| 引用本文: | 顾元钦, 郑健云, 黄 瑾, 胡静雯, 丁 妹, 杨 帆, 杜 勤.丛枝菌根真菌对穿心莲生长及有效成分的影响[J].广西植物,2025,45(2):377-388.[点击复制] |
| GU Yuanqin, ZHENG Jianyun, HUANG Jin, HU Jingwen, DING Mei, YANG Fan, DU Qin.Effects of arbuscular mycorrhizal fungi on growth and active ingredients of Andrographis paniculata[J].Guihaia,2025,45(2):377-388.[点击复制] |
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| 丛枝菌根真菌对穿心莲生长及有效成分的影响 |
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顾元钦, 郑健云, 黄 瑾, 胡静雯, 丁 妹, 杨 帆, 杜 勤*
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广州中医药大学 中药学院, 广州 510006
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| 摘要: |
| 为探究不同丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对穿心莲植株生长及有效成分含量的影响,该研究采用盆栽实验,对穿心莲植株进行单一接种摩西斗管囊霉(Funneliformis mosseaes, FM)和地表多样孢囊霉(Diversispora versiformis, DV)处理,在接种后的30、60、90、120 d分别测定穿心莲幼苗的生长指标、生理生化指标和有效成分含量。结果表明:(1)2种AMF均能与穿心莲良好共生,在接种120 d时,FM、DV侵染率分别为76.76%和90.09%,DV侵染能力较强。(2)接种AMF显著增加了穿心莲的株高、叶片数、茎基粗、叶面积、地上鲜重,其中DV组分别提高了35.59%、54.79%、38.94%、23.44%、37.51%。(3)接种AMF的2个处理组根表面积、根体积、根尖数、根鲜重均显著高于对照组,其中DV组分别提高了28.43%、26.82%、18.54%、68.25%。(4)接种AMF的2个处理组丙二醛含量均显著低于对照组,超氧化物歧化酶活性、过氧化物酶活性、过氧化氢酶活性和根系活力显著高于对照组,其中DV组丙二醛含量降低了18.87%,其余生理生化指标分别提高了70.77%、12.51%、24.78%、48.91%。(5)接种AMF的2个处理组穿心莲内酯、新穿心莲内酯、14-去氧穿心莲内酯、脱水穿心莲内酯含量均显著高于对照组,其中DV组地上部分有效成分含量分别提高了20.82%、98.64%、65.96%、61.57%。综上认为,接种AMF可促进穿心莲植株根系发育,进而促进植株营养生长,提高采收部位生物量及植株有效成分含量的积累,其中地表多样孢囊霉促进效果较佳。 |
| 关键词: 丛枝菌根真菌, 穿心莲, 根系活力, 穿心莲内酯, 药用植物 |
| DOI：10.11931/guihaia.gxzw202405001 |
| 分类号:Q945 |
| 文章编号:1000-3142(2025)02-0377-12 |
| 基金项目:广东省科技特派员项目(KTP20200137); 广州市重点研发和社会发展科技计划项目(2023B03J1273)。 |
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| Effects of arbuscular mycorrhizal fungi on growth and active ingredients of Andrographis paniculata |
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GU Yuanqin, ZHENG Jianyun, HUANG Jin, HU Jingwen, DING Mei, YANG Fan, DU Qin*
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College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
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| Abstract: |
| To study the effects of different arbuscular mycorrhizal fungi(AMF)on the growth and active ingredient contents of Andrographis paniculata, this study used a pot experiment in which A. paniculata was singly inoculated with Funneliformes mosseae(FM)and Diversespora diversiformis(DV)treatments, and their growth indexes, root morphology, physiological and biochemical indexes, and active ingredient contents were determined after inoculation for 30, 60, 90, and 120 d. The results were as follows:(1)Both AMFs could be well symbiotic with Andrographis paniculata, and at 120 d of inoculation, the infection rates of FM and DV were 76.76% and 90.09%, respectively, and the infection ability of DV was stronger.(2)Inoculation with AMF significantly increased the plant height, number of leaves, stem thickness, leaf area, and aboveground fresh weight of Andrographis paniculata, including 35.59%, 54.79%, 38.94%, 23.44%, and 37.51% in the DV group, respectively.(3)Root surface area, root volume, root tip and root fresh weight of the two treatment groups inoculated with AMF were significantly higher than those of the control group, in which the DV group increased by 28.43%, 26.82%, 18.54%, and 68.25%, respectively.(4)The malondialdehyde contents were significantly lower than those of the control group in both AMF-inoculated groups, and the activities of superoxide dismutase, peroxidase, catalase and root activity were significantly higher than those in the control group, in which the malondialdehyde content of the DV group was reduced by 18.87%, and the rest of physiological and biochemical indexes were increased by 70.77%, 12.51%, 24.78% and 48.91%, respectively.(5)The contents of andrographolide, neoandrographolide, 14-deoxyandrographolide, and dehydrated andrographolide in both treatment groups inoculated with AMF were significantly higher than those in the control group, and the contents of the aboveground active ingredients in the DV group were increased by 20.82%, 98.64%, 65.96%, and 61.57%, respectively. In conclusion, the inoculation of AMF can promote the development of root system of A. paniculata and then promote the nutritional growth of the plant, improve the biomass of harvested parts and the accumulation of active ingredient contents of the plant, among which the effect of Diversespora diversiformis promotion is better. |
| Key words: arbuscular mycorrhizal fungi, Andrographis paniculata, root activity, andrographolide, medicinal plant |
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