| 引用本文: | 刘保财, 陈菁瑛, 张武君, 刘剑超, 黄颖桢, 赵云青, 刘红跃.多花黄精种子萌发前后基因表达特征分析[J].广西植物,2023,43(11):2078-2090.[点击复制] |
| LIU Baocai, CHEN Jingying, ZHANG Wujun, LIU Jianchao,
HUANG Yingzhen, ZHAO Yunqing, LIU Hongyue.Analysis of gene expression characteristics before and after seed germination of Polygonatum cyrtonema [J].Guihaia,2023,43(11):2078-2090.[点击复制] |
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| 多花黄精种子萌发前后基因表达特征分析 |
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刘保财1,2, 陈菁瑛1,2*, 张武君1,2, 刘剑超3, 黄颖桢1,2, 赵云青1,2, 刘红跃 4
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1. 福建省农业科学院农业生物资源研究所, 福州 350003;2. 福建省农业科学院药用植物研究中心, 福州 350003;3. 福建省南平市邵武市农业农村局, 福建 邵武 354000;4. 福建省南平市邵武市林业局, 福建 邵武 354000
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| 摘要: |
| 多花黄精种子具有综合休眠现象,解析其种子萌发前后基因表达的变化,对探讨种子生理、打破休眠等具有重要作用。该研究基于高通量Illumina HiSeq 2500测序技术,对萌发过程的4个阶段进行转录组测序及生物信息分析,剖析萌发前后差异基因表达特征。结果表明:(1)通过de novo组装,分别获得388 231个Transcripts和178 319 个Unigenes。(2)萌发前后显著性差异表达的Unigenes有11 817个,其中上调的有6 405个,下调的有5 412个。(3)GO富集分析表明,上调与下调的差异基因均主要富集在生物过程和分子功能中,主要参与代谢过程、催化活性等。(4)KEGG富集分析表明,差异基因主要富集于核糖体、植物激素信号转导、淀粉和蔗糖代谢等通路中; 上调的差异基因中富集在核糖体231个、植物激素信号转导56个、淀粉和蔗糖代谢76个,下调的差异基因中富集在苯丙烷生物合成48个、植物激素信号转导48个、淀粉和蔗糖代谢48个; 40个差异基因参与了生长素通路中关键酶的合成; 淀粉和蔗糖代谢通路中,编码蔗糖磷酸合酶等基因下调,而编码糖原磷酸化酶等基因则上调。该研究结果初步明确了植物激素信号转导与淀粉和蔗糖代谢在多花黄精种子萌发前后起着关键作用,并解析了这两条通路上参与的关键基因,为多花黄精种子的生理、繁殖及育种等深入研究提供了参考。 |
| 关键词: 多花黄精, 种子, 萌发前后, 基因, 表达特征 |
| DOI：10.11931/guihaia.gxzw202210040 |
| 分类号:Q943 |
| 文章编号:1000-3142(2023)11-2078-13 |
| 基金项目:福建省人民政府协同创新工程项目(XTCXGC2021003); 南平市科技局项目(N2020Y004); 福建省农业科学院科技创新团队(CXTD2021014-2); 福建省农业科学院药用植物种苗繁育工程化实验室项目(2015GCH-6)。 |
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| Analysis of gene expression characteristics before and after seed germination of Polygonatum cyrtonema |
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LIU Baocai1,2, CHEN Jingying1,2*, ZHANG Wujun1,2, LIU Jianchao3,
HUANG Yingzhen1,2, ZHAO Yunqing1,2, LIU Hongyue4
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1. Institute of Agricultural Bioresource, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;2. Research Center for Medicinal Plant,
Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;3. Agricultural and Rural Bureau of Shaowu City, Nanping City, Fujian Province,
Shaowu 354000, Fujian, China;4. Forestry Bureau of Shaowu City, Nanping City, Fujian Province, Shaowu 354000, Fujian, China
1. Institute of Agricultural Bioresource, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2. Research Center for Medicinal Plant,
Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 3. Agricultural and Rural Bureau of Shaowu City, Nanping City, Fujian Province,
Shaowu 354000, Fujian, China; 4. Forestry Bureau of Shaowu City, Nanping City, Fujian Province, Shaowu 354000, Fujian, China
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| Abstract: |
| Polygonatum cyrtonema seeds have a comprehensive dormancy phenomenon, and analyzing the changes of key genes before and after seeds germination plays an important role in exploring seed physiology and breaking dormancy. Based on high-throughput sequencing technology(Illumina Hiseq 2500), the transcriptome sequencing and bioinformatics analysis were carried out in four stages of P. cyrtonema seed germination with three biological replicates. The results were as follows:(1)388 231 Transcripts and 178 319 Unigenes were obtained by de novo assembly.(2)Among the 11 817 Unigenes with significant differences before and after germination, 6 405 were up-regulated and 5 412 were down-regulated.(3)Significant difference genes were enriched and analyzed in the GO enrichment analysis, and the differential expression up- and down-regulation Uningenes were main enriched in biological process and molecular function, which were mainly involved in metabolic processes and catalytic activities.(4)KEGG significant enrichment indicated that the differential expression genes were mainly enriched in ribosome, plant hormone signal transduction, starch and sucrose metabolism and other pathways; the up-regulated differential genes were mainly enriched in ribosome 231, plant hormone signal transduction 56, starch and sucrose metabolism 76 and others; the down-regulated differential genes were mainly enriched in phenylpropanoid biosynthesis 48 plant hormone signal transduction 48, starch and sucrose metabolism 48, and other pathways; a total of 40 differential genes were involved in the synthesis of auxin pathway key enzymes. Genes encoding sucrose phosphate synthase were down-regulated and genes encoding glycogen phosphorylase were up-regulated in starch and sucrose metabolic pathways. This paper preliminarily clarified that plant hormone signal transduction, starch and sucrose metabolism played a key role before and after the seeds germination of P. cyrtonema and analyzed the key genes involved in these two pathways. This study preliminarily clarifies that plant hormone signal transduction and starch and sucrose metabolism play a key role before and after the germination of P. cyrtonema seeds, and analyzes the key genes involved in these two pathways, which provides a reference for further research on the physiology, reproduction and breeding of P. cyrtonema seeds. |
| Key words: Polygonatum cyrtonema, seeds, before and after germination, genes, expression characteristics |
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