| 引用本文: | 杜明阳, 马健芝, 多杰措, 罗天蓉, 熊辉岩, 段瑞君.蒿叶猪毛菜SaGPAT1基因克隆与表达分析[J].广西植物,2025,45(10):1882-1893.[点击复制] |
| DU Mingyang, MA Jianzhi, DUO Jiecuo, LUO Tianrong,
XIONG Huiyan, DUAN Ruijun.Cloning and expression analysis of SaGPAT1 gene from Salsola abrotanoides[J].Guihaia,2025,45(10):1882-1893.[点击复制] |
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| 蒿叶猪毛菜SaGPAT1基因克隆与表达分析 |
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杜明阳1, 马健芝 1, 多杰措2, 罗天蓉1, 熊辉岩2, 段瑞君1,2*
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1. 青海大学 生态环境工程学院, 西宁 810000;2. 青海大学 农牧学院, 西宁 810000
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| 摘要: |
| 甘油-3-磷酸酰基转移酶(glycerol-3-phosphate acyltransferase, GPAT)是植物细胞膜、种子油脂及表皮蜡质的重要组分,在植物生长发育及抗逆方面具有重要作用。为探究蒿叶猪毛菜GPAT基因在高原植物中的抗旱分子机制与表达模式,该研究以蒿叶猪毛菜为材料,通过 qRT-PCR 技术克隆了1个GPAT基因家族成员SaGPAT1基因,采用生物信息学分析及基因表达模式初步解释了其功能。结果表明:(1)克隆的SaGPAT1基因cDNA长度大小为1 648 bp,编码532个氨基酸,其蛋白定位于内质网并为稳定蛋白,主要由α螺旋和无规则卷曲组成且因其编码蛋白具有PlsC结构域,属于GPAT家族。(2)系统发育树分析表明SaGPAT1蛋白与藜麦、菠菜同源蛋白具有更近的亲缘关系,并且该蛋白与拟南芥中的AtGPAT2和AtGPAT3归于同一进化支。(3)对SaGPAT1启动子预测分析发现,其存在多个与植物生长发育、胁迫响应相关的多种顺式作用元件。(4)qRT-PCR结果表明,SaGPAT1基因在不同组织均有表达,但表达情况在各组织中表现不同(叶>茎>幼苗); 在干旱胁迫下,SaGPAT1基因上调,表达量显著增加。综上认为,SaGPAT1基因在蒿叶猪毛菜抗旱过程中可能参与了与干旱胁迫响应相关的调控机制。该研究为后续通过转化模式植物进一步明确该基因的具体功能提供一定参考。 |
| 关键词: 蒿叶猪毛菜, 甘油-3-磷酸酰基转移酶, 基因克隆, 干旱胁迫, 表达分析 |
| DOI：10.11931/guihaia.gxzw202404046 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)10-1882-12 |
| 基金项目:青海省中央引导地方科技发展资金计划项目(2023ZY003); 2023年度青海省“昆仑英才·高端创新创业人才”计划项目。 |
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| Cloning and expression analysis of SaGPAT1 gene from Salsola abrotanoides |
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DU Mingyang1, MA Jianzhi1, DUO Jiecuo2, LUO Tianrong1,
XIONG Huiyan2, DUAN Ruijun1,2*
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1.College of Eco-Environmental Engineering, Qinghai University, Xining 810000, China;2. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810000, China
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| Abstract: |
| Glycerol-3-phosphate acyltransferase(GPAT)is a crucial component of plant cell membranes, seed lipids, and epidermal waxes. It plays a significant role in plant growth, development, and stress resistance. To investigate the drought tolerance molecular mechanisms and expression patterns of the GPAT gene in the plateau plant Salsola abrotanoides, SaGPAT1, a member of the GPAT gene family, was cloned from S. abrotanoides using qRT-PCR. Its function was preliminarily interpreted through bioinformatics analysis and gene expression patterns. The results were as follows:(1)The cloned SaGPAT1 gene cDNA was 1 648 bp in length and encoded a protein of 532 amino acids. This study protein was localized in the endoplasmic reticulum, was stable, mainly consisted of α helix and random coil, and belonged to the GPAT family because it contained a PlsC structural domain.(2)Phylogenetic tree analysis showed that the SaGPAT1 protein was more closely related to those in Chenopodium quinoa and Spinacia oleracea and clustered in the same clade as AtGPAT2 and AtGPAT3 in Arabidopsis thaliana.(3)Predictive analysis of the SaGPAT1 promoter revealed several cis-acting elements related to plant growth, development, and stress response.(4)qRT-PCR results showed that the SaGPAT1 gene was expressed in different tissues, with the highest expression in leaves, followed by stems and seedlings. Under drought stress, the SaGPAT1 gene was upregulated, and its expression increased significantly. In conclusion, it is hypothesized that the SaGPAT1 gene might be involved in the regulatory mechanisms related to drought stress response in Salsola abrotanoides. This study provides a reference for further clarification of the gene's specific function in drought tolerance through transformation studies in model plants. |
| Key words: Salsola abrotanoides, glycerol-3-phosphate acyltransferase, gene cloning, drought stress, expression analysis |
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