研专项(YSPTZX2022011)。 |
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| Characteristics of chloroplast genome and response ofpsbB gene of Dolichandrone spathacea under cold stress |
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LIAO Xiaotong1, ZHANG Taimin1, LI Weijin1, LI Danfeng1, YANG Yong2,
CHEN Guangcheng1, ZHANG Ying1*
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1. Life Science and Technology College, Key Laboratory of Conservation and Restoration of Mangrove Ecosystem, Engineering and Technological
Research in Protection and Utilization of Mangrove Rare and Endangered Species, Lingnan Normal University, Zhanjiang 524048, Guangdong,
China;2. College of Life Sciences, Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of
Tropical Animal and Plant Ecology of Hainan Province, Hainan Normal University, Haikou 571158, China
1. Life Science and Technology College, Key Laboratory of Conservation and Restoration of Mangrove Ecosystem, Engineering and Technological
Research in Protection and Utilization of Mangrove Rare and Endangered Species, Lingnan Normal University, Zhanjiang 524048, Guangdong,
China; 2. College of Life Sciences, Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of
Tropical Animal and Plant Ecology of Hainan Province, Hainan Normal University, Haikou 571158, China
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| Abstract: |
| Low temperature is an important obstacle to the ecological restoration of endangered mangrove plant Dolichandrone spathacea. Plant chloroplasts and their coding genes closely respond to cold stress. Analyzing chloroplast genome characteristics and understanding the molecular response patterns of psbB genes under cold stress can help protect and restore this species. To reveal the chloroplast genome information, this study sequenced the total DNA of D. spathacea using the Illumina NovaSeq sequencing platform, and then annotated and functionally analyzed the assembled chloroplast genome. In the meantime, the changes of psbB gene in D. spathacea seedlings under different temperatures were investigated, and physiological parameters such as total chlorophyll, malondialdehyde, proline, total phenols, soluble protein, and soluble sugar were detected in this study. Further the expression value of psbB gene was detected using fluorescence quantitative PCR experiment. The results were as follows:(1)The chloroplast genome of D. spathacea exhibited a typical tetrad structure with the length of 159 139 bp, and the GC content of 37.9%. It encoded a total of 133 genes, including protein-coding genes(88), tRNA genes(37)and rRNA genes(8).(2)Phylogenetic analysis revealed that D. spathacea did not form a monophyletic group but instead clustered with 8 other Bignoniaceae species, with a support rate of 100%.(3)Under cold stress, the contents of total chlorophyll in the leaves of the D. spathacea seedlings decreased with the decrease of treatment temperature, while the contents of total phenols, malondialdehyde, proline, soluble protein and soluble sugar and relative expression values of psbB gene in the seedling leaves increased with the decrease of treatment temperature. In summary, the chloroplast genome characteristic of D. spathacea is highly conservative in Bignoniaceae family. Under cold stress, the total chlorophyll synthesis of the plant is inhibited, and the synthesis of osmoregulatory substances is affected. At the same time, the psbB gene is involved in molecular regulation under plant cold stress. The above results provide a preliminary basis for molecular assisted breeding to improve the cold resistance of endangered mangrove plants. |
| Key words: Dolichandrone spathacea, chloroplast genome, phylogenesis analysis, cold stress, physiological indicators, psbB gene |
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