| 引用本文: | 李 晶, 邹碧群, 曾思文, 王亚凤, 阳丙媛, 李桂勤, 刘章彬,
黄永林, 郭伦发, 葛 利, 何瑞杰, 杨克迪.羊开口化学成分及其抑制一氧化氮生成活性的研究[J].广西植物,2025,45(10):1787-1798.[点击复制] |
| LI Jing, ZOU Biqun, ZENG Siwen, WANG Yafeng, YANG Bingyuan, LI Guiqin,
LIU Zhangbin, HUANG Yonglin, GUO Lunfa, GE Li, HE Ruijie, YANG Kedi.Melastoma normale and their nitric oxide inhibitory activities[J].Guihaia,2025,45(10):1787-1798.[点击复制] |
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| 羊开口化学成分及其抑制一氧化氮生成活性的研究 |
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李 晶1,2, 邹碧群2,3, 曾思文2,4, 王亚凤2, 阳丙媛2, 李桂勤2, 刘章彬2,
黄永林2, 郭伦发2, 葛 利1, 何瑞杰2*, 杨克迪1,4*
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1. 广西大学 医学院, 南宁 530004;2. 广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质与资源持续利用重点实验室, 广西 桂林
541006;3. 桂林师范高等专科学校 化学与药学系, 广西 桂林 541199;4. 广西大学 化学化工学院, 南宁 530004
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| 摘要: |
| 为探明羊开口的抗炎活性成分,该研究采用Sephadex LH-20柱层析和反相高效液相色谱(RP-HPLC)等技术,对羊开口的乙醇提取物进行系统的分离与纯化,基于质谱(MS)和核磁共振(NMR)波谱数据的综合分析,鉴定了所得化合物的结构,并利用脂多糖(LPS)诱导小鼠巨噬细胞RAW 264.7释放一氧化氮(NO)的模型,评估了化合物的抗炎活性。结果表明:(1)从羊开口中分离并鉴定了19个化合物,分别为 1,6-二-O-没食子酰基-β-D-葡萄糖苷(1)、3,5-二甲氧基-4-羟基苯基丙三醇-9-O-β-D-吡喃葡萄糖苷(2)、表儿茶素没食子酸酯(3)、龙胆酸5-O-β-D-葡萄糖苷(4)、杨梅素-3-O-β-D-葡萄糖苷(5)、6-O-没食子酰基葡萄糖(6)、3'-O-甲基鞣花酸-4-O-α-L-鼠李糖苷(7)、3,3',4-三甲氧基鞣花酸-4'-O-α-L-鼠李糖苷(8)、3,3',4-O-三甲基鞣花酸-4'-O-芸香糖苷(9)、3,3'-O-二甲基鞣花酸-4-O-β-D-葡萄糖苷(10)、原儿茶酸(11)、原儿茶醛(12)、β-D-吡喃葡萄糖氧基苯乙腈(13)、1-O-苯甲酰肌醇(14)、2α-羟基乌苏酸(15)、熊果酸(16)、木栓酮(17)、α-香树脂醇(18)、harprogenin-28-β-D-glucopyranosylester(19)。除化合物6、10、18外,其余化合物均为首次从该种植物中分离得到。(2)化合物12对LPS诱导RAW 264.7细胞释放NO具有显著的抑制作用,其IC 50值为18.2 μmol·L-1。该研究为羊开口的抗炎作用的深入研究提供了科学依据。 |
| 关键词: 羊开口, 野牡丹属, 一氧化氮抑制活性, 化学成分, 结构鉴定 |
| DOI：10.11931/guihaia.gxzw202411053 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)10-1787-12 |
| 基金项目:国家自然科学基金(32060108,82060764); 广西自然科学基金(2018GXNSFAA294033,2018GXNSFAA281078,2023GXNSFAA02642); 广西科技重大专项(桂科AA18118015); 广西重点研发计划项目(桂科AB22035019); 桂北特色药资源研究中心科研项目(YJZX202104); 桂林市创新平台和人才计划项目(20210102-3); 广西植物功能物质与资源持续利用重点实验室开放基金(FPRU2022-5) |
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| Melastoma normale and their nitric oxide inhibitory activities |
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LI Jing1,2, ZOU Biqun2,3, ZENG Siwen2,4, WANG Yafeng2, YANG Bingyuan2, LI Guiqin2,
LIU Zhangbin2, HUANG Yonglin2, GUO Lunfa2, GE Li1, HE Ruijie2*, YANG Kedi1,4*
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Melastoma normale and their nitric
oxide inhibitory activities
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| Abstract: |
| To investigate the chemical constituents of Melastoma normale roots and their ant-inflammatory activities, the ethanol extracts of M. normale roots were isolated using techniques such as Sephadex LH-20 column chromatography and RP-HPLC. The structures of obtained compounds were determined using a comprehensive analysis of NMR and HRESIMS data. Additionally, the anti-inflammatory activity of the compound was evaluated by inducing the release of nitric oxide in RAW 264.7 cells using lipopolysaccharide(LPS). The results were as follows:(1)A total of 19 compounds were isolated and identified as 1,6-di-O-galloyl-β-D-glucose(1), syringylglycerol-9-O-β-D-glucopyranoside(2), epicatechin gallate(3), gentisic acid 5-O-β-D-glucoside(4), myricetin 3-O-β-D-glucopyranoside(5), 6-O-galloylglucose(6), 3-O-methylellagic acid 4'-O-rhamnopyranoside(7), ellagic acid 3,3',4-trimethoxy 4'-O-α-L-rhamnopyranoside(8), 3,3',4-tri-O-methyl-4'-O-rutinosylellagicacid(9), 3,3'-di-O-methylellagic acid-4'-O-glucoside(10), 3,4-dihydroxybenzoicacid(11), 3,4-dihydroxybenzaldehyde(12), β-D-glucopyranosyloxyphenylacetoniwile(13), 1-O-benzoyl-myo-inositol(14), 2α-hydroxyursolic acid(15), ursolic acid(16), friedelin(17), α-amyrin(18), and harprogenin-28-β-D-glucopyranosylester(19). Except for compounds 6, 10, 18, all others were obtained from this plant for the first time.(2)Compound 12 exhibited a significant inhibitory effect on LPS inducing NO release in RAW 264.7 cells with an IC 50 value of 18.2 μmol · L-1. This finding provides a scientific basis for further research on the anti-inflammatory effect of M. normale. |
| Key words: Melastoma normale roots, Melastoma L., nitric oxide inhibitory activities, chemical constituents, structural identification |
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