| 引用本文: | 曾 坚, 张燕秋, 陈丽萍, 吴春来, 胡 伟.木薯MeHSF10基因克隆及表达分析[J].广西植物,2021,41(9):1576-1584.[点击复制] |
| ZENG Jian, ZHANG Yanqiu, CHEN Liping, WU Chunlai, HU Wei.Clone and expression analysis of MeHSF10 in Manihot esculenta[J].Guihaia,2021,41(9):1576-1584.[点击复制] |
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| 木薯MeHSF10基因克隆及表达分析 |
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曾 坚1, 张燕秋1, 陈丽萍1, 吴春来2, 胡 伟2*
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1.韶关学院 英东生命科学学院, 广东 韶关 512005;2. 中国热带农业科学院热带生物技术研究所, 海口 571101
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| 摘要: |
| 热激转录因子(heat shock transcription factor, HSF)是植物中重要的逆境调控因子。许多研究表明HSF可通过对信号通路下游的逆境相关基因进行调控而提高植物适应逆境的能力,如提高植株在干旱和氧化损伤等逆境中的耐受能力。为研究HSF在木薯抗逆和采后储存中的功能,该研究以木薯品种SC124为材料,通过RT-PCR技术从木薯叶片克隆得到一个木薯HSF家族基因,将其命名为MeHSF10。结果表明:(1)该基因全长为1 098 bp,编码365个氨基酸残基,蛋白质相对分子量为40.7 kD,理论等电点为8.15,蛋白的亚细胞定位预测为细胞核。蛋白质序列分析结果表明MeHSF10与麻风树JcHSF和橡胶树HbHSF的相似性最高,分别为80.31%和90.54%。MeHSF10基因的蛋白序列含有HSF蛋白家族的保守结构域,如DBD、HR-A Core、HR-B Core、插入序列和核定位信号(nuclear localization signal, NLS),表明MeHSF10基因编码的蛋白质属于HSFC家族成员。(2)为分析MeHSF10基因在木薯不同组织中的表达情况,对该基因在木薯11个组织中的表达进行分析,结果表明MeHSF10基因在木薯所有组织中都有表达,在叶片中的表达最高。(3)MeHSF10基因的启动子序列元件分析结果显示其含有脱落酸(abscisci acid, ABA)响应(ABA responsive motif)、干旱诱导(drought-induced motif)和光响应(light-responsive motif)等元件。(4)表达分析结果也表明,MeHSF10基因能被干旱和ABA处理显著诱导,MeHSF10基因在木薯块茎的采后生理性变质过程中也被显著诱导表达。综上结果表明,MeHSF10基因可能在转录水平参与ABA介导的木薯干旱胁迫响应和木薯块茎的采后生理性变质,这为进一步研究其在木薯抗逆和采后储存中的功能奠定基础。 |
| 关键词: 热激转录因子, MeHSF10, 非生物胁迫, 采后生理性变质(PPD), 表达分析 |
| DOI：10.11931/guihaia.gxzw202002041 |
| 分类号:Q943 |
| 文章编号:1000-3142(2021)09-1576-09 |
| 基金项目:国家自然科学基金(31901537); 韶关学院重点项目(SZ2018KJ05); 韶关市科技计划项目(2019sn087); 韶关学院博士启动项目(99000615); 广东省教育厅青年创新人才项目(2018KQNCX234)[Supported by the National Natural Science Foundation of China(31901537); Key Program of Shaoguan University(SZ2018KJ05); Science and Technology Planning Program of Shaoguan City(2019sn087); Doctoral Scientific Research Startup Foundation of Shaoguan University(99000615); Foundation for Young Innovation Talents of Education Office of Guangdong Province(2018KQNCX234)]。 |
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| Clone and expression analysis of MeHSF10 in Manihot esculenta |
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ZENG Jian1, ZHANG Yanqiu1, CHEN Liping1, WU Chunlai2, HU Wei2*
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1. Henry Fok College of Life Sciences, Shaoguan University, Shaoguan 512005, Guangdong, China;2. Institute of Tropical
Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
1. Henry Fok College of Life Sciences, Shaoguan University, Shaoguan 512005, Guangdong, China; 2. Institute of Tropical
Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
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| Abstract: |
| Heat shock transcription factor(HSF)is a key adverse regulatory factor in plants. Many studies have shown that HSFs can partake in the downstream of signal transduction pathways and regulate genes expression responsive for a variety of abiotic/biotic stresses, to improve the tolerance to high salinity and oxidative stress in plants. In order to analyze the function of HSF in stress tolerance and post-harvest storage of Manihot esculenta, M. esculenta cv. SC124 was used as cloning material. In this study, a HSF gene designated MeHSF10 was isolated from M. esculenta leaves through RT-PCR method. The results were as follows:(1)The full-length cDNA of MeHSF10 was 1 098 bp, encoded a polypeptide of 365 amino acid residues with a predicted relative molecular mass of 40.7 kD and an isoelectric point of 8.15. Subcellular localization of MeHSF10 was predicted as nuclear localization. Multiple protein sequence alignment showed that MeHSF10 shared a significant degree of sequence similarity with other HSF proteins in Jatropha curcas(80.31%)and Hevea brasiliensis(90.54%). The protein sequence of MeHSF10 contained conserved motifs of the HSF family, such as DNA Binding Domain(DBD), HR-A Core, HR-B Core, insert sequence, and nuclear localization sequence signal(NLS), these results suggested that MeHSF10 isolated from M. esculenta was a genuine member of the HSFC family.(2)In order to analyze the expression profiles of MeHSF10 gene in M. esculenta, the expression data of eleven M. esculenta tissues/organ types were analyzed, and the result showed that MeHSF10 gene expressed in all eleven M. esculenta tissues, and the highest expression of MeHSF10 was in leaf.(3)Results of promoter element analysis revealed that MeHSF10 contained drought-induced motif(MBS), ABA responsive motif(ABRE), and several light-responsive motifs.(4)The transcriptome data results also showed that MeHSF10 was upregulated by drought stress and ABA treatment. The expression of MeHSF10 was also induced in M. esculenta post-harvest physiological deterioration(PPD)process. All the above results indicate that MeHSF10 may be involved in ABA mediated drought stress response, and that MeHSF10 is also related with PPD and may operate mainly through ROS-regulated gene networks. Therefore, these results offered critical basic knowledge for future gene function analysis of MeHSF10 in stress tolerance and post-harvest storage of Manihot esculenta. |
| Key words: heat shock transcription factor(HSF), MeHSF10, abiotic stress, post-harvest physiological deterioration(PPD), expression analysis |
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