| 摘要: |
| 为发掘红树植物内生细菌资源和寻找新型微生物杀虫剂,该研究从北部湾徐闻海域采集7种红树植物共16份样品,设计10种分离培养基,使用稀释涂布法分析红树植物内生细菌的分布特征,通过16S rRNA分子生物学方法对内生细菌进行多样性分析,并利用秀丽隐杆线虫模型,通过杀线虫活性实验测试内生细菌乙酸乙酯提取物的杀线虫活性。结果表明:(1)从16份红树植物各组织器官中获得33株内生细菌,分布于19个科23个属。其中,芽孢杆菌属(Bacillus)为优势菌属,并发现了10株潜在的新种或新属。(2)筛选到具有显著杀线虫活性的菌株IMDGX 4725和IMDGX 4744,半数致死浓度(LC50)分别为61.58、100.89 mg·mL-1。研究结果证实了徐闻海域红树具有多样性丰富的内生细菌,同时部分细菌具有较强的杀线虫活性,具有发现新型微生物杀虫剂的潜力。 |
| 关键词: 内生细菌, 红树林,物种多样性, 杀线虫活性 |
| DOI：10.11931/guihaia.gxzw201905006 |
| 分类号:Q946 |
| 文章编号:1000-3142(2020)03-0301-10 |
| Fund project:国家自然科学基金(21662006, 41566004); 广西自然科学基金面上项目(2018GXNSFAA281268); 广西中医药大学岐黄工程高层次人才团队培育项目(2018006); 广西中医药大学2017年引进博士科研启动基金(2017BS039); 广西中医药大学海洋药物研究院团队科研专项项目(2018ZD005-A06)[Supported by the National Natural Science Foundation of China(21662006, 41566004); Guangxi General Program Natural Foundation(2018GXNSFAA281268); Development Program of High-level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(2018006); Research Launching Fund for Introduced Doctors from Guangxi University of Chinese Medicine in 2017(2017BS039); Special Program for Scientific Research Project under Institutes of Marine Drugs of Guangxi University of Chinese Medicine(2018ZD005-A06)]。 |
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| Diversity and nematicidal activity of endophytic bacteria from mangrove plants collected from Beibu Gulf coast at Xuwen |
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LI Mi, HOU Shishi, YIN Jianglin, HE Yuming, LIU Yonghong, YI Xiangxi*
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Institute of Marine Drugs /Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
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| Abstract: |
| In order to find endophytie bacteria resources and search for new microbial insecticides from mangrove plants, the bacteria were isolated from sixteen samples of seven mangrove plants which collected from Beibu Gulf coast at Xuwen with designed ten kinds of isolation media. The 16S rRNA molecular biology method, dilution coating method and Caenorhabditis elegans screening model were used to explore the diversity and nematicidal activity of endophytic bacteria isolated from mangrove plants. The results were as follows:(1)A total of 33 endophytic bacteria were isolated by 16 organs and tissues which could be classified into 19 families and 23 genera and the dominant genus were Bacillus, and ten potential new species or genera were obtained.(2)Through the nematicidal activity test, two kinds of bacteria with nematicidal activity were obtained. Two endophytic bacteria IMDGX 4725 and IMDGX 4744 had the extremely significant nematicidal activities and the lethal concentrations of 50% were 61.58 and 100.89 mg·mL-1, respectively. The research confirm that mangrove plants collected from Xuwen are rich in endophytic bacteria. At the same time, parts of the bacteria have strong nematicidal activity, which could be a potential source for the discovery of the new microbial insecticides. |
| Key words: endophytic bacteria, mangrove plants, species diversity, nematicidal activity |
