| 摘要: |
| 为研究天名精（Carpesium faberi）植物的化学成分。该研究采用硅胶柱层析、Rp-C18柱层析及制备型高效液相色谱仪（HPLC）等手段对其进行分离和纯化，通过理化性质和波谱数据鉴定化合物结构。结果表明：从天名精全草乙酸乙酯部位浸膏中分离得到11个单体化合物，分别鉴定为4α,10α-二羟基-1β(H),5β(H)-11(13)-烯-8,12-愈创木酚（1）、吐叶醇（2）、(R)-4-((2S,4S)-2,4-二羟基-2,6,6-三甲基环己基)丁基-3-烯-2-酮（3）、carpespene F（4）、4-((1R,2S)-3,3-二甲基-2-(3-氧代丁基)环丁基)正戊基-4-烯-1-乙酸叶醇酯（5）、carpespene I（6）、4β,10β-二羟基-5α(H)-1,11(13)-二烯-8α,12-愈创木酚（7）、木犀草素（8）、(+)-丁香脂素-O-β-D-葡萄糖苷（9）、2-甲氧基-4-(2-丙烯基)苯基β-D-吡喃葡萄糖苷（10）、山奈酚3-O-β-D-葡萄糖苷（11）。其中，化合物5为新化合物，化合物1、4、6、7为愈创木型倍半萜，化合物9、11为首次从该植物中分离得到。该研究成果为天名精的化学成分研究提供理论依据。 |
| 关键词: 天名精，化学成分，分离纯化，结构鉴定，新化合物 |
| DOI：10.11931/guihaia.gxzw202409023 |
| 分类号: |
| Fund project:国家自然科学基金(31760089); 贵州医科大学学术新苗(19NSP083)。 |
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| Separation and identification of chemical constituents of Carpesium faberi |
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Feng Enming1, Chen Jie1, Li Qindan1, ChenLu1, Liu Lulan1, Yan Ying1,2*
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1. Engineering Technology Research Center for Chemical Drug R & D, College of Phammacy, Guizhou Medical University, Guiyang 55004, China; 2. College of Medicine and Health Adminisration, Guichou Medical University, Guiyang 550025, China
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| Abstract: |
| To study the chemical constituents of Carpesium faberi. The compounds were separated and purified by silica gel column chromatography, Rp-C18 column chromatography and preparative high performance liquid chromatography (HPLC), and their structures were identified by physicochemical properties and spectral data. The results were as follows：Eleven monomer compounds were isolated from the extract of C. faberi whole grass ethyl acetate and identified as 4α,10α-dihydroxy-1β(H),5β(H)-guai-11-(13)-en-8,12-olide (1), vomifoliol (2), (R)-4-((2S,4S)-2,4-dihydroxy-2,6,6-trimethylcyclohexylidene)but-3-en-2-one (3), carpespene F(4), 4-((1R,2S)-3,3-dimethyl-2-(3-oxobutyl)cyclobutyl)pent-4-en-1-yl acetate (5), carpespene I (6), 4β,
10α-Dihydroxy-5α(H)-1,11(13)-guaidien-8α,12-olide (7), luteolin (8), (+)-syringaresinol-O-β-D-g
lucopyranoside (9), 2-Methoxy-4-(2-propenyl)-phenyl-β-D-glucopyranoside (10), kaempferol 3-O-β-D-glucopyranoside (11). Compound 5 was a new compound, compounds 1, 4, 6 and 7 were guaiane-type sesquiterpenoids, and compounds 9 and 11 were isolated from this plant for the first time. The research results provide a theoretical basis for the chemical composition of C. faberi. |
| Key words: Carpesium faberi, chemical composition, separation and purification technology, structural identification, new compound |
