| 摘要: |
| 为探究喀斯特高原峡谷植被恢复对土壤微生物群落结构、土壤有机碳组分变化的影响以及有机碳与微生物的关系，该文以贵州喀斯特高原峡谷耕地 (对照)和典型植被恢复（人工花椒林地和天然次生林）的土壤（0~20 cm）为研究对象，测定土壤有机碳及组分含量、微生物群落组成和多样性，研究了植被恢复后土壤有机碳及组分含量和微生物多样性及群落组成的变化特征，并分析有机碳组分与微生物类群的关系。结果表明：（1）植被恢复显著增加了土壤有机碳及组分含量，且0~10 cm土层相对10~20 cm土层增加更显著（P<0.05）。（2）植被恢复明显改变了土壤微生物β多样性和物种相对丰度，但α多样性并未发生显著变化。（3）所有样品中土壤的主要优势细菌门为未分类细菌门(unclassified.knorank.dBacteria)、放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)和酸杆菌门(Acidobacteriota)，主要优势真菌为子囊菌门(Ascomycota)、未分类真菌门(unclasified.kFungi)、担子菌门(Basidiomycota) 和被孢菌门(Mortiereomycota)；（4）与土壤有机碳及组分密切相关细菌主要为放线菌门中的Asanoa、norank_f_67-14、Solirubrobacter、norank_f_Ilumatobacteraceae、Streptomyces以及子囊菌门中的unclassified_p_Ascomycota、Setophaeosphaeria、unclassified_o_Helotiales、unclassified_o_Pleosporales、Cladosporium、Setophoma、unclassified_o_Sordariales、Metarhizium、Codinaea、Exophiala。该研究表明喀斯特石漠化治理植被恢复可以促进土壤有机碳的积累，改变微生物群落，其中放线菌门和子囊菌门是影响有机碳及组分的变化关键物种。 |
| 关键词: 喀斯特土壤，喀斯特石漠化，植被恢复，土壤有机碳，土壤微生物 |
| DOI：10.11931/guihaia.gxzw202402005 |
| 分类号: |
| Fund project:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| Effects of rocky desertification vegetation restoration on soil microbial community structure and organic carbon in karst plateau canyons |
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LI Jiqiong1, LEI Yanting1, JIANG Xingyu1, LI Rui1, LAN Jiacheng1,2*
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1. School of Geography and Environmental Sciences, Guizhou Normal University, Guiyang 550001, China; 2. Karst Research Institute of Guizhou Normal University/National Karst Rocky Desertification Prevention and Control Engineering Technology Research Center, Guiyang 550001, China
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| Abstract: |
| This study aims to explore the effects of vegetation restoration on the structure of soil microbial communities, the variation in soil organic carbon components, and the interaction between microbes and organic carbon in karst plateau canyons. Soils were collected from 0 to 20 cm depth in cropland (control) and under typical vegetation restoration (Zanthoxyhum planispinum var Dingtanensis and natural secondary forests) in the Guizhou karst plateau canyons. The contents of soil organic carbon and its components and the composition and diversity of microbial communities were determined. Changes in soil organic carbon and its components, microbial diversity, and community composition after vegetation restoration were investigated. The relationships between soil organic carbon components and microbial taxa were analyzed. Results showed that (1) vegetation restoration significantly increased the content of soil organic carbon and its components, particularly in the 0~10 cm soil layer compared with the 10~20 cm layer (P<0.05). (2) Vegetation restoration significantly altered the β-diversity and relative abundance of soil microbial species, although no significant change was observed in α-diversity. (3) The dominant bacterial phyla in all samples were unclassified.knorank.dBacteria, Actinobacteriota, Proteobacteria, and Acidobacteriota, and the main fungal phyla were Ascomycota, unclassified.kFungi, Basidiomycota, and Mortierellomycota. (4) Bacteria closely related to soil organic carbon and its components mainly included Asanoa, norank_f_67-14, Solirubrobacter, norank_f_Ilumatobacteraceae, and Streptomyces from Actinobacteriota and unclassified_p_Ascomycota, Setophaeosphaeria, unclassified_o_Helotiales, unclassified_o_Pleosporales, Cladosporium, Setophoma, unclassified_o_Sordariales, Metarhizium, Codinaea, and Exophiala from Ascomycota.The results showed that vegetation restoration in karst rocky desertification control could promote the accumulation of soil organic carbon and change the microbial community, among which Actinobacteriota and Ascomycota were the key species affecting the change of organic carbon and its components. |
| Key words: Karst soil, karst rocky desertification, vegetation restoration, soil organic carbon, soil microorganism |
