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| 哈茨木霉T9131鉴定及其拮抗病原菌和诱导黄芪抗病的作用分析 |
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牛景萍1, 燕翔1, 白雨果1, 李婉迪1, 石志勇1, 梁建萍1,2*, 李玉芳3, 李飚3, 赵祥4
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1.山西农业大学 生命科学学院,山西 太谷 030801；2.山西农业大学 中兽医药现代化山西省重点实验室,山西 太谷 030801；3.浑源县现代农业发展中心,山西 浑源 037400；4.山西北岳神耆生物科技股份有限公司,山西 浑源 037400
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| 摘要: |
| 木霉是土壤和植物根系中常见的真菌，在农业生物防治中起重要作用。为探究木霉在黄芪抗根腐病中的作用，该研究从黄芪根腐病病根中分离鉴定木霉并研究其对病原菌的拮抗作用和对黄芪抗病生理指标的影响。通过形态学观察、ITS序列和tef1序列分析确定木霉类型；利用平板对峙试验分析分离木霉对腐皮镰刀菌的拮抗作用；测定黄芪生理指标过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和脯氨酸（Pro）来明确分离木霉在诱导黄芪抗病中的作用。结果表明：（1）从黄芪病根中分离鉴定的木霉为哈茨木霉，命名为T9131。（2）哈茨木霉T9131与腐皮镰刀菌HYFS-1平板对峙6 d时T9131对HYFS-1的抑制率为72% ± 1% 。（3）与只接种腐皮镰刀菌HYFS-1相比，当HYFS-1侵染0 h时，T9131显著提高SOD活性；当HYFS-1侵染24 h时，T9131显著提高SOD、POD活性和Pro含量；当HYFS-1侵染48 h时，T9131显著提高POD、PAL活性和Pro含量。综上认为，哈茨木霉T9131可以通过直接抑制病原菌HYFS-1生长和诱导黄芪抗病生理指标SOD、POD、PAL活性和Pro含量来防治黄芪根腐病，为进一步探究哈茨木霉的生防作用和在黄芪抗根腐病中的作用机理奠定基础。 |
| 关键词: 黄芪根腐病，哈茨木霉，腐皮镰刀菌，拮抗作用，生理指标测定 |
| DOI：10.11931/guihaia.gxzw202407012 |
| 分类号: |
| Fund project:山西省现代农业中药材产业技术体系专项（2025年）；2023年度山西省科技成果转化引导专项（202304021301059）；山西省基础研究计划资助项目（20210302124371）；2022年恒山黄芪产业研究院项目（XDHZHQY2022-04）；山西农业大学科技创新基金项目（2018YJ29）。 |
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| Identification of Trichoderma harzianum T9131 and analysis of its effect on against pathogen and disease resistance of Astragalus membranaceus |
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NIU Jingping1, YAN Xiang1, BAI Yuguo1, LI wandi1, SHI Zhiyong1, LIANG Jianping1,2*, LI Yufang3, LI Biao3, Zhao Xiang4
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1. College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China; 2. Shanxi Key Laboratory of Chinese Veterinary Medicine Modernization, Shanxi Agricultural University, Taigu 030801, Shanxi, China; 3. Hunyuan County Modern Agricultural Development Center, Hunyuan 037400, Shanxi, China; 4. Shanxi Beiyue Biological Technology Co., Ltd, Hunyuan 037400, Shanxi, China
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| Abstract: |
| The fungus Trichoderma spp. is ubiquitously detectable in soil and plant roots, and it plays a crucial role in the biological control of agriculture. To explore the influence of Trichoderma spp. on the resistance of Astragalus membranaceus to root rot, the aim of this study is to isolate and identify type of Trichoderma from Astragalus membranaceus root rot, and subsequently investigate its antagonistic confrontation on pathogen and analyze its effect on change of resistance physiological indexes of Astragalus membranaceus. The type of Trichoderma
was determined through morphological characteristics, ITS and tef1 sequence analysis. The antagonistic confrontation of Trichoderma isolate against Fusarium solani HYFS-1 was analyzed by plate confrontation assay. To determine the role of Trichoderma isolate in inducing the resistance of Astragalus membranaceus, the physiological indexes such as catalase (CAT), superoxide dismutase (SOD), phenylalanine ammonlyase (PAL), peroxidase (POD) and proline (Pro) were determined. The results were as follows：(1) The Trichoderma type from Astragalus membranaceus root rot was identified as Trichoderma harzianum and named T9131. (2) The inhibition rate of Trichoderma harzianum T9131 against Fusarium solani HYFS-1 reached 72% ± 1% after 6 days of two confrontation. (3) Compared to HYFS-1 alone, T9131 significantly enhanced SOD activity at 0h after HYFS-1 infection. At the 24h infection, T9131 markedly increased SOD activity, POD activity, and Pro content. By the 48h infection, T9131 significantly elevated POD, PAL activity, and Pro content. To sum up, Trichoderma harzianum T9131 can control Astragalus membranaceus root rot by directly inhibiting the growth of HYFS-1 and could enhance SOD, POD, PAL acitivities and Pro content of Astragalus membranaceus under Fusarium solani HYFS-1 stress. This research will lay the foundation for exploring the mechanism of Trichoderma harzianum against Astragalus membranaceus root rot. was determined through morphological characteristics, ITS and tef1 sequence analysis. The antagonistic confrontation of Trichoderma isolate against Fusarium solani HYFS-1 was analyzed by plate confrontation assay. To determine the role of Trichoderma isolate in inducing the resistance of Astragalus membranaceus, the physiological indexes such as catalase (CAT), superoxide dismutase (SOD), phenylalanine ammonlyase (PAL), peroxidase (POD) and proline (Pro) were determined. The results were presented as follows：(1) The Trichoderma type from Astragalus membranaceus root rot was identified as Trichoderma harzianum and named T9131. (2) The inhibition rate of Trichoderma harzianum T9131 against Fusarium solani HYFS-1 reached 72% ± 1% after 6 days of two confrontation. (3) Compared to HYFS-1 alone, T9131 significantly enhanced SOD activity at 0h after HYFS-1 infection. At the 24h infection, T9131 markedly increased SOD activity, POD activity, and Pro content. By the 48h infection, T9131 significantly elevated POD, PAL activity, and Pro content. To sum up, Trichoderma harzianum T9131 can control Astragalus membranaceus root rot by directly inhibiting the growth of HYFS-1 and could enhance SOD, POD, PAL acitivities and Pro content of Astragalus membranaceus under Fusarium solani HYFS-1 stress. This research will lay the foundation for exploring the mechanism of Trichoderma harzianum against Astragalus membranaceus root rot. |
| Key words: Astragalus membranaceus root rot, Trichoderma harzianum, Fusarium solani, antagonistic confrontation, physiological indexes determination |
