外源NO处理对四种桉树幼苗铝胁迫抗性的影响

刘 冰<sup>1; 2; 3; 4; 5</sup>; 郭荣琨<sup>1</sup>; 石茂鑫<sup>6</sup>; 罗义山<sup>1</sup>; 蒋丰璟<sup>1</sup>; 滕维超<sup>1; 2; 3; 4; 5*</sup>

引用本文:	刘冰, 郭荣琨, 石茂鑫, 罗义山, 蒋丰璟, 滕维超.外源NO处理对四种桉树幼苗铝胁迫抗性的影响[J].广西植物,2023,43(12):2269-2279.[点击复制]
	LIU Bing, GUO Rongkun, SHI Maoxin, LUO Yishan, JIANG Fengjing, TENG Weichao.Effects of exogenous NO treatment on aluminum stress resistance of four Eucalyptus seedlings[J].Guihaia,2023,43(12):2269-2279.[点击复制]

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外源NO处理对四种桉树幼苗铝胁迫抗性的影响
刘冰^1,2,3,4,5, 郭荣琨¹, 石茂鑫⁶, 罗义山¹, 蒋丰璟¹, 滕维超^1,2,3,4,5*
1. 广西大学林学院, 南宁 530004;2. 广西森林生态与保育重点实验室, 南宁 530004;3. 亚热带农业生物资源保护与利用国家重点实验室, 南宁 530004;4. 国家林草局中南速生材繁育重点实验室, 南宁 530004;5. 广西高等学校林业科学与工程重点实验室, 南宁 530004;6.6. 广西壮族自治区国有东门林场, 广西崇左 532108

摘要:

为探究外源一氧化氮(NO)对铝胁迫下桉树幼苗耐铝性的影响,该研究以4种3月生桉树幼苗(巨桉、尾叶桉、圆角桉、尾巨桉)为对象,将硝普钠(SNP)作为外源NO供体,采用水培法,研究不同浓度NO(0、50、100、200、400、800 μmol·L^-1)对120 mg·L^-1铝胁迫条件下桉树幼苗的ROS、抗氧化酶活性和有机渗透调节物质含量等生理指标的影响,并比较4种桉树在NO处理下的耐铝性差异。结果表明:(1)铝胁迫下添加适宜浓度外源NO(50 μmol·L^-1≤NO≤200 μmol·L^-1),可促使4种桉树提高可溶性糖和可溶性蛋白的含量、抗氧化酶(SOD、POD、CAT、APX)活性,清除体内的ROS和降低MDA的积累,提高抗铝性,但在高浓度的NO(≥800 μmol·L^-1)处理下桉树幼苗的抗氧化酶活性和渗透调节物质含量降低,表现出胁迫反应。(2)NO对于敏感型桉树的耐铝性有较强的提升作用,对耐受型桉树的耐铝性提升不明显,在NO的作用下4种桉树的抗铝性最终趋于一致。(3)SOD、MDA、CAT、O₂^-、可溶性蛋白和可溶性糖这些指标可作为评判桉树耐铝性强弱的关键指标。该研究结果为桉树耐铝种质资源的选择提供科学参考,为深入了解NO调控桉树种间耐铝性差异的机制奠定了基础。

关键词: 桉树, 外源NO, 铝胁迫, 生理指标, 主成分分析, 耐铝性

DOI：10.11931/guihaia.gxzw202210058

分类号:Q945

文章编号:1000-3142(2023)12-2269-11

基金项目:国家自然科学基金(31800530); 广西自然科学基金(2023GXNSFAA026485, 2018GXNSFBA281009)。

Effects of exogenous NO treatment on aluminum stress resistance of four Eucalyptus seedlings

LIU Bing^1,2,3,4,5, GUO Rongkun¹, SHI Maoxin⁶, LUO Yishan¹, JIANG Fengjing¹, TENG Weichao^1,2,3,4,5*

1. College of Forestry, Guangxi University, Nanning 530004, China;2. Guangxi Key Laboratory of Forest Ecology and Conservation, Nanning 530004, China;3. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning 530004, China;4. Key Laboratory of National Forestry and Grassland Administration for Fast Growing Tree Breeding and Cultivation in Central and SouthernChina, Nanning 530004, China;5. Guangxi Colleges and Universities Key Laboratory for Forestry Science and Engineering, Nanning 530004, China;6.6. State-Owned Dongmen Forest Farm in Guangxi Zhuang Autonomous Region, Chongzuo 532108, Guangxi, China 1. College of Forestry, Guangxi University, Nanning 530004, China; 2. Guangxi Key Laboratory of Forest Ecology and Conservation, Nanning 530004, China; 3. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning 530004, China; 4. Key Laboratory of National Forestry and Grassland Administration for Fast Growing Tree Breeding and Cultivation in Central and SouthernChina, Nanning 530004, China; 5. Guangxi Colleges and Universities Key Laboratory for Forestry Science and Engineering, Nanning 530004, China; 6. State-Owned Dongmen Forest Farm in Guangxi Zhuang Autonomous Region, Chongzuo 532108, Guangxi, China

Abstract:

To investigate the effect of exogenous nitric oxide(NO)on aluminum tolerance of Eucalyptus seedlings under aluminum stress. In this study, sodium nitroproside(SNP)was used as exogenous NO donor in four kinds of Eucalyptus seedlings(Eucalyptus grandis, E. urophylla, E. tereticornis, E. urophylla&#215;E. grandis). The effects of different concentrations of NO(0, 50, 100, 200, 400, 800 μmol·L^-1)on the physiological indices of ROS, antioxidant enzyme activities and the contents of organic osmotic regulatory substances of Eucalyptus seedlings under 120 mg·L^-1 aluminum stress were studied, and the differences of aluminum tolerance of four Eucalyptus species under NO treatment were compared. The results were as follows:(1)The addition of exogenous NO(50 μmol·L^-1≤NO≤200 μmol·L^-1)under aluminum stress promoted the contents of soluble sugar and soluble protein, the activities of antioxidant enzymes(SOD, POD, CAT, APX), the removal of ROS in the body, the reduction of MDA accumulation, and the improvement of aluminum stress resistance. However, under high concentration of NO(≥800 μmol·L^-1), the activities of antioxidant enzymes and the contents of osmotic regulatory substances decreased in Eucalyptus seedlings, showing stress response.(2)NO significantly improved the aluminum tolerance of sensitive Eucalyptus, but not significantly improved the aluminum tolerance of tolerant Eucalyptus. Finally, the aluminum resistance of four Eucalyptus species tended to be consistent under the action of NO.(3)SOD, MDA, CAT, O₂^-, soluble protein and soluble sugar could be used as key indicators to evaluate the aluminum tolerance of Eucalyptus. This study provides a scientific reference for the selection of aluminum tolerance germplasm resources of Eucalyptus, and laid a foundation for further understanding of the mechanism of NO regulating the difference of aluminum tolerance between Eucalyptus species.

Key words: Eucalyptus, exogenous NO, aluminium stress, physiological indexes, principal component analysis,aluminum tolerance