引用本文: | 伍 勇, 韦艾骥, 杨 堃, 李金桂, 董思思, 刘大利, 侯春兰,
刘红玲, 程 驰, 薛飞龙, 刘松青.刺梨多糖提取物对小鼠Ⅱ型糖尿病的干预研究[J].广西植物,2023,43(11):2120-2130.[点击复制] |
WU Yong, WEI Aiji, YANG Kun, LI Jingui, DONG Sisi, LIU Dali, HOU Chunlan,
LIU Hongling, CHENG Chi, XUE Feilong, LIU Songqing.Intervention study of roxburgh rose polysaccharide extracts on Type Ⅱ diabetes in mice[J].Guihaia,2023,43(11):2120-2130.[点击复制] |
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刺梨多糖提取物对小鼠Ⅱ型糖尿病的干预研究 |
伍 勇1, 韦艾骥1, 杨 堃1, 李金桂1, 董思思1, 刘大利1, 侯春兰1,
刘红玲1,2, 程 驰1,2, 薛飞龙1,2, 刘松青1,2*
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1. 成都师范学院 化学与生命科学学院, 成都 611130;2. 特色园艺生物资源开发与利用四川省高校重点实验室, 成都 611130
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摘要: |
为探究刺梨多糖(Rosa roxbunghii Tratt. polysaccharide, RRTP)与刺梨不溶性膳食纤维(Rosa roxbunghii Tratt. insoluble dietary fiber, RTIDF)在降血糖功能上是否存在协同作用,该研究对RRTP和RTIDF进行提取、分离和纯化,通过体外实验与体内小鼠Ⅱ型糖尿病的干预实验,测定其抗氧化、降血糖活性,分析体内降血糖功能与小鼠肠道菌群结构关系。结果表明:(1)体外实验发现,RRTP有较好的自由基清除力,能对α-葡萄糖苷酶、α-淀粉酶起显著抑制作用,IC50值分别为0.293、4.251 mg·mL-1,而RTIDF仅表现对α-淀粉酶活性具有一定的抑制作用。(2)刺梨多糖提取物干预模型小鼠后,逆转了肥胖小鼠继续消瘦的趋势,与CK组(生理盐水)相比,RTIDF和RRTP+RTIDF干预的小鼠的血糖水平显著下调,血清中过氧化氢酶活力明显增强,RRTP+RTIDF优于RTIDF。(3)RRTP和RTIDF干预可降低肝脏的炎症因子,缓解细胞肿胀程度,增加盲肠的吸收细胞数目,恢复肠壁黏膜层。(4)分析肠道菌群发现,RTIDF、RRTP可降低拟杆菌门与厚壁菌门的比例,增加醋酸杆菌等有益菌的种群丰度,RTIDF对于种群的调节作用更加显著。综上所述,RRTP与RTIDF在糖尿病小鼠的血糖干预中具有一定的协同作用,或可共同作为改善Ⅱ型糖尿病的干预剂。 |
关键词: 刺梨多糖, 膳食纤维素, 分离纯化, Ⅱ型糖尿病, 血糖干预 |
DOI:10.11931/guihaia.gxzw202206035 |
分类号:Q946 |
文章编号:1000-3142(2023)11-2120-11 |
基金项目:成都师范学院校级科研项目(CS18ZA02); 四川省科技计划项目(2021JDRC0123); 成都师范学院“质量工程”项目(2022JG10,2021-2023); 年四川省高等教育人才培养质量和教学改革项目(JG2021-1376); 成都师范学院专项科研项目(ZZBS2020-13)。 |
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Intervention study of roxburgh rose polysaccharide extracts on Type Ⅱ diabetes in mice |
WU Yong1,2, WEI Aiji1, YANG Kun1, LI Jingui1, DONG Sisi1, LIU Dali1, HOU Chunlan1,
LIU Hongling1,2, CHENG Chi1,2, XUE Feilong1,2, LIU Songqing1,2*
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1. College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China;2. Sichuan Provincial Key Laboratory
for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu 611130, China
1. College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China; 2. Sichuan Provincial Key Laboratory
for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu 611130, China
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Abstract: |
To investigate the synergistic effect of Rosa roxbunghii Tratt polysaccharide(RRTP)and Rosa roxbunghii Tratt insoluble dietary fiber(RTIDF)on hypoglycemic function. In this study, RRTP and RTIDF were extracted, isolated and purified, the antioxidant and hypoglycemic activities of polysaccharides were measured by in vitro experiment and the relationship between hypoglycemic function and intestinal microbiota structure in mice was analyzed in vivo intervention experiment of type Ⅱ diabetes in mice. The results were as follows:(1)RRTP had a good free radical scavenging ability in vitro, and could significantly inhibit α-glucosidase and α-amylase activities with IC50 values of 0.293 and 4.251 mg·mL-1, respectively. RTIDF only showed certain inhibitory activity on α-amylase activity.(2)After the intervention of the extractive in the model mice, the tendency of the obese mice to continue to lose weight was reversed. Compared with CK group, the blood glucose level of RTIDF and RRTP+RTIDF mice were significantly down-regulated, and the activity of CAT enzyme in serum was significantly enhanced. RRTP+RTIDF group was superior to RTIDF group.(3)The extract intervention could reduce the inflammatory factors in the liver, relieve the degree of cell swelling, increase the number of absorbing cells in the cecum, and restore the intestinal wall mucosal layer.(4)Further analysis of intestinal flora showed that RTIDF and RRTP could reduce the proportion of bacteroidetes to firmicutes, increase the abundance of beneficial bacteria such as acetobacter, but RTIDF had more significant regulation effect on the population. Therefore, based on in vitro hypoglycemic simulation and in vivo intervention results, RRTP and RTIDF have a certain synergistic effect on glucose intervention in diabetic mice, it may be used together as an intervention to improve type Ⅱ diabetes. |
Key words: Rosa roxbunghii Tratt polysaccharide, dietary cellulose, isolation and purification, type Ⅱ diabetes, blood glucose intervention |
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