| 引用本文: | 孟 静, 叶 星, 林鹏程, 杜和艳, 胡星强, 曹雪叶,
王 环, 师生波, 沈建伟, 周党卫.茉莉酸甲酯诱导马尿泡根系分泌物的差异含量及分析[J].广西植物,2025,45(1):172-184.[点击复制] |
| MENG Jing, YE Xing, LIN Pengcheng, DU Heyan, HU Xingqiang, CAO Xueye,
WANG Huan, SHI Shengbo, SHEN Jianwei, ZHOU Dangwei.Differential content and analysis of methyl jasmonate induced root exudates in Przewalskia tangutica[J].Guihaia,2025,45(1):172-184.[点击复制] |
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| 茉莉酸甲酯诱导马尿泡根系分泌物的差异含量及分析 |
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孟 静1, 叶 星1, 林鹏程1, 杜和艳1, 胡星强1, 曹雪叶1,
王 环2, 师生波2, 沈建伟2, 周党卫1,2*
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1. 青海民族大学 药学院, 西宁 810007;2. 中国科学院西北高原生物研究所, 高原生物适应与进化重点实验室, 西宁 810008
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| 摘要: |
| 马尿泡是一种濒危高山植物,产于青藏高原,托烷类生物碱为其主要活性成分。马尿泡的根、种子和全草都可入药,具有重要的药用价值。为探究茉莉酸甲酯诱导下马尿泡根系分泌物的变化,该研究采用LC-MS/MS非靶向代谢组学技术对0、150 μmol·L-1茉莉酸甲酯处理3 d和7 d的马尿泡根系分泌物进行了分析。结果表明:(1)在0、150 μmol·L-1茉莉酸甲酯处理3 d和7 d后,马尿泡根系分泌物含量具有明显差异。(2)与空白处理相比,150 μmol·L-1茉莉酸甲酯处理后,根系分泌物数量明显增加。(3)马尿泡根系分泌物主要参与的KEGG通路有α-亚麻酸代谢通路、植物激素信号转导通路和赖氨酸生物合成通路。综上认为,茉莉酸甲酯诱导影响了马尿泡根系的代谢,改变了马尿泡根系分泌物的含量和数量。该研究通过非靶向代谢组学分析,初步揭示了参与马尿泡根系应答茉莉酸甲酯诱导分泌的关键代谢物,为进一步了解在茉莉酸甲酯诱导下高山植物根系分泌物的变化和代谢机制提供了理论依据,也为马尿泡资源的可持续发展和空间提供了新思路。 |
| 关键词: 马尿泡, 根系分泌物, 茉莉酸甲酯, 非靶向代谢组学, 代谢途径 |
| DOI：10.11931/guihaia.gxzw202407010 |
| 分类号:Q946.8 |
| 文章编号:1000-3142(2025)01-0172-13 |
| 基金项目:青海省创新实验平台项目(2023-ZJ-Y20); 青海省特派员专项(2024-1-NCC-0024) |
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| Differential content and analysis of methyl jasmonate induced root exudates in Przewalskia tangutica |
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MENG Jing1, YE Xing1, LIN Pengcheng1, DU Heyan1, HU Xingqiang1, CAO Xueye1,
WANG Huan2, SHI Shengbo2, SHEN Jianwei2, ZHOU Dangwei1,2*
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1. School of Pharmacy, Qinghai Minzu University, Xining 810007, China;2. Key Laboratory of Adaptation and Evolution of
Plateau Biota(AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
1. School of Pharmacy, Qinghai Minzu University, Xining 810007, China; 2. Key Laboratory of Adaptation and Evolution of
Plateau Biota(AEPB)
, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
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| Abstract: |
| Przewalskia tangutica is an endangered alpine plant, found in the Qinghai-Tibet Plateau, contains tropane alkaloids as its main active ingredients. The roots, seeds and whole herb of P. tangutica are used in medicine and have significant medicinal value. The study was conducted to analyze the changes in the root exudates of P. tangutica induced by methyl jasmonate. The plants were treated with 0, 150 μmol·L-1 methyl jasmonate for 3 d and 7 d, and the root exudates were analyzed using the non-targeted metabolomics technique of LC-MS/MS. The results were as follows:(1)There was a significant difference in the content of root exudates from P. tangutica after 3 d and 7 d treatments with 0, 150 μmol·L-1 methyl jasmonate.(2)The amount of root exudate increased significantly with the 150 μmol·L-1 methyl jasmonate treatment compared to the control.(3)The KEGG pathways mainly involved in the root exudates of P. tangutica were α-linolenic acid metabolism pathway, plant hormone signal transduction pathway and lysine biosynthesis pathway. In summary, it was concluded that the induction of methyl jasmonate affected the metabolism and changed the content and quantity of induced exudates in the root system of P. tangutica. Through non-targeted metabolomics analysis, the study preliminarily reveals the key metabolites involved in the root exudate response to methyl jasmonate of P. tangutica, which provides a theoretical reference for further understanding of the changes in root exudate and metabolism mechanism of this alpine plant under methyl jasmonate. In addition, the results also offer a new way of thinking on the continuation of the resources. |
| Key words: Przewalskia tangutica, root exudate, methyl jasmonate, non-targeted metabolomics, metabolic pathway |
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