| 引用本文: | 黄 瑾, 陈烁晴, 丁 妹, 胡静雯, 杨 帆, 罗文荣, 杜 勤.穿心莲内生真菌Colletotrichum sp. AP-12培养条件优化及防治广藿香青枯病[J].广西植物,2025,45(10):1844-1856.[点击复制] |
| HUANG Jin, CHEN Shuoqing, DING Mei, HU Jingwen,
YANG Fan, LUO Wenrong, DU Qin.Optimization of culture conditions for Colletotrichum sp. AP-12, an endophytic fungus from Andrographis paniculata, and its control of bacterial wilt in Pogostemon cablin[J].Guihaia,2025,45(10):1844-1856.[点击复制] |
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| 穿心莲内生真菌Colletotrichum sp. AP-12培养条件优化及防治广藿香青枯病 |
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黄 瑾, 陈烁晴, 丁 妹, 胡静雯, 杨 帆, 罗文荣, 杜 勤*
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广州中医药大学 中药学院, 广州 510006
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| 摘要: |
| 针对当前生防研究在“病原菌-生防菌-宿主”三方互作机制研究不足的问题,为构建“发酵工艺优化-防效验证-生理调控”研究体系以系统解析三者的互作关系,该文以穿心莲内生真菌Colletotrichum sp. AP-12(以下简称AP-12)为研究对象,通过单因素试验结合正交试验优化AP-12发酵工艺,并采用盆栽试验检测其对广藿香青枯病的防治效果及对青枯菌胁迫下广藿香生理生化指标和有效成分含量的影响。结果表明:(1)菌株AP-12的最佳发酵工艺为果糖20 g·L-1、酵母粉20 g·L-1、pH 6.0、装液量400 mL、转速150 r·min-1、温度28 ℃、发酵时间8 d,优化后抑菌活性较初始条件提高了83.41%。(2)盆栽试验显示,AP-12发酵液及灭菌发酵液对广藿香青枯病的防治效果分别为31.45%和21.58%,证实其活性物质具有热稳定性。(3)AP-12处理延缓了青枯菌胁迫下广藿香叶片的叶绿素含量和含氮量的降低,降低了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量(P<0.05)。(4)AP-12处理缓解了青枯菌胁迫下广藿香叶片中广藿香酮含量及根系活力的下降(P<0.05)。综上认为,内生真菌AP-12通过优化发酵工艺显著提升了抑菌活性,并通过“抑菌-抗逆-提质”协同作用机制,增强了广藿香的抗氧化能力、减轻了氧化损伤、缓解了有效成分含量降低,显著提高了广藿香对青枯菌的抗性。该研究结果不仅为广藿香青枯病的生物防治提供了高效菌种资源,而且还为植物内生真菌在农业病害绿色防控中的应用提供了新的理论依据和技术支持。 |
| 关键词: 广藿香青枯病, 穿心莲内生真菌AP-12, 发酵工艺优化, 协同防治, 氧化防御 |
| DOI：10.11931/guihaia.gxzw202503026 |
| 分类号:Q945.8 |
| 文章编号:1000-3142(2025)10-1844-13 |
| 基金项目:广东省科技特派员项目(KTP20200137); 广州市重点研发和社会发展科技计划项目(2023B03J1273)。 |
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| Optimization of culture conditions for Colletotrichum sp. AP-12, an endophytic fungus from Andrographis paniculata, and its control of bacterial wilt in Pogostemon cablin |
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HUANG Jin, CHEN Shuoqing, DING Mei, HU Jingwen,
YANG Fan, LUO Wenrong, DU Qin*
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College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
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| Abstract: |
| To address the limited understanding of tripartite interactions among “pathogen-biocontrol agent-and host” in current biological control research, and to establish an integrated research framework of “fermentation optimization-efficacy evaluation-physiological regulation” to systematically elucidate their interplay endophytic fungus AP-12 from Andrographis paniculata was used as research object, its fermentation process was optimized through single-factor and orthogonal tests. The biocontrol efficacy of AP-12 against Ralstonia solanacearum-induced wilt in Pogostemon cablin was evaluated via pot experiments, alongside its effects on physiological, biochemical, and bioactive compound levels. The results were as follows:(1)Optimal fermentation conditions(20 g·L-1 fructose, 20 g·L-1 yeast powder, pH 6.0, 400 mL liquid filling volume, 150 r·min-1, 28 ℃, 8 d)enhanced AP-12's antibacterial activity by 83.41%.(2)AP-12 fermented broth and sterilized broth exhibited disease control efficiencies of 31.45% and 21.58%, respectively, confirming thermostability of active metabolites.(3)AP-12 treatment mitigated chlorophyll and nitrogen loss in Pogostemon cablin under pathogen stress, while reducing activities of catalase(CAT), peroxidase(POD), superoxide dismutase(SOD), and malondialdehyde(MDA)content(P<0.05).(4)AP-12 alleviated declines in pogostone content and root vitality(P<0.05)under pathogen stress. In conclusion, it is believed that the endophytic fungus AP-12 significantly enhanced the antibacterial effect by optimizing the fermentation process through the synergistic mechanism of “antibacterial-stress resistance-quality improvement”, enhanced the antioxidant capacity, reduced the oxidative damage, and alleviated the effective constituent, significantly enhanced resistance to bacterial blight of P. cablin. This study not only provides a high standard biocontrol agent for bacterial wilt in Pogostemon cablin, but also provide a new theoretical basis and technical support for the application of plant endophytic fungi in the green prevention and control of agricultural diseases. |
| Key words: bacterial wilt in Pogostemon cablin, endophytic fungus AP-12 from Andrographis paniculata, fermentation process optimization, synergistic biocontrol, oxidative defense mechanism |
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